Comparison of pre-analytical FFPE sample preparation methods and their impact on massively parallel sequencing in routine diagnostics

PLoS ONE

Volumn 9, Issue 8, 2014, Pages

  Heydt, Carina ^a   Fassunke, Jana ^a   Künstlinger, Helen ^a   Ihle, Michaela Angelika ^a   König, Katharina ^a   Heukamp, Lukas Carl ^a   Schildhaus, Hans Ulrich ^b   Odenthal, Margarete ^a   Büttner, Reinhard ^a   Merkelbach Bruse, Sabine ^a  

^a UNIVERSITY HOSPITAL OF COLOGNE   (Germany)

^b UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; EPIDERMAL GROWTH FACTOR RECEPTOR; FLUORESCENT DYE; FORMALDEHYDE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HFE PROTEIN; PARAFFIN;

ARTICLE; AUTOMATED DNA EXTRACTION SYSTEM; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA DETERMINATION; DNA EXTRACTION; DNA SEQUENCE; EGFR GENE; EMBEDDING; FORMALIN FIXATION PARAFFIN EMBEDDING; GAPDH GENE; GENERAL MEDICAL DEVICE; HEALTH CARE COST; HFE GENE; HUMAN; HUMAN CELL; HUMAN TISSUE; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; MUTATIONAL ANALYSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; RELIABILITY; SPECTROPHOTOMETER; ULTRAVIOLET SPECTROPHOTOMETRY; VALIDATION PROCESS; CHEMISTRY; COMPARATIVE STUDY; GENETICS; HIGH THROUGHPUT SEQUENCING; ISOLATION AND PURIFICATION; NUCLEOTIDE SEQUENCE; PROCEDURES; TISSUE FIXATION;

DNA; DNA MUTATIONAL ANALYSIS; FORMALDEHYDE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; PARAFFIN EMBEDDING; SEQUENCE ANALYSIS, DNA; TISSUE FIXATION;

EID: 84905668712     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0104566     Document Type: Article

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