메뉴 건너뛰기




Volumn 75, Issue 1, 2011, Pages 42-54

New technique to quantify the lipid composition of lipid droplets in porcine oocytes and pre-implantation embryos using Nile Red fluorescent probe

Author keywords

Confocal microscopy; Lipids; Nile Red; Oocyte; Pig; Pre implantation embryo

Indexed keywords

CHOLESTEROL; NILE RED; OXAZINE DERIVATIVE; PHOSPHOLIPID; TRIACYLGLYCEROL;

EID: 78649876881     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2010.06.040     Document Type: Article
Times cited : (48)

References (46)
  • 2
    • 33645295821 scopus 로고    scopus 로고
    • Relationship between bovine oocyte morphology and in vitro developmental potential
    • Nagano M., Katagiri S., Takahashi Y. Relationship between bovine oocyte morphology and in vitro developmental potential. Zygote 2006, 14:53-61.
    • (2006) Zygote , vol.14 , pp. 53-61
    • Nagano, M.1    Katagiri, S.2    Takahashi, Y.3
  • 3
    • 67651172790 scopus 로고    scopus 로고
    • Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos
    • Jeong W.J., Chao S.J., Lee H.S., Deb G.K., Lee Y.S., Kwon T.H., Kong I.K. Effect of cytoplasmic lipid content on in vitro developmental efficiency of bovine IVP embryos. Theriogenology 2009, 72:584-589.
    • (2009) Theriogenology , vol.72 , pp. 584-589
    • Jeong, W.J.1    Chao, S.J.2    Lee, H.S.3    Deb, G.K.4    Lee, Y.S.5    Kwon, T.H.6    Kong, I.K.7
  • 5
    • 0031324792 scopus 로고    scopus 로고
    • Cryopreservation of pig embryos
    • Dobrinsky J.R. Cryopreservation of pig embryos. J Reprod Fertil 1997, 52(Suppl.):301-312.
    • (1997) J Reprod Fertil , vol.52 , Issue.SUPPL. , pp. 301-312
    • Dobrinsky, J.R.1
  • 6
    • 56749096477 scopus 로고    scopus 로고
    • Animal oocyte and embryo cryopreservation
    • Pereira R.M., Marques C.C. Animal oocyte and embryo cryopreservation. Cell Tissue Banking 2008, 9:267-277.
    • (2008) Cell Tissue Banking , vol.9 , pp. 267-277
    • Pereira, R.M.1    Marques, C.C.2
  • 7
    • 28044467165 scopus 로고    scopus 로고
    • Modifying oocytes and embryos to improve their cryopreservation
    • Seidel G.E. Modifying oocytes and embryos to improve their cryopreservation. Theriogenology 2006, 65:228-235.
    • (2006) Theriogenology , vol.65 , pp. 228-235
    • Seidel, G.E.1
  • 10
    • 77953961242 scopus 로고    scopus 로고
    • Changes of lipid composition in non-cultured and cultured porcine embryos
    • Romek M., Gajda B., Krzysztofowicz E., Smorag Z. Changes of lipid composition in non-cultured and cultured porcine embryos. Theriogenology 2010, 74:265-276.
    • (2010) Theriogenology , vol.74 , pp. 265-276
    • Romek, M.1    Gajda, B.2    Krzysztofowicz, E.3    Smorag, Z.4
  • 12
    • 0033965247 scopus 로고    scopus 로고
    • Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida
    • Mc Evoy T.G., Coull G.D., Broadbent P.J., Hutchinson J.S.M., Speake B.K. Fatty acid composition of lipids in immature cattle, pig and sheep oocytes with intact zona pellucida. J Reprod Fertil 2000, 118:163-170.
    • (2000) J Reprod Fertil , vol.118 , pp. 163-170
    • Mc Evoy, T.G.1    Coull, G.D.2    Broadbent, P.J.3    Hutchinson, J.S.M.4    Speake, B.K.5
  • 13
    • 0042525896 scopus 로고    scopus 로고
    • Energy metabolism in pig oocytes and early embryos
    • Sturmey R.G., Leese H.J. Energy metabolism in pig oocytes and early embryos. Reproduction 2003, 126:197-204.
    • (2003) Reproduction , vol.126 , pp. 197-204
    • Sturmey, R.G.1    Leese, H.J.2
  • 14
    • 0036163105 scopus 로고    scopus 로고
    • Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro
    • Swain J.E., Bormann C.L., Clark S.G., Walters E.M., Wheeler M.B., Krisher R.L. Use of energy substrates by various stage preimplantation pig embryos produced in vivo and in vitro. Reproduction 2002, 123:253-260.
