Droplet interface bilayer reconstitution and activity measurement of the mechanosensitive channel of large conductance from Escherichia coli

Journal of the Royal Society Interface

Volumn 11, Issue 98, 2014, Pages

  Barriga, Hanna M G ^a   Booth, Paula ^b   Haylock, Stuart ^a   Bazin, Richard ^c   Templer, Richard H ^a   Ces, Oscar ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

^c PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United Kingdom)

Author keywords

1,2 Dioleoyl sn glycero 3 phosphocholine; Droplet interface bilayers; Fluorescence; Mechanosensitive channel of large conductance

Indexed keywords

AMINO ACIDS; BIOLOGICAL MEMBRANES; ESCHERICHIA COLI; FLUORESCENCE; LIPIDS; METHANE;

1 ,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; ACTIVITY MEASUREMENTS; BI-LAYER; DROPLET INTERFACE BILAYERS; MECHANOSENSITIVE CHANNEL; MEMBRANE PROTEINS; REACTIVE CHEMICALS; SYNTHETIC LIPID;

DROPS;

BROMINE DERIVATIVE; CALCEIN; DIOLEOYLPHOSPHATIDYLCHOLINE; GLYCEROPHOSPHORYLCHOLINE DERIVATIVE; MEMBRANE PROTEIN; (2-(TRIMETHYLAMMONIUM)ETHYL)METHANETHIOSULFONATE; 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE; 1,2-DIPHYTANOYLPHOSPHATIDYLCHOLINE; ESCHERICHIA COLI PROTEIN; FLUORESCENT DYE; GLYCEROPHOSPHORYLCHOLINE; ION CHANNEL; LIPID; LIPID BILAYER; MESYLIC ACID DERIVATIVE; MSCL PROTEIN, E COLI; PHOSPHATIDYLCHOLINE;

ARTICLE; BILAYER MEMBRANE; CONDUCTANCE; CONTROLLED STUDY; DROPLET INTERFACE BILAYER; ESCHERICHIA COLI; FLUORESCENCE; MECHANOSENSITIVE CHANNEL OF LARGE CONDUCTANCE; NONHUMAN; ANALOGS AND DERIVATIVES; CHEMISTRY; LIPID BILAYER;

ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; GLYCERYLPHOSPHORYLCHOLINE; ION CHANNELS; LIPID BILAYERS; LIPIDS; MESYLATES; PHOSPHATIDYLCHOLINES;

EID: 84905028661     PISSN: 17425689     EISSN: 17425662     Source Type: Journal    
DOI: 10.1098/rsif.2014.0404     Document Type: Article

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