Improvement of stability of nitrile hydratase via protein fragment swapping

Biochemical and Biophysical Research Communications

Volumn 450, Issue 1, 2014, Pages 401-408

  Cui, Youtian ^a   Cui, Wenjing ^a   Liu, Zhongmei ^a   Zhou, Li ^a   Kobayashi, Michihiko ^b   Zhou, Zhemin ^a  

^a JIANGNAN UNIVERSITY   (China)

^b UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Enzyme stability; Fragment swapping; Homologous recombination; Molecular dynamics; Nitrile hydratase

Indexed keywords

BACTERIAL PROTEIN; CHIMERIC PROTEIN; NITRILE HYDRATASE;

ARTICLE; CHIMERA; CIRCULAR DICHROISM; COMAMONAS TESTOSTERONI; CONTROLLED STUDY; ENZYME STABILITY; HYDRATION; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PSEUDOMONAS PUTIDA; PSEUDONOCARDIA THERMOPHILA; PSEUDONOCARDIACEAE; THERMOSTABILITY; WILD TYPE;

COMAMONAS TESTOSTERONI; PSEUDOMONAS PUTIDA; PSEUDONOCARDIA THERMOPHILA;

ENZYME STABILITY; FRAGMENT SWAPPING; HOMOLOGOUS RECOMBINATION; MOLECULAR DYNAMICS; NITRILE HYDRATASE;

AMINO ACID SEQUENCE; BINDING SITES; ENZYME ACTIVATION; ENZYME STABILITY; HYDRO-LYASES; MODELS, CHEMICAL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; PROTEIN ENGINEERING; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 84904720709     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.05.127     Document Type: Article

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