Improvement of organophosphorus hydrolase activity toward nerve agents by amino acid substitutions

Forensic Toxicology

Volumn 32, Issue 2, 2014, Pages 208-213

  Nakayama, Kosuke ^a   Ishikawa, Satoshi ^a   Kawahara, Kazuyoshi ^a   Ohmori, Takeshi ^b   Seto, Yasuo ^b  

^a KANTO GAKUIN UNIVERSITY   (Japan)

^b NATIONAL RESEARCH INSTITUTE OF POLICE SCIENCE   (Japan)

Author keywords

Amino acid substitution; Decontamination; Nerve agents; Organophosphorus hydrolase; Sarin; VX

Indexed keywords

ARYLDIALKYLPHOSPHATASE; CENTRAL NERVOUS SYSTEM AGENTS; COBALT; HISTIDINE; TYROSINE; ZINC ION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME DEGRADATION; HYDROLYSIS; OPH GENE; POINT MUTATION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; WILD TYPE;

EID: 84906330539     PISSN: 18608965     EISSN: 18608973     Source Type: Journal    
DOI: 10.1007/s11419-013-0223-4     Document Type: Article

References (22)

1. Sekiguchi H, Matsushita K, Yamashiro S, Sano Y, Seto Y, Okuda T, Sato A (2006) On-site determination of nerve and mustard gases using a field-portable gas chromatograph-mass spectrometer. Forensic Toxicol 24:17-22 (Pubitemid 44257285)
2. Sano Y, Yamashiro S, Komano A, Maruko H, Sekiguchi H, Takayama Y, Sekioka R, Tsuge K, Ohsawa I, Kanamori-Kataoka M, Seto Y, Satoh A (2007) Detection of proteinous toxins using the Bio-Threat Alart system, part 3: effects of heat pretreatment and intrerfering substances. Forensic Toxicol 25:76-79
3. Yamashiro S, Sano Y, Komano A, Maruko H, Sekiguchi H, Takayama Y, Sekioka R, Tsuge K, Ohsawa I, Kanamori-Kataoka M, Seto Y, Satoh A (2007) Detection of proteinous toxins using the Bio-Threat Alert system, part 4. Differences in detectability according to manufactural lots and according to toxin subtypes. Forensic Toxicol 25:80-83
4. 63Ni radioactive ionization for chemical warfare agents. Forensic Toxicol 28:84-95
5. Komano A, Maruko H, Sekiguchi H, Seto Y (2011) Detection of saxitoxin in counterterrorism using a commercial lateral flow immunoassay kit. Forensic Toxicol 29:38-43
6. Seto Y (2011) Research and development of on-site decontamination system for biological and chemical warfare agents. J Health Sci 57:311-333
7. Ohmori T, Kawahara K, Nakayama K, Shioda A, Ishikawa S, Kanamori-Kataoka M, Kishi S, Komano A, Seto Y (2013) Decontamination of nerve agents by immobilized organophosphorus hydrolase. Forensic Toxicol 31:37-43
8. Dumas DP, Caldwell SR, Wild JR, Raushel FM (1989) Purification and properties of the phosphotriesterase from Pseudomonas diminuta. J Biol Chem 264:19659-19665 (Pubitemid 20008072)
9. Mulbry WW, Karns JS, Kearney PC, Nelson JO, McDaniel CS, Wild JR (1986) Identification of a plasmid-borne parathion hydrolase gene from Flavobacterium sp. by southern hybridization with opd from Pseudomonas diminuta. Appl Environ Microbiol 51:926-930 (Pubitemid 16078585)
10. Harper LL, McDaniel CS, Miller CE, Wild JR (1988) Dissimilar plasmids isolated from Pseudomonas diminuta MG and a Flavobacterium sp. (ATCC 27551) contain identical opd genes. Appl Environ Microbiol 54:2586-2589
11. McDaniel CS, Harper LL, Wild JR (1988) Cloning and sequencing of a plasmid-borne gene (opd) encoding a phosphotriesterase. J Bacteriol 170:2306-2311
12. Omburo GA, Kuo JM, Mullins LS, Raushel FM (1992) Characterization of the zinc binding site of bacterial phosphotriesterase. J Biol Chem 267:13278-13283
13. Dumas DP, Durst HD, Landis WG, Raushel FM, Wild JR (1990) Inactivation of organophosphorus nerve agents by the phosphotriesterase from Pseudomonas diminuta. Arch Biochem Biophys 277:155-159 (Pubitemid 20078899)
14. Lai K, Stolowich NJ, Wild JR (1995) Characterization of P-S bond hydrolysis in organophosphorothioate pesticides by organophosphorus hydrolase. Arch Biochem Biophys 318:59-64
15. Ashani Y, Leader H, Rothschild N, Dosoretz C (1998) Combined effect of organophosphorus hydrolase and oxime on the reactivation rate of diethylphosphoryl-acetylcholinesterase conjugates. Biochem Pharmacol 55:159-168 (Pubitemid 28006182)
16. Dumas DP, Wild JR, Raushel FM (1989) Diisopropylfluorophosphate hydrolysis by an organophosphate anhydrase from Pseudomonas diminuta. Biotech Appl Biochem 11:235-243 (Pubitemid 19095735)
17. Hoskin FCG, Walker JE, Dettbarn WD, Wild JR (1995) Hydrolysis of tetriso by an enzyme derived from Pseudomonas diminuta as a model for the detoxication of O-ethyl S-(2-diisopropylaminoethyl) methylphosphonothiolate (VX). Biochem Pharmacol 49:711-715
18. Kawahara K, Tanaka A, Yoon J, Yokota A (2010) Reclassification of a parathione-degrading Flavobacterium sp. ATCC 27551 as Sphingobium fuliginis. J Gen Appl Microbiol 56:249-255
19. Funhoff EG, Ljusberg J, Wang Y, Andersson G, Averill BA (2001) Mutational analysis of the interaction between active site residues and the loop region in mammalian purple acid phosphatases. Biochemistry 40:11614-11622 (Pubitemid 32911306)
20. Gopal S, Rastogi V, Ashman W, Mulbry W (2000) Mutagenesis of organophosphorus hydrolase to enhance hydrolysis of the nerve agent VX. Biochem Biophys Res Commun 279:516-519 (Pubitemid 32016747)
21. Hill CM, Li WS, Thoden JB, Holden HM, Raushel FM (2003) Enhanced degradation of chemical warfare agents through molecular engineering of the phosphotriesterase active site. J Am Chem Soc 125:8990-8991 (Pubitemid 36903801)
22. Reeves TE, Wales ME, Grimsley JK, Li P, Cerasoli DM, Wild JR (2008) Balancing the stability and the catalytic specificities of OP hydrolases with enhanced V-agent activities. Protein Eng Des Sel 21:405-412 (Pubitemid 351786659)