Comparative effects of adding β-mercaptoethanol or L-ascorbic acid to culture or vitrification-warming media on IVF porcine embryos

Reproduction, Fertility and Development

Volumn 26, Issue 6, 2014, Pages 875-882

  Castillo Martín, Miriam ^a   Bonet, Sergi ^a   Morató, Roser ^a   Yeste, Marc ^b  

^a UNIVERSITY OF GIRONA   (Spain)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

antioxidants; apoptosis; blastocysts; cryotolerance; reactive oxygen species

Indexed keywords

ASCORBIC ACID; MERCAPTOETHANOL; REACTIVE OXYGEN METABOLITE; ANTIOXIDANT; CULTURE MEDIUM;

ADD ON THERAPY; ANIMAL TISSUE; APOPTOSIS; ARTICLE; BLASTOCYST; CELL COUNT; CONTROLLED STUDY; EMBRYO; EMBRYO CULTURE; EMBRYO DEVELOPMENT; FERTILIZATION IN VITRO; IN VITRO STUDY; NONHUMAN; PORCINE EMBRYO; SURVIVAL RATE; VITRIFICATION; WARMING; ANIMAL; COMPARATIVE STUDY; CRYOPRESERVATION; CULTURE MEDIUM; DRUG EFFECTS; FEMALE; MALE; METABOLISM; PHARMACOLOGY; PIG; PROCEDURES; VETERINARY;

ANIMALS; ANTIOXIDANTS; ASCORBIC ACID; BLASTOCYST; CRYOPRESERVATION; CULTURE MEDIA; EMBRYO CULTURE TECHNIQUES; EMBRYONIC DEVELOPMENT; FEMALE; FERTILIZATION IN VITRO; MALE; MERCAPTOETHANOL; REACTIVE OXYGEN SPECIES; SWINE; VITRIFICATION;

EID: 84904707436     PISSN: 10313613     EISSN: 14485990     Source Type: Journal    
DOI: 10.1071/RD13116     Document Type: Article

References (43)

