Inhibition of protease activity by antisense RNA improves recombinant protein production in Nicotiana tabacum cv. Bright Yellow 2 (BY-2) suspension cells

Biotechnology Journal

Volumn 9, Issue 8, 2014, Pages 1065-1073

  Mandal, Manoj K ^a   Fischer, Rainer ^a,b   Schillberg, Stefan ^b   Schiermeyer, Andreas ^b  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

Author keywords

Antisense RNA; BY 2 cells; Gene silencing; Plant molecular farming; Recombinant protein degradation

Indexed keywords

AMINO ACIDS; ANTIBODIES; CELLS; CHAINS; CLONING; CYTOLOGY; ENZYME ACTIVITY; PLANT CELL CULTURE; PROTEOLYSIS; RNA; TOBACCO;

ANTISENSE RNA; BY-2 CELL; GENE SILENCING; MOLECULAR FARMING; PLANT CELL SUSPENSION CULTURES; PROTEOLYTIC ACTIVITIES; RECOMBINANT PROTEIN PRODUCTIONS; SEQUENCE INFORMATIONS;

RECOMBINANT PROTEINS;

COMPLEMENTARY RNA; GLYCOPROTEIN GP 41; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G1 ANTIBODY 2F5 HEAVY CHAIN; PROTEINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; 2F5 MONOCLONAL ANTIBODY; IMMUNOGLOBULIN KAPPA CHAIN; MONOCLONAL ANTIBODY; PEPTIDE HYDROLASE; VEGETABLE PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CULTIVAR; ENZYME ACTIVITY; ENZYME INHIBITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SUSPENSION CELL CULTURE; TOBACCO; CHEMICAL STRUCTURE; CULTURE TECHNIQUE; ENZYMOLOGY; GENETICS; METABOLISM; PROTEIN SECONDARY STRUCTURE; TRANSGENIC PLANT;

HUMAN IMMUNODEFICIENCY VIRUS 1; NICOTIANA TABACUM;

ANTIBODIES, MONOCLONAL; CELL CULTURE TECHNIQUES; HIV ENVELOPE PROTEIN GP41; IMMUNOGLOBULIN KAPPA-CHAINS; MODELS, MOLECULAR; PEPTIDE HYDROLASES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; RNA, ANTISENSE; TOBACCO;

EID: 84905366886     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300424     Document Type: Article

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