A multiplex high-throughput gene expression assay to simultaneously detect disease and functional markers in induced pluripotent stem cell-derived retinal pigment epithelium

Stem Cells Translational Medicine

Volumn 3, Issue 8, 2014, Pages 911-922

  Ferrer, Marc ^a   Corneo, Barbara ^b   Davis, Janine ^c   Wan, Qin ^c   Miyagishima, Kiyoharu Joshua ^c   King, Rebecca ^a   Maminishkis, Arvydas ^c   Marugan, Juan ^a   Sharma, Ruchi ^c   Shure, Michael ^d   Temple, Sally ^b   Miller, Sheldon ^c   Bharti, Kapil ^c  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b Neural Stem Cell Institute   (United States)

^c NATIONAL EYE INSTITUTE   (United States)

^d Thermo Fisher Scientific   (United States)

Author keywords

Gene expression; Induced pluripotency; iPS; Reprogramming; Retina; Retinal pigmented epithelium; Stem cell

Indexed keywords

ACTIVIN A; EZRIN; KRUPPEL LIKE FACTOR 4; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; OLIGONUCLEOTIDE; ORGANIC CATION TRANSPORTER 3; RAB27A PROTEIN; RETINOID RELATED ORPHAN RECEPTOR BETA; SINGLE STRANDED DNA; STAGE SPECIFIC EMBRYO ANTIGEN 4; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR PAX6; TRANSCRIPTION FACTOR SOX2; TRANSCRIPTION FACTOR SOX9; TRANSIENT RECEPTOR POTENTIAL CHANNEL M1; TRANSIENT RECEPTOR POTENTIAL CHANNEL M3;

ADULT; ARTICLE; CALCIUM CELL LEVEL; CELL LYSATE; CONTROLLED STUDY; DISEASE MARKER; DNA PROBE; ECTODERM; ELECTRON MICROSCOPY; ELECTROPHYSIOLOGY; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION ASSAY; HIGH THROUGHPUT SCREENING; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MESODERM; ONCOGENE C MYC; PIGMENT EPITHELIUM; PLURIPOTENT STEM CELL; QUANTIGENE PLEX ASSAY; RETINA DEGENERATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; YOUNG ADULT;

GENE EXPRESSION; INDUCED PLURIPOTENCY; IPS; REPROGRAMMING; RETINA; RETINAL PIGMENTED EPITHELIUM; STEM CELL;

CELL DIFFERENTIATION; CELL LINEAGE; CELL PROLIFERATION; CELLS, CULTURED; DRUG DISCOVERY; EPITHELIAL CELLS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC MARKERS; GENETIC PREDISPOSITION TO DISEASE; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LIMIT OF DETECTION; MULTIPLEX POLYMERASE CHAIN REACTION; PHENOTYPE; REPRODUCIBILITY OF RESULTS; RETINAL DEGENERATION; RETINAL PIGMENT EPITHELIUM; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84905484384     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2013-0192     Document Type: Article

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