1. Booth, P. J., Holm, P., and Callesen, H. (2005). The effect of oxygen tension on porcine embryonic development is dependent on embryo type. Theriogenology 63, 2040-2052. doi:10.1016/J.THERIOGENOLOGY. 2004.10.001
2. Brison, D. R., and Schultz, R. M. (1997). Apoptosis during mouse blastocyst formation: evidence for a role for survival factors including transforming growth factor alpha. Biol. Reprod. 56, 1088-1096. doi:10.1095/ BIOLREPROD56.5.1088
3. Buettner, G. R. (1993). The pecking order of free radicals and antioxidants: lipid peroxidation, alpha-tocopherol, and ascorbate. Arch. Biochem. Biophys. 300, 535-543. doi:10.1006/ABBI.1993.1074
4. Byrne, A. T., Southgate, J., Brison, D. R., and Leese, H. J. (1999). Analysis of apoptosis in the preimplantation bovine embryo using TUNEL. J. Reprod. Fertil. 117, 97-105. doi:10.1530/JRF.0.1170097
5. Castillo-Martín, M., Yeste, M., Morató, R., Mogas, T., and Bonet, S. (2013). Cryotolerance of in vitro-produced porcine blastocysts is improved when using glucose instead of pyruvate and lactate during the first 2 days of embryo culture. Reprod. Fertil. Dev. 25, 737-745. doi:10.1071/ RD12117
6. Chung, Y. G., Mann, M. R., Bartolomei, M. S., and Latham, K. E. (2002). Nuclear-cytoplasmic 'tug of war' during cloning: effects of somatic cell nuclei on culture medium preferences of preimplantation cloned mouse embryos. Biol. Reprod. 66, 1178-1184. doi:10.1095/BIOLREPROD66. 4.1178
7. Goto, Y., Noda, Y., Mori, T., and Nakano, M. (1993). Increased generation of reactive oxygen species in embryos cultured in vitro. Free Radic. Biol. Med. 15, 69-75. doi:10.1016/0891-5849(93)90126-F
8. Guérin, P., El Mouatassim, S., and Ménézo, Y. (2001). Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum. Reprod. Update 7, 175-189. doi:10.1093/HUMUPD/7.2.175
9. Gupta, M. K., Uhm, S. J., and Lee, H. T. (2010). Effect of vitrification and beta-mercaptoethanol on reactive oxygen species activity and in vitro development of oocytes vitrified before or after in vitro fertilization. Fertil. Steril. 93, 2602-2607. doi:10.1016/J.FERTNSTERT.2010.01.043
10. Halliwell, B., and Gutteridge, J. M. C. (1989). The chemistry of oxygen radicals and other derived species. In 'Free Radicals in Biology and Medicine'. (Eds B. Halliwell and J. M. C. Gutteridge.) pp. 22-85. (Clarendon Press: Oxford, UK.)
11. Hammami, S., Morató, R., Romaguera, R., Roura, M., Catalá, M.G., Paramio, M.T., Mogas, T., and Izquierdo, D. (2012). Developmental competence and embryo quality of small oocytes from pre-pubertal goats cultured in IVM medium supplemented with low level of hormones, insulin-transferring- selenium and ascorbic acid. Reprod. Dom. Anim. 48, 339-344. doi:10.1111/J.1439- 0531.2012.02160.X
12. Hashem, A., Hossein, M. S., Woo, J. Y., Kim, S., Kim, J. H., Lee, S. H., Koo, O. J., Park, S. M., Lee, E. G., Kang, S. K., and Lee, B. C. (2006). Effect of potassium simplex optimization medium and NCSU 23 supplemented with beta-mercaptoethanol and amino acids of in vitro fertilized porcine embryos. J. Reprod. Dev. 52, 591-599. doi:10.1262/JRD.18010
13. Hossein, M. S., Hashem, M. A., Jeong, Y. W., Lee, M. S., Kim, S., Kim, J. H., Koo, O. J., Park, S. M., Lee, E. G., Park, S. W., Kang, S. K., Lee, B. C., and Hwang, W. S. (2007). Temporal effects of alpha-tocopherol and L-ascorbic acid on in vitro fertilized porcine embryo development. Anim. Reprod. Sci. 100, 107-117. doi:10.1016/J.ANIREPROSCI.2006.06.013
14. Hosseini, S.M., Forouzanfar, M., Hajian, M., Asgari, V., Abedi, P., Hosseini, L., Ostadhosseini, S., Moulavi, F., Safahani Langrroodi, M., Sadeghi, H., Bahramian, H., Eghbalsaied, Sh., and Nasr-Esfahani,M. H. (2009). Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important? J. Assist. Reprod. Genet. 26, 355-364. doi:10.1007/ S10815-009-9317-7
