Cloning and characterization of Pangasianodon hypophthalmus growth hormone gene and its heterologous expression

Applied Biochemistry and Biotechnology

Volumn 173, Issue 6, 2014, Pages 1446-1468

  Sekar, Megarajan ^a,b   Singh, Shiva Dhar ^a,c   Gupta, Subodh ^a  

^a CENTRAL INSTITUTE OF FISHERIES EDUCATION   (India)

^b CENTRAL MARINE FISHERIES RESEARCH INSTITUTE   (India)

^c INDIAN COUNCIL OF AGRICULTURAL RESEARCH   (India)

Author keywords

Aquaculture; Growth enhancement; Recombinant growth hormone; Tissue specific expression; Transgenic fish

Indexed keywords

AMINO ACIDS; AQUACULTURE; ESCHERICHIA COLI; FISH; GENE ENCODING; HISTOLOGY; PLANTS (BOTANY); TISSUE;

GROWTH ENHANCEMENT; GROWTH HORMONE GENES; GROWTH HORMONES; HETEROLOGOUS EXPRESSION; SEQUENCE SIMILARITY; TISSUE-SPECIFIC EXPRESSION; TRANSGENIC FISHES; UNTRANSLATED REGIONS;

GENE EXPRESSION;

AMINO ACID; AP 1 PROTEIN; CCAAT BINDING FACTOR; COMPLEMENTARY DNA; CRE RECOMBINASE; CREBP PROTEIN; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; GROWTH HORMONE; PIT 1A PROTEIN; PROTEIN; RECOMBINANT GROWTH HORMONE; SIGNAL PEPTIDE; TATA BINDING PROTEIN; TBP PROTEIN; TRANSCRIPTION FACTOR PIT 1; TRANSCRIPTION FACTOR SP1; UNCLASSIFIED DRUG; FISH PROTEIN; HYBRID PROTEIN; MESSENGER RNA;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; ATLANTIC COD; BINDING SITE; BRAIN; CATFISH; CONSENSUS SEQUENCE; CYPRINIFORMES; DISULFIDE BOND; DNA SEQUENCE; EEL; ESCHERICHIA COLI; EXON; EXPRESSION VECTOR; GENE SEQUENCE; GENETIC DIFFERENCE; GILL; GLYCOSYLATION; HETEROLOGOUS EXPRESSION; INTRON; LIVER; MOLECULAR CLONING; MOLECULAR PHYLOGENY; MOLECULAR WEIGHT; MUSCLE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OVARY; PANGASIANODON HYPOPHTHALMUS; PANGASIUS PANGASIUS; PERCIFORMES; PHYLOGENETIC TREE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN SECONDARY STRUCTURE; REAL TIME POLYMERASE CHAIN REACTION; SALMONIFORMES; SEQUENCE ANALYSIS; SILURIFORMES; START CODON; STOP CODON; TATA BOX; TRANSCRIPTION INITIATION SITE; WESTERN BLOTTING; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; GENE EXPRESSION; GENETICS; METABOLISM; MOLECULAR GENETICS; PHYLOGENY; PROTEIN TERTIARY STRUCTURE; TISSUE DISTRIBUTION;

CYPRINIFORMES; ESCHERICHIA COLI; PANGASIANODON HYPOPHTHALMUS; PISCES; SILURIFORMES;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATFISHES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; FISH PROTEINS; GENE EXPRESSION; GROWTH HORMONE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; RNA, MESSENGER; TISSUE DISTRIBUTION;

EID: 84904767906     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-014-0946-7     Document Type: Article

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