A fluorescent carbapenem for structure function studies of penicillin-binding proteins, β-lactamases, and β-lactam sensors

Analytical Biochemistry

Volumn 463, Issue , 2014, Pages 70-74

  June, Cynthia M ^a   Vaughan, Robert M ^a   Ulberg, Lucas S ^a   Bonomo, Robert A ^b   Witucki, Laurie A ^a   Leonard, David A ^a  

^a GRAND VALLEY STATE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Carbapenem; Fluorescent modification; Penicillin binding protein; Lactamase

Indexed keywords

ACYLATION; AMIDES; ELECTROPHORESIS; PROBES; PROTEINS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

CARBAPENEMS; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT MODIFICATION; LACTAMASES; PENICILLIN- BINDING PROTEINS; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; STRUCTURE-FUNCTION STUDIES; VISIBLE LIGHT EXCITATION;

FLUORESCENCE;

BETA LACTAM; BETA LACTAMASE; CARBAPENEM; CARBAPENEMASE; FLUORESCEIN; FLUORESCEIN ISOTHIOCYANATE; MEROPENEM; PENICILLIN BINDING PROTEIN; FLUORESCENT DYE; BACTERIAL PROTEIN; BOCILLIN FL; BORON DERIVATIVE; CARBAPENEM DERIVATIVE; PENICILLIN DERIVATIVE; THIENAMYCIN DERIVATIVE;

ARTICLE; DRUG STRUCTURE; MUTANT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; ACYLATION; ACYLATION AND DEACYLATION; AMINO ACID SUBSTITUTION; ANISOTROPY; BINDING AFFINITY; CATALYSIS; DRUG PROTEIN BINDING; HYDROLYSIS; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; SENSOR; STRUCTURE ACTIVITY RELATION; BIOCATALYSIS; CHEMISTRY; KINETICS; METABOLISM;

BACTERIAL PROTEINS; BETA-LACTAMASES; BIOCATALYSIS; BORON COMPOUNDS; CARBAPENEMS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCEIN-5-ISOTHIOCYANATE; HYDROLYSIS; KINETICS; PENICILLIN-BINDING PROTEINS; PENICILLINS; THIENAMYCINS;

EID: 84905837157     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.07.012     Document Type: Article

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