15. Hu, J., Cheng, D., Gao, X., Bao, J., Ma, X., and Wang, H. (2012). Vitamin C enhances the in vitro development of porcine pre-implantation embryos by reducing oxidative stress. Reprod. Domest. Anim. 47, 873-879. doi:10.1111/J.1439-0531.2011.01982.X
16. Huang, Y., Tang, X., Xie, W., Zhou, Y., Li, D., Zhou, Y., Zhu, J., Yuan, T., Lai, L., Pang, D., and Ouyang, H. (2011). Vitamin C enhances in vitro and in vivo development of porcine somatic cell nuclear transfer embryos. Biochem. Biophys. Res. Commun. 411, 397-401. doi:10.1016/J.BBRC.2011.06.160
17. Jeong, Y. W., Park, S. W., Hossein, M. S., Kim, S., Kim, J. H., Lee, S. H., Kang, S. K., Lee, B. C., and Hwang, W. S. (2006). Antiapoptotic and embryotrophic effects of alpha-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer. Theriogenology 66, 2104-2112. doi:10.1016/ J.THERIOGENOLOGY.2006.06.007
18. Karja, N. W., Kikuchi, K., Fahrudin, M., Ozawa, M., Somfai, T., Ohnuma, K., Noguchi, J., Kaneko, H., and Nagai, T. (2006). Development to the blastocyst stage, the oxidative state, and the quality of early developmental stage of porcine embryos cultured in alteration of glucose concentrations in vitro under different oxygen tensions. Reprod. Biol. Endocrinol. 4, 54. doi:10.1186/1477- 7827-4-54
19. Kere, M., Siriboon, C., Lo, N. W., Nguyen, N. T., and Ju, J. C. (2013). Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation. J. Reprod. Dev. 59, 78-84
20. Kikuchi, K., Onishi, A., Kashiwazaki, N., Iwamoto, M., Noguchi, J., Kaneko, H., Akita, T., and Nagai, T. (2002). Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biol. Reprod. 66, 1033-1041. doi:10.1095/BIOLREPROD66.4.1033
21. Kitagawa, Y., Suzuki, K., Yoneda, A., and Watanabe, T. (2004). Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology 62, 1186-1197. doi:10.1016/J.THERIOGENOLOGY.2004.01.011
22. Korhonen, K., Julkunen, H., Kananen, K., Bredbacka, P., Tiirikka, T., Räty, M., Vartia, K., Kaimio, I., Kontinen, A., Halmekytö, M., Vilkki, J., Peippo, J., and Lindeberg, H. (2012). The effect of ascorbic acid during biopsy and cryopreservation on viability of bovine embryos produced in vivo. Theriogenology 77, 201-205. doi:10.1016/J.THERIO GENOLOGY.2011.07.034
23. Kuwayama, M., Vajta, G., Kato, O., and Leibo, S. P. (2005). Highly efficient vitrification method for cryopreservation of human oocytes. Reprod. Biomed. Online 11, 300-308. doi:10.1016/S1472-6483(10)60837-1
24. Lane, M., Maybach, J. M., and Gardner, D. K. (2002). Addition of ascorbate during cryopreservation stimulates subsequent embryo development. Hum. Reprod. 17, 2686-2693. doi:10.1093/HUMREP/17.10.2686
25. Mazur, P. (1970). Cryobiology: the freezing of biological systems. Science 168, 939-949. doi:10.1126/SCIENCE.168.3934.939
26. Nakayama, T., Noda, Y., Goto, Y., and Mori, T. (1994). Effects of visible light and other environmental factors on the production of oxygen radicals by hamster embryos. Theriogenology 41, 499-510. doi:10.1016/0093-691X(94)90086-X
27. Nedambale, T. L., Du, F., Yang, X., and Tian, X. C. (2006). Higher survival rate of vitrified and thawed in vitro produced bovine blastocysts following culture in defined medium supplemented with b-mercaptoethanol. Anim. Reprod. Sci. 93, 61-75. doi:10.1016/J.ANIREPROSCI. 2005.06.027
28. Noda, Y., Matsumoto, H., Umaoka, Y., Tatsumi, K., Kishi, J., and Mori, T. (1991). Involvement of superoxide radicals in the mouse two-cell block. Mol. Reprod. Dev. 28, 356-360. doi:10.1002/MRD.1080280408
