Influenza and respiratory syncytial virus detection in clinical specimens without nucleic acid extraction using FOCUS direct disc assay is substantially equivalent to the traditional methods and the FOCUS nucleic acid extraction-dependent RT-PCR assay

Diagnostic Microbiology and Infectious Disease

Volumn 78, Issue 3, 2014, Pages 232-236

  Selvaraju, Suresh B ^a   Tierney, David ^d   Leber, Amy L ^b   Patel, Anami ^c   Earley, Amalia K ^a   Jaiswal, Dipeshkumar ^d   Menegus, Marilyn A ^a  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b NATIONWIDE CHILDREN S HOSPITAL   (United States)

^c LE BONHEUR CHILDREN S HOSPITAL   (United States)

^d Focus Diagnostics Inc   (United States)

Author keywords

Direct Disc assay; Flu and RSV detection; FOCUS nucleic acid extraction dependent assay; Nasopharyngeal swab specimens; Simplexa

Indexed keywords

DIRECT DISC ASSAY; FLU AND RSV DETECTION; FOCUS NUCLEIC ACID EXTRACTION-DEPENDENT ASSAY; NASOPHARYNGEAL SWAB SPECIMENS; SIMPLEXA;

ADOLESCENT; ADULT; AUSTRALIA; CHILD; CHILD, PRESCHOOL; DNA, VIRAL; FEMALE; HUMANS; INFLUENZA B VIRUS; INFLUENZA, HUMAN; MALE; MIDDLE AGED; PROSPECTIVE STUDIES; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUSES; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; UNITED STATES; YOUNG ADULT;

EID: 84893735021     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2013.11.016     Document Type: Article

References (19)

1. Beck E.T., Jurgens L.A., Kehl S.C., Bose M.E., Patitucci T., LaGue E., et al. Development of a rapid automated influenza A, influenza B, and respiratory syncytial virus A/B multiplex real-time RT-PCR assay and its use during the 2009 H1N1 swine-origin influenza virus epidemic in Milwaukee, Wisconsin. J Mol Diagn 2010, 12:74-81.
2. Centers for Disease Control and Prevention (CDC) Evaluation of rapid influenza diagnostic tests for detection of novel influenza A (H1N1) virus: United States, 2009. Morb Mortal Wkly Rep 2009, 58:826-829.
3. Centers for Disease Control and Prevention (CDC) Respiratory syncytial virus-United States, July 2007-June 2011. Morb Mortal Wkly Rep 2011, 60:1203-1206.
4. Centers for Disease Control and Prevention (CDC). Seasonal Influenza - Questions & Answers. Accessed July 4th, 2012. http://www.cdc.gov/flu/about/qa/disease.htm.
5. Cruz A.T., Cazacu A.C., McBride L.J., Greer J.M., Demmler G.J. Performance characteristics of a rapid immunochromatographic assay for detection of influenza virus in children during the 2003 to 2004 influenza season. Ann Emerg Med 2006, 47:250-254.
6. Falsey A.R., Formica M.A., Treanor J.J., Walsh E.E. Comparison of quantitative reverse transcription-PCR to viral culture for assessment of respiratory syncytial virus shedding. J Clin Microbiol 2003, 41:4160-4165.
7. Falsey A.R., Formica M.A., Walsh E.E. Diagnosis of respiratory syncytial virus infection: comparison of reverse transcription-PCR to viral culture and serology in adults with respiratory illness. J Clin Microbiol 2002, 40:817-820.
8. Falsey A.R., Walsh E.E. Respiratory syncytial virus infection in elderly adults. Drugs Aging 2005, 22:577-587.
9. Ginocchio C.C., Zhang F., Manji R., Arora S., Bornfreund M., Falk L., et al. Evaluation of multiple test methods for the detection of the novel 2009 influenza A (H1N1) during the New York City outbreak. J Clin Virol 2009, 45:191-195.
10. Gueudin M., Vabret A., Petitjean J., Gouarin S., Brouard J., Freymuth F. Quantitation of respiratory syncytial virus RNA in nasal aspirates of children by real-time RT-PCR assay. J Virol Methods 2003, 109:39-45.
11. Hymes W.C., Mills A., Ferguson S., Langer J., She R.C., Mahoney W., et al. Development of a multiplex real-time RT-PCR assay for detection of influenza A, influenza B, RSV and typing of the 2009-H1N1 influenza virus. J Virol Methods 2010, 167:113-118.
12. Iwane M.K., Edwards K.M., Szilagyi P.G., Walker F.J., Griffin M.R., Weinberg G.A., et al. Population-based surveillance for hospitalizations associated with respiratory syncytial virus, influenza virus, and parainfluenza viruses among young children. Pediatrics 2004, 113:1758-1764.
13. Kuypers J., Wright N., Morrow R. Evaluation of quantitative and type-specific real-time RT-PCR assays for detection of respiratory syncytial virus in respiratory specimens from children. J Clin Virol 2004, 31:123-129.
14. Newton D.W., Mellen C.F., Baxter B.D., Atmar R.L., Menegus M.A. Practical and sensitive screening strategy for detection of influenza virus. J Clin Microbiol 2002, 40:4353-4356.
15. Novak-Weekley S.M., Marlowe E.M., Poulter M., Dwyer D., Speers D., Rawlinson W., et al. Evaluation of the Cepheid Xpert Flu assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1 and influenza B viruses. J Clin Microbiol 2012, 50:1704-1710.
16. Ohm-Smith M.J., Nassos P.S., Haller B.L. Evaluation of the Binax NOW, BD Directigen, and BD Directigen EZ assays for detection of respiratory syncytial virus. J Clin Microbiol 2004, 42:2996-2999.
17. Selvaraju S.B., Bambach A.V., Leber A.L., Patru M.M., Patel A., Menegus M.A. Comparison of the Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependant assay) and the Prodessa ProFlu+™ assay for detecting influenza and respiratory syncytial viruses. Diag Microbiol Infect Dis 2013, http://dx.doi.org/ 10.1016/j.diagmicrobio.2013.11.015.
18. Selvaraju S.B., Selvarangan R. Evaluation of three influenza A and B real-time reverse transcription-PCR assays and a new 2009 H1N1 assay for detection of influenza viruses. J Clin Microbiol 2010, 48:3870-3875.
19. Walsh E.E., Falsey A.R. Respiratory syncytial virus infection in adult populations. Infect Disord Drug Targets 2012, 12:98-102.