    • (2002) Reproduction , vol.123 , pp. 253-260
    • Swain, J.E.1    Bormann, C.L.2    Clark, S.G.3    Walters, E.M.4    Wheeler, M.B.5    Krisher, R.L.6
  • 15
    • 33746619170 scopus 로고    scopus 로고
    • A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development
    • Ferguson E.M., Leese H.J. A potential role for triglyceride as an energy source during bovine oocyte maturation and early embryo development. Mol Reprod 2006, 73:1195-1201.
    • (2006) Mol Reprod , vol.73 , pp. 1195-1201
    • Ferguson, E.M.1    Leese, H.J.2
  • 16
    • 69249226015 scopus 로고    scopus 로고
    • Utilization of [U-14C] glucose by preimplantation rat embryo cultured in vitro
    • Tsujii H., Nakamura Y. Utilization of [U-14C] glucose by preimplantation rat embryo cultured in vitro. J Mamm Ova Res 1999, 16:94-97.
    • (1999) J Mamm Ova Res , vol.16 , pp. 94-97
    • Tsujii, H.1    Nakamura, Y.2
  • 17
    • 0036371304 scopus 로고    scopus 로고
    • Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum free or serum-containing media
    • Abe H., Yamashita S., Satoh T., Hoshi H. Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum free or serum-containing media. Mol Reprod Dev 2002, 61:57-66.
    • (2002) Mol Reprod Dev , vol.61 , pp. 57-66
    • Abe, H.1    Yamashita, S.2    Satoh, T.3    Hoshi, H.4
  • 18
    • 0036873960 scopus 로고    scopus 로고
    • Vitrification of cultured and non-cultured expanded and hatched porcine blastocysts
    • Gajda B., Smorag Z. Vitrification of cultured and non-cultured expanded and hatched porcine blastocysts. CryoLett 2002, 23:385-388.
    • (2002) CryoLett , vol.23 , pp. 385-388
    • Gajda, B.1    Smorag, Z.2
  • 19
    • 0033847051 scopus 로고    scopus 로고
    • Survival of pig morula and blastocyst after exposure to vitrification media or vitrification
    • Gajda B., Smorag Z. Survival of pig morula and blastocyst after exposure to vitrification media or vitrification. CryoLett 2000, 21:231-236.
    • (2000) CryoLett , vol.21 , pp. 231-236
    • Gajda, B.1    Smorag, Z.2
  • 20
    • 0021816546 scopus 로고
    • Spectrofluorometric studies of the lipid probe, Nile red
    • Greenspan P., Fowler S.D. Spectrofluorometric studies of the lipid probe, Nile red. J Lipid Res 1985, 26:781-789.
    • (1985) J Lipid Res , vol.26 , pp. 781-789
    • Greenspan, P.1    Fowler, S.D.2
  • 21
    • 31644450228 scopus 로고    scopus 로고
    • A comparative study of cytoplasmic granules imaged by the real-time microscope, Nile Red and Filipin in fibroblasts from patients with lipid storage diseases
    • Pham N.A., Gal M.R., Bagshaw R.D., Mohr A.J., Chue B., Richardson T., Callahan J.W. A comparative study of cytoplasmic granules imaged by the real-time microscope, Nile Red and Filipin in fibroblasts from patients with lipid storage diseases. J Inherit Metab Dis 2005, 28:991-1004.
    • (2005) J Inherit Metab Dis , vol.28 , pp. 991-1004
    • Pham, N.A.1    Gal, M.R.2    Bagshaw, R.D.3    Mohr, A.J.4    Chue, B.5    Richardson, T.6    Callahan, J.W.7
  • 22
    • 13544264648 scopus 로고    scopus 로고
    • Evaluation of the lipid content in bovine oocytes and embryos with Nile red: A practical approach
    • Leroy J.L.M.R., Genicot G., Donnay I., Van Soom A. Evaluation of the lipid content in bovine oocytes and embryos with Nile red: A practical approach. Reprod Dom Anim 2005, 40:76-78.
    • (2005) Reprod Dom Anim , vol.40 , pp. 76-78
    • Leroy, J.L.M.R.1    Genicot, G.2    Donnay, I.3    Van Soom, A.4
  • 23
    • 13544265408 scopus 로고    scopus 로고
    • The use of a fluorescent dye, Nile red, to evaluate the lipid content of single mammalian oocytes