29. Park, J. I., Grant, C. M., Davies, M. J., and Dawes, I. W. (1998). The cytoplasmic Cu, Zn superoxide dismutase of saccharomyces cerevisiae is required for resistance to freeze-thaw stress. Generation of free radicals during freezing and thawing. J. Biol. Chem. 273, 22 921-22 928. doi:10.1074/JBC.273.36. 22921
30. Petters, R. M., and Wells, K. D. (1993). Culture of pig embryos. J. Reprod. Fertil. Suppl. 48, 61-73
31. Rath, D., Long, C. R., Dobrinsky, J. R., Welch, G. R., Schreier, L. L., and Johnson, L. A. (1999). In vitro production of sexed embryos for gender preselection: high-speed sorting of X-chromosome-bearing sperm to produce pigs after embryo transfer. J. Anim. Sci. 77, 3346-3352
32. Robertson, I., and Nelson, R. E. (1998). Certification and identification of the embryo. In 'Manual of the International Embryo Transfer Society'. (Eds D. A. Stringfellow and S. M. Seidel.) pp. 103-134. (International Embryo Transfer Society: Savoy, IL.)
33. Romek, M., Gajda, B., Krzysztofowicz, E., Kepczynski, M., and Somorag, Z. (2011). New technique to quantify the lipid composition of lipid droplets in porcine oocytes and pre-implantation embryos using Nile Red fluorescent probe. Theriogenology 75, 42-54. doi:10.1016/J.THER IOGENOLOGY.2010.06.040
34. Rose, R. C., and Bode, A. M. (1993). Biology of free radical scavengers: an evaluation of ascorbate. FASEB J. 7, 1135-1142
35. Shamsuddin, M., Larson, B., Gustafsson, H., and Rodrigues-Martinez, H. (1994). A serum-free, cell-free culture system for development of bovine one-cell embryo up to blastocyst stage with improved viability. Theriogenology 41, 1033-1043. doi:10.1016/S0093-691X(05)80026-3
36. Somfai, T., Ozawa, M., Noguchi, J., Kaneko, H., Kuriani Karja, N. W., Farhudin, M., Dinnyés, A., Nagai, T., and Kikuchi, K. (2007). Developmental competence of in vitro-fertilized porcine oocytes after in vitro maturation and solid surface vitrification: effect of cryopreservation on oocyte antioxidative system and cell cycle stage. Cryobiology 55, 115-126. doi:10.1016/J.CRYOBIOL.2007.06.008
37. Stachowiak, E. M., Papis, K., Kruszewski, M., Iwanenko, T., Bartlomiejczyk, T., and Modlinski, J. A. (2009). Comparison of the level(s) of DNA damage using Comet assay in bovine oocytes subjected to selected vitrification methods. Reprod. Domest. Anim. 44, 653-658. doi:10.1111/ J.1439-0531.2007.01042.X
38. Takahashi, M., Nagai, T., Hamano, S., Kuwayama, M., Okamura, N., and Okano, A. (1993). Effect of thiol compounds on in vitro development and intracellular glutathione content of bovine embryos. Biol. Reprod. 49, 228-232. doi:10.1095/BIOLREPROD49.2.228
39. Tarín, J. J., and Trounson, A. O. (1993). Effects of stimulation or inhibition of lipid peroxidation on freezing-thawing of mouse embryos. Biol. Reprod. 49, 1362-1368. doi:10.1095/BIOLREPROD49.6.1362
40. Vajta, G. (2000). Vitrification of the oocytes and embryos of domestic animals. Anim. Reprod. Sci. 60-61, 357-364. doi:10.1016/S0378-4320 (00)00097-X
41. Wang, X., Falcone, T., Attaran, M., Goldberg, J. M., Agarwal, A., and Sharma, R. K. (2002). Vitamin C and vitamin E supplementation reduce oxidative stress-induced embryo toxicity and improve the blastocyst development rate. Fertil. Steril. 78, 1272-1277. doi:10.1016/ S0015-0282(02)04236-X
42. Yamanaka, K., Sugimura, S., Wakai, T., Kawahara, M., and Sato, E. (2009). Difference in sensitivity to culture conditions between in vitro fertilized and somatic cell nuclear transfer embryos in pigs. J. Reprod. Dev. 55, 299-304. doi:10.1262/JRD.20174
43. Yang, H. W., Hwang, K. J., Kwon, H. C., Kim, H. S., Choi, K. W., and Oh, K. S. (1998). Detection of reactive oxygen species (ROS) and apoptosis in human fragmented embryos. Hum. Reprod. 13, 998-1002. doi:10.1093/ HUMREP/13.4.998