Evaluation of Simplexa flu A/B & RSV for direct detection of influenza viruses (A and B) and respiratory syncytial virus in patient clinical samples

Journal of Clinical Microbiology

Volumn 51, Issue 7, 2013, Pages 2421-2424

  Hindiyeh, Musa ^a   Kolet, Liat ^a   Meningher, Tal ^a,b   Weil, Merav ^a   Mendelson, Ella ^a,c   Mandelboim, Michal ^a  

^a MINISTRY OF HEALTH   (Israel)

^b BAR ILAN UNIVERSITY   (Israel)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS RNA;

ARTICLE; ASSAY; CONTROLLED STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; HUMAN; HUMAN TISSUE; INFLUENZA VIRUS A; INFLUENZA VIRUS A H1N1; INFLUENZA VIRUS B; LABORATORY DEVELOPED ASSAY; MAJOR CLINICAL STUDY; PREDICTIVE VALUE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; CLASSIFICATION; GENETICS; HUMAN RESPIRATORY SYNCYTIAL VIRUS; INFLUENZA, HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MOLECULAR DIAGNOSIS; ORTHOMYXOVIRUS; PROCEDURES; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RETROSPECTIVE STUDY; VIROLOGY;

ORTHOMYXOVIRIDAE; RESPIRATORY SYNCYTIAL VIRUS; RICE STRIPE VIRUS;

HUMANS; INFLUENZA, HUMAN; MOLECULAR DIAGNOSTIC TECHNIQUES; ORTHOMYXOVIRIDAE; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; RETROSPECTIVE STUDIES; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; VIROLOGY;

EID: 84879441334     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00286-13     Document Type: Article

References (13)

1. Mahony JB, Petrich A, Smieja M. 2011. Molecular diagnosis of respiratory virus infections. Crit. Rev. Clin. Lab. Sci. 48:217-249.
2. Mahony JB. 2010. Nucleic acid amplification-based diagnosis of respiratory virus infections. Expert Rev. Anti Infect. Ther. 8:1273-1292.
3. Popow-Kraupp T, Aberle JH. 2011. Diagnosis of respiratory syncytial virus infection. Open Microbiol. J. 5:128-134.
4. Hindiyeh M, Hillyard DR, Carroll KC. 2001. Evaluation of the Prodesse Hexaplex multiplex PCR assay for direct detection of seven respiratory viruses in clinical specimens. Am. J. Clin. Pathol. 116:218-224.
5. Loeffelholz MJ, Pong DL, Pyles RB, Xiong Y, Miller AL, Bufton KK, Chonmaitree T. 2011. Comparison of the FilmArray Respiratory Panel and Prodesse real-time PCR assays for detection of respiratory pathogens. J. Clin. Microbiol. 49:4083-4088.
6. Chen Y, Cui D, Zheng S, Yang S, Tong J, Yang D, Fan J, Zhang J, Lou B, Li X, Zhuge X, Ye B, Chen B, Mao W, Tan Y, Xu G, Chen Z, Chen N, Li L. 2011. Simultaneous detection of influenza A, influenza B, and respiratory syncytial viruses and subtyping of influenza A H3N2 virus and H1N1 (2009) virus by multiplex real-time PCR. J. Clin. Microbiol. 49: 1653-1656.
7. Ginocchio CC, Zhang F, Manji R, Arora S, Bornfreund M, Falk L, Lotlikar M, Kowerska M, Becker G, Korologos D, de Geronimo M, Crawford JM. 2009. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. J. Clin. Virol. 45:191-195.
8. Panning M, Eickmann M, Landt O, Monazahian M, Olschlager S, Baumgarte S, Reischl U, Wenzel JJ, Niller HH, Gunther S, Hollmann B, Huzly D, Drexler JF, Helmer A, Becker S, Matz B, Eis-Hubinger A, Drosten C. 2009. Detection of influenza A(H1N1)v virus by real-time RT-PCR. Euro Surveill. 4(36):pii-19329. http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId-19329.
9. Hu A, Colella M, Tam JS, Rappaport R, Cheng SM. 2003. Simultaneous detection, subgrouping, and quantitation of respiratory syncytial virus A and B by real-time PCR. J. Clin. Microbiol. 41:149-154.
10. WHO. 2009. CDC protocol of realtime RTPCR for influenza A(H1N1). WHO Collaborating Centre for Influenza, CDC, Atlanta, GA. http://www.who.int/csr/ resources/publications/swineflu/CDCRealtimeRTPCR-S wineH1Assay-2009-20090430. pdf.
11. Hindiyeh M, Levy V, Azar R, Varsano N, Regev L, Shalev Y, Grossman Z, Mendelson E. 2005. Evaluation of a multiplex real-time reverse transcriptase PCR assay for detection and differentiation of influenza viruses A and B during the 2001-2002 influenza season in Israel. J. Clin. Microbiol. 43:589-595.
12. Pierce VM, Hodinka RL. 2012. Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children. J. Clin. Microbiol. 50:3458-3465.
13. Alby K, Popowitch EB, Miller MB. 2013. Comparative evaluation of the Nanosphere Verigene RV + assay and the Simplexa Flu A/B & RSV kit for detection of influenza and respiratory syncytial viruses. J. Clin. Microbiol. 51:352-353.