Serine phosphorylation on position 1033 of vinculin impacts cellular mechanics

Biochemical and Biophysical Research Communications

Volumn 450, Issue 2, 2014, Pages 1095-1098

  Auernheimer, Vera ^a   Goldmann, Wolfgang H ^a  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

Focal adhesions; FRAP; Magnetic tweezer; pS1033; Traction microscopy; Vinculin

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; SERINE; VINCULIN;

2D TRACTION MICROSCOPY; ANIMAL CELL; ARTICLE; CELL MUTANT; CONTROLLED STUDY; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; FOCAL ADHESION; MICROSCOPY; MOLECULAR MECHANICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; REACTION TIME; RECOVERY TIME; RIGIDITY; WILD TYPE;

FOCAL ADHESIONS; FRAP; MAGNETIC TWEEZER; PS1033; TRACTION MICROSCOPY; VINCULIN;

ANIMALS; FIBROBLASTS; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; FOCAL ADHESIONS; MAGNETIC PHENOMENA; MICE, KNOCKOUT; MUTATION; PHOSPHORYLATION; SERINE; STRESS, MECHANICAL; VINCULIN;

EID: 84905110929     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.06.122     Document Type: Article

References (23)

1. N.Q. Balaban, U.S. Schwarz, D. Riveline, P. Goichberg, G. Tzur, I. Sabanay, D. Mahalu, S. Safran, A. Bershadsky, L. Addadi, and B. Geiger Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates Nat. Cell Biol. 3 2001 466 472 (Pubitemid 32422082)
2. D.K. Werth, J.E. Niedel, and I. Pastan Vinculin, a cytoskeletal substrate of protein kinase-C J. Biol. Chem. 258 1983 1423 1426
3. X. Peng, E.S. Nelson, J.L. Maiers, and K.A. DeMali New insights into vinculin function and regulation Int. Rev. Cell Mol. Biol. 287 2011 191 231
4. J. Golji, T. Wendorff, and M.R. Mofrad Phosphorylation primes vinculin for activation Biophys. J. 102 2012 2022 2030
5. J. Golji, and M.R. Mofrad A molecular dynamics investigation of vinculin activation Biophys. J. 99 2010 1073 1081
6. J. Golji, J. Lam, and M.R. Mofrad Vinculin activation is necessary for complete talin binding Biophys. J. 100 2011 332 340
7. C.S. Izzard A precursor of the focal contact in cultured fibroblasts Cell Motil. Cytoskeleton 10 1988 137 142
8. C. Möhl, N. Kirchgessner, C. Schäfer, K. Küpper, S. Born, G. Diez, W.H. Goldmann, R. Merkel, and B. Hoffmann Becoming stable and strong: the interplay between vinculin exchange dynamics and adhesion strength during adhesion site maturation Cell Motil. Cytoskeleton 66 2009 350 364
9. Z. Zhang, G. Izaguirre, S.Y. Lin, H.Y. Lee, E. Schaefer, and B. Haimovich The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading Mol. Biol. Cell 15 2004 4234 4247 (Pubitemid 39122026)
10. S. Moese, M. Selbach, V. Brinkmann, A. Karlas, B. Haimovich, S. Backert, and T.F. Meyer The Helicobacter pylori CagA protein disrupts matrix adhesion of gastric epithelial cells by dephosphorylation of vinculin Cell. Microbiol. 9 2007 1148 1161 (Pubitemid 46547135)
11. W.H. Ziegler, U. Tigges, A. Zieseniss, and B.M. Jockusch A lipid-regulated docking site on vinculin for protein kinase C J. Biol. Chem. 277 2002 7396 7404 (Pubitemid 34953136)
12. K.A. DeMali Vinculin-a dynamic regulator of cell adhesion Trends Biochem. Sci. 29 2004 565 567
13. C.T. Mierke, P. Kollmannsberger, D.P. Zitterbart, G. Diez, T.M. Koch, S. Marg, W.H. Ziegler, W.H. Goldmann, and B. Fabry Vinculin facilitates cell invasion into three-dimensional collagen matrices J. Biol. Chem. 285 2010 13121 13130
14. G. Diez, V. Auernheimer, B. Fabry, and W.H. Goldmann Head/tail interaction of vinculin influences cell mechanical behavior Biochem. Biophys. Res. Commun. 406 2011 85 88
15. P. Kollmannsberger, and B. Fabry High-force magnetic tweezers with force feedback for biological applications Rev. Sci. Instrum. 78 2007 114301 114306
16. F.J. Alenghat, B. Fabry, K.Y. Tsai, W.H. Goldmann, and D.E. Ingber Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer Biochem. Biophys. Res. Commun. 277 2000 93 99
17. C.T. Mierke, P. Kollmannsberger, D. Paranhos-Zitterbart, J. Smith, B. Fabry, and W.H. Goldmann Mechano-coupling and regulation of contractility by the vinculin tail domain Biophys. J. 94 2008 661 670
18. R.J. Pelham Jr., and Y. Wang Cell locomotion and focal adhesions are regulated by substrate flexibility Proc. Natl. Acad. Sci. U.S.A. 94 1997 13661 13665 (Pubitemid 28009639)
19. J.P. Butler, I.M. Tolic-Norrelykke, B. Fabry, and J.J. Fredberg Traction fields, moments, and strain energy that cells exert on their surroundings Am. J. Physiol. Cell Physiol. 282 2002 C595 C605
20. R. Janostiak, J. Brabek, V. Auernheimer, Z. Tatarova, L.A. Lautscham, T. Dey, J. Gemperle, R. Merkel, W.H. Goldmann, B. Fabry, and D. Rosel CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics Cell. Mol. Life Sci. 71 2014 727 744
21. K. Küpper, N. Lang, C. Möhl, N. Kirchgessner, S. Born, W.H. Goldmann, R. Merkel, and B. Hoffmann Tyrosine phosphorylation of vinculin at position 1065 modifies focal adhesion dynamics and cell tractions Biochem. Biophys. Res. Commun. 399 2010 560 564
22. G. Diez, P. Kollmannsberger, C.T. Mierke, T.M. Koch, H. Vali, B. Fabry, and W.H. Goldmann Anchorage of vinculin to lipid membranes influences cell mechanical properties Biophys. J. 97 2009 3105 3112
23. V.F. Wirth, F. List, G. Diez, and W.H. Goldmann Vinculin's C-terminal region facilitates phospholipid membrane insertion Biochem. Biophys. Res. Commun. 398 2010 433 437