    • Genicot G., Leroy J.L.M.R., Van Soom A., Donnay I. The use of a fluorescent dye, Nile red, to evaluate the lipid content of single mammalian oocytes. Theriogenology 2005, 63:1181-1194.
    • (2005) Theriogenology , vol.63 , pp. 1181-1194
    • Genicot, G.1    Leroy, J.L.M.R.2    Van Soom, A.3    Donnay, I.4
  • 26
    • 0030568183 scopus 로고    scopus 로고
    • Spectroscopic studies of nile red in organic solvent and polymers
    • Dutta A.K., Kamada K., Ohta K. Spectroscopic studies of nile red in organic solvent and polymers. J Photochem Photobiol A: Chem 1996, 93:57-64.
    • (1996) J Photochem Photobiol A: Chem , vol.93 , pp. 57-64
    • Dutta, A.K.1    Kamada, K.2    Ohta, K.3
  • 27
    • 0033625470 scopus 로고    scopus 로고
    • Photophysics of Nile red in solution Steady state spectroscopy
    • Ghoneim N. Photophysics of Nile red in solution Steady state spectroscopy. Spectrochim Acta A 2000, 56:1003-1010.
    • (2000) Spectrochim Acta A , vol.56 , pp. 1003-1010
    • Ghoneim, N.1
  • 28
    • 33845448903 scopus 로고    scopus 로고
    • Membrane localization and dynamics of Nile Red: effect of cholesterol
    • Mukherjee S., Raghuraman H., Chattopadhyay A. Membrane localization and dynamics of Nile Red: effect of cholesterol. Biochim Biophys Acta 2007, 1768:59-66.
    • (2007) Biochim Biophys Acta , vol.1768 , pp. 59-66
    • Mukherjee, S.1    Raghuraman, H.2    Chattopadhyay, A.3
  • 29
    • 70349949073 scopus 로고    scopus 로고
    • Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative
    • Teixeira A.P., Oliveira R., Alves P.M., Carrondo M.J.T. Advances in on-line monitoring and control of mammalian cell cultures: Supporting the PAT initiative. Biotech Adv 2009, 27:726-732.
    • (2009) Biotech Adv , vol.27 , pp. 726-732
    • Teixeira, A.P.1    Oliveira, R.2    Alves, P.M.3    Carrondo, M.J.T.4
  • 31
    • 0028807247 scopus 로고
    • Multivariate curve resolution applied to second order data
    • Tauler R. Multivariate curve resolution applied to second order data. Chemom Intell Lab Sys 1995, 30:133-146.
    • (1995) Chemom Intell Lab Sys , vol.30 , pp. 133-146
    • Tauler, R.1
  • 32
    • 34347254964 scopus 로고    scopus 로고
    • An introduction to multivariate curve resolution-alternating least squares: Spectrophotometric study of the acid-base equilibria of 8-hydroxyquinoline-5-sufonic acid
    • Rdriguez-Rodriguez C., Amigo J.M., Coello J. An introduction to multivariate curve resolution-alternating least squares: Spectrophotometric study of the acid-base equilibria of 8-hydroxyquinoline-5-sufonic acid. J Chem Educ 2007, 84:1190-1192.
    • (2007) J Chem Educ , vol.84 , pp. 1190-1192
    • Rdriguez-Rodriguez, C.1    Amigo, J.M.2    Coello, J.3
  • 33
    • 0035910673 scopus 로고    scopus 로고
    • Criterion for Hill equation validity for description of biosensor calibration curves
    • Kurganov B.I., Lobanov A.V., Borisov I.A., Reshetilov A.N. Criterion for Hill equation validity for description of biosensor calibration curves. Anal Chim Acta 2001, 427:11-19.
    • (2001) Anal Chim Acta , vol.427 , pp. 11-19
    • Kurganov, B.I.1    Lobanov, A.V.2    Borisov, I.A.3    Reshetilov, A.N.4
  • 34
    • 78649863375 scopus 로고    scopus 로고
    • Validation of quantitative fluorescence for determining lipid content of bovine blastocysts stained with nile red dye
    • Barcello-Fimbres M., Seidel G. Validation of quantitative fluorescence for determining lipid content of bovine blastocysts stained with nile red dye. Biol Reprod 2007, 77:87-88.
    • (2007) Biol Reprod , vol.77 , pp. 87-88
    • Barcello-Fimbres, M.1    Seidel, G.2
  • 35
    • 0021923858 scopus 로고
    • Nile red: A selective fluorescent stain for intracellular lipid droplets
    • Greennspan P., Mayer E.P., Fowler D. Nile red: A selective fluorescent stