Estimating podocyte number and density using a single histologic section

Journal of the American Society of Nephrology

Volumn 25, Issue 5, 2014, Pages 1118-1129

  Venkatareddy, Madhusudan ^a   Wang, Su ^a   Yang, Yan ^a   Patel, Sanjeevkumar ^a   Wickman, Larysa ^b   Nishizono, Ryuzoh ^a   Chowdhury, Mahboob ^a   Hodgin, Jeffrey ^a   Wiggins, Paul A ^c   Wiggins, Roger C ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; PARAFFIN; PROTEIN ANTIBODY; TRANSCRIPTION FACTOR WILMS TUMOR 1 ANTIBODY; TRANSDUCIN LIKE ENHANCER SPLIT 4 ANTIBODY; UNCLASSIFIED DRUG; NUCLEAR PROTEIN; REPRESSOR PROTEIN; TLE4 PROTEIN, HUMAN; WT1 PROTEIN;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY DETECTION; ARTICLE; CALIBRATION; CALIPER; CELL COUNT; CELL DENSITY; CELL NUCLEUS; CONTROLLED STUDY; FEMALE; GLOMERULOPATHY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMAGING SOFTWARE; IMMUNOFLUORESCENCE MICROSCOPY; KIDNEY BIOPSY; KIDNEY HYPERTROPHY; KIDNEY TRANSPLANTATION; MALE; MOUSE; NONHUMAN; NORMAL VALUE; NUCLEAR SHAPE; NUCLEAR SIZE; PODOCYTE; PRIORITY JOURNAL; RAT; RAT MODEL; SENESCENCE; TISSUE SECTION; ANIMAL; BIOPSY; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOLOGY; LABORATORY DIAGNOSIS; STANDARD; VALIDATION STUDY;

ANIMALS; BIOPSY; CELL COUNT; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HUMANS; MALE; MICE; NUCLEAR PROTEINS; PODOCYTES; RATS; REFERENCE STANDARDS; REPRESSOR PROTEINS; SPECIMEN HANDLING; WT1 PROTEINS;

EID: 84906538163     PISSN: 10466673     EISSN: 15333450     Source Type: Journal    
DOI: 10.1681/ASN.2013080859     Document Type: Article

References (36)

1. Pagtalunan ME, Miller PL, Jumping-Eagle S, Nelson RG, Myers BD, Rennke HG, Coplon NS, Sun L, Meyer TW: Podocyte loss and progressive glomerular injury in type II diabetes. J Clin Invest 99: 342-348, 1997
2. Kriz W, Gretz N, Lemley KV: Progression of glomerular diseases: Is the podocyte the culprit? Kidney Int 54: 687-697, 1998
3. KimYH, GoyalM, Kurnit D,Wharram B,Wiggins J, Holzman L, Kershaw D, Wiggins R: Podocyte depletion and glomerulosclerosis have a direct relationship in the PAN-treated rat. Kidney Int 60: 957-968, 2001
4. Kriz W, LeHir M: Pathways to nephron loss starting from glomerular diseases-insights from animal models. Kidney Int 67: 404-419, 2005
5. Wiggins JE, GoyalM, Sanden SK, WharramBL, Shedden KA,Misek DE, Kuick RD, Wiggins RC: Podocyte hypertrophy, "adaptation," and "decompensation" associated with glomerular enlargement and glomerulosclerosis in the aging rat: Prevention by calorie restriction. J Am Soc Nephrol 16: 2953-2966, 2005
6. Wharram BL, Goyal M, Wiggins JE, Sanden SK, Hussain S, Filipiak WE, Saunders TL, Dysko RC, Kohno K, Holzman LB, Wiggins RC: Podocyte depletion causes glomerulosclerosis: diphtheria toxin-induced podocyte depletion in rats expressing human diphtheria toxin receptor transgene. J AmSoc Nephrol 16: 2941-2952, 2005
7. Wiggins RC: The spectrum of podocytopathies: A unifying view of glomerular diseases. Kidney Int 71: 1205-1214, 2007
8. Fukuda A, Wickman LT, Venkatareddy MP, Sato Y, Chowdhury MA, Wang SQ, Shedden KA, Dysko RC, Wiggins JE, Wiggins RC: Angiotensin II-dependent persistent podocyte loss from destabilized glomeruli causes progression of end stage kidney disease. Kidney Int 81: 40-55, 2012
9. Fukuda A, Chowdhury MA, Venkatareddy MP, Wang SQ, Nishizono R, Suzuki T, Wickman LT,Wiggins JE, Muchayi T, Fingar D, Shedden KA, Inoki K, Wiggins RC: Growth-dependent podocyte failure causes glomerulosclerosis. J Am Soc Nephrol 23: 1351-1363, 2012
10. Meyer TW, Bennett PH, Nelson RG: Podocyte number predicts longterm urinary albumin excretion in Pima Indians with type II diabetes and microalbuminuria. Diabetologia 42: 1341-1344, 1999
11. Steffes MW, Schmidt D, McCrery R, Basgen JM; International Diabetic Nephropathy Study Group: Glomerular cell number in normal subjects and in type 1 diabetic patients. Kidney Int 59: 2104-2113, 2001
12. White KE, Bilous RW, Marshall SM, El Nahas M, Remuzzi G, Piras G, De Cosmo S, Viberti G: Podocyte number in normotensive type 1 diabetic patients with albuminuria. Diabetes 51: 3083-3089, 2002
13. Lemley KV, Lafayette RA, Safai M, Derby G, Blouch K, Squarer A,Myers BD: Podocytopenia and disease severity in IgAnephropathy. Kidney Int 61: 1475-1485, 2002
14. Dalla Vestra M, Masiero A, Roiter AM, Saller A, Crepaldi G, Fioretto P: Is podocyte injury relevant in diabetic nephropathy? Studies in patients with type 2 diabetes. Diabetes 52: 1031-1035, 2003
15. WangG, Lai FM, Kwan BC, Lai KB, ChowKM, Li PK, Szeto CC: Podocyte loss in human hypertensive nephrosclerosis. Am J Hypertens 22: 300-306, 2009
16. White KE, Bilous RW: Estimation of podocyte number: A comparison of methods. Kidney Int 66: 663-667, 2004
17. Basgen JM, Nicholas SB, Mauer M, Rozen S, Nyengaard JR: Comparison of methods for counting cells in the mouse glomerulus. Nephron, Exp Nephrol 103: e139-e148, 2006
18. Nicholas SB, Basgen JM, Sinha S: Using stereologic techniques for podocyte counting in themouse: Shifting the paradigm. Am J Nephrol 33[Suppl 1]: 1-7, 2011
19. White KE: Research into the structure of the kidney glomerulus-making it count. Micron 43: 1001-1009, 2012
20. Lemley KV, Bertram JF, Nicholas SB,White K: Estimation of glomerular podocyte number: A selection of valid methods. J Am Soc Nephrol 24: 1193-1202, 2013
21. Abercrombie M: Estimation of nuclear population from microtome sections. Anat Rec 94: 239-247, 1946
22. Pritchard-Jones K: The Wilms tumour gene, WT1, in normal and abnormal nephrogenesis. Pediatr Nephrol 13: 620-625, 1999
23. Milili M, Gauthier L, Veran J, Mattei MG, Schiff C: A new Groucho TLE4 protein may regulate the repressive activity of Pax5 in human B lymphocytes. Immunology 106: 447-455, 2002
24. Sanden SK,Wiggins JE, GoyalM, Riggs LK,Wiggins RC: Evaluation of a thick and thin section method for estimation of podocyte number, glomerular volume, and glomerular volume per podocyte in rat kidney with Wilms' tumor-1 protein used as a podocyte nuclear marker. J Am Soc Nephrol 14: 2484-2493, 2003
25. Bai XY, Basgen JM: Podocyte number in the maturing rat kidney. Am J Nephrol 33: 91-96, 2011
26. Hooke DH, Hancock WW, Gee DC, Kraft N, Atkins RC: Monoclonal antibody analysis of glomerular hypercellularity in human glomerulonephritis. Clin Nephrol 22: 163-168, 1984
27. Hiki Y, Leong AS, Mathew TH, Seymour AE, Pascoe V, Woodroffe AJ: Typing of intraglomerular mononuclear cells associated with transplant glomerular rejection. Clin Nephrol 26: 244-249, 1986
28. Markovic-Lipkovski J, Müller CA, Risler T, Bohle A, Müller GA: Association of glomerular and interstitial mononuclear leukocytes with different forms of glomerulonephritis.Nephrol Dial Transplant 5: 10-17, 1990
29. Lu TC, He JC, Klotman PE: Podocytes in HIV-associated nephropathy. Nephron Clin Pract 106: c67-c71, 2007
30. Gebeshuber CA, Kornauth C, Dong L, Sierig R, Seibler J, Reiss M, Tauber S, Bilban M, Wang S, Kain R, Böhmig GA, Moeller MJ, Gröne HJ, Englert C, Martinez J, Kerjaschki D: Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation ofWT1. Nat Med 19: 481-487, 2013
31. Gregory T: Genome size evolution in animals. In: The Evolution of the Genome, Vol. 1, edited by Gregory T, London, Elsevier Academic Press, 2005, pp 4-87
32. Lasagni L, Lazzeri E, Shankland SJ, Anders HJ, Romagnani P: Podocyte mitosis-a catastrophe. Curr Mol Med 13: 13-23, 2013
33. Webster M, Witkin KL, Cohen-Fix O: Sizing up the nucleus: Nuclear shape, size and nuclear-envelope assembly. JCell Sci 122: 1477-1486, 2009
34. Weibel ER, Gomez DM: A principle for counting tissue structures on random sections. J Appl Physiol 17: 343-348, 1962
35. Weibel ER: Elementary introduction to stereological principles. In: Stereologic Methods: Practical Methods for Biologic Morphometry, London, Academic Press Inc, 1979, pp 40-116, 415
36. Hilliard JE: The calculation of the mean caliper diameter of a body for use in the analysis of the number of particles per unit volume. In: Stereology, edited by Elias H, New York, Springer-Verlag, 1967, pp 211-215