False positives in multiplex PCR-based next-generation sequencing have unique signatures

Journal of Molecular Diagnostics

Volumn 16, Issue 5, 2014, Pages 541-549

  McCall, Chad M ^a   Mosier, Stacy ^a   Thiess, Michele ^a   Debeljak, Marija ^a   Pallavajjala, Aparna ^a   Beierl, Katie ^a   Deak, Kristen L ^b   Datto, Michael B ^b   Gocke, Christopher D ^a   Lin, Ming Tseh ^a   Eshleman, James R ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

B RAF KINASE; EPIDERMAL GROWTH FACTOR RECEPTOR; K RAS PROTEIN;

AMPLICON; ARTICLE; BINDING SITE; COLORECTAL CARCINOMA; CONTROLLED STUDY; EXON; FALSE POSITIVE RESULT; G873R GENE; GENE; GENE AMPLIFICATION; GENE MUTATION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC IDENTIFICATION; HUMAN; HUMAN TISSUE; LUNG ADENOCARCINOMA; MULTIPLEX POLYMERASE CHAIN REACTION; NEXT GENERATION SEQUENCING; SEQUENCE ANALYSIS; ALGORITHM; BIOLOGY; GENETICS; HIGH THROUGHPUT SEQUENCING; MUTATION; PROCEDURES; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; STANDARDS;

ALGORITHMS; COMPUTATIONAL BIOLOGY; EXONS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MULTIPLEX POLYMERASE CHAIN REACTION; MUTATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY;

EID: 84906236965     PISSN: 15251578     EISSN: 19437811     Source Type: Journal    
DOI: 10.1016/j.jmoldx.2014.06.001     Document Type: Article

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