Comparison of Simplexa flu A/B & RSV PCR with cytospin- immunofluorescence and laboratory-developed TaqMan PCR in predominantly adult hospitalized patients

Journal of Clinical Microbiology

Volumn 52, Issue 8, 2014, Pages 3057-3059

  Landry, Marie L ^a,b   Ferguson, David ^b  

^a YALE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b YALE NEW HAVEN HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CONTROLLED STUDY; CYTOSPIN DIRECT FLUORESCENT ANTIBODY ASSAY; DIAGNOSTIC TEST ACCURACY STUDY; FLUORESCENT ANTIBODY TECHNIQUE; HOSPITAL PATIENT; HUMAN; IMMUNOFLUORESCENCE TEST; INFLUENZA A; INFLUENZA B; INFLUENZA VIRUS A; INFLUENZA VIRUS B; INTERMETHOD COMPARISON; LABORATORY TEST; NONHUMAN; NOSE SMEAR; POLYMERASE CHAIN REACTION SYSTEM; PRIORITY JOURNAL; PROCESS DEVELOPMENT; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY SYNCYTIAL VIRUS INFECTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; AGED; COMPARATIVE STUDY; DIAGNOSTIC TEST; EVALUATION STUDY; HOSPITAL; INFLUENZA, HUMAN; MIDDLE AGED; NASOPHARYNX; POLYMERASE CHAIN REACTION; PROCEDURES; VERY ELDERLY; VIROLOGY;

ADULT; AGED; AGED, 80 AND OVER; DIAGNOSTIC TESTS, ROUTINE; FLUORESCENT ANTIBODY TECHNIQUE; HOSPITALS; HUMANS; INFLUENZA, HUMAN; MIDDLE AGED; NASOPHARYNX; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 84905242660     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00738-14     Document Type: Article

References (9)

1. Landry ML. 2011. Diagnostic tests for influenza infection. Curr. Opin. Pediatr. 23:91-97. http://dx.doi.org/10.1097/MOP.0b013e328341ebd9.
2. Landry ML, Ferguson D. 2010. Cytospin-enhanced immunofluorescence and impact of sample quality on detection of novel swine origin (H1N1) influenza virus. J. Clin. Microbiol. 48:957-959. http://dx.doi.org/10.1128/JCM.01678-09.
3. Landry ML, Ferguson D. 2000. SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining. J. Clin. Microbiol. 38:708-711. (Pubitemid 30082667)
4. van Elden LJ, van Loon AM, van der Beek A, Hendriksen KA, Hoepelman AI, van Kraaij MG, Schipper P, Nijhuis M. 2003. Applicability of a real-time quantitative PCR assay for diagnosis of respiratory syncytial virus infection in immunocompromised adults. J. Clin. Microbiol. 41:4378-4381. http://dx.doi.org/10.1128/JCM.41.9.4378-4381.2003. (Pubitemid 37099710)
5. Alby K, Popowitch EB, Miller MB. 2013. Comparative evaluation of the Nanosphere Verigene RV+ assay and the Simplexa Flu A/B & RSV kit for detection of influenza and respiratory syncytial viruses. J. Clin. Microbiol. 51:352-353. http://dx.doi.org/10.1128/JCM.02504-12.
6. Ko SY, Jang JW, Song DJ, Lim CS, Kim WJ. 2013. Evaluation of the Simplexa Flu A/B and RSV test for the rapid detection of influenza viruses. J. Med. Virol. 85:2160-2164. http://dx.doi.org/10.1002/jmv.23712.
7. Selvaraju SB, Tierney D, Leber AL, Patel A, Earley AK, Jaiswal D, Menegus MA. 2014. Influenza and respiratory syncytial virus detection in clinical specimens without nucleic acid extraction using FOCUS direct disc assay is substantially equivalent to the traditional methods and the FOCUS nucleic acid extraction-dependent RT-PCR assay. Diagn. Microbiol. Infect. Dis. 78:232-236. http://dx.doi.org/10.1016/j.diagmicrobio.2013.11.016.
8. Hindiyeh M, Kolet L, Meningher T, Weil M, Mendelson E, Mandelboim M. 2013. Evaluation of Simplexa Flu A/B & RSV for direct detection of influenza viruses (A and B) and respiratory syncytial virus in patient clinical samples. J. Clin. Microbiol. 51:2421-2424. http://dx.doi.org/10.1128/JCM.00286-13.
9. Woodberry MW, Shankar R, Cent A, Jerome KR, Kuypers J. 2013. Comparison of the Simplexa Flu A/B & RSV direct assay and laboratory-developed real-time PCR assays for detection of respiratory virus. J. Clin. Microbiol. 51:3883-3885. http://dx.doi.org/10.1128/JCM.02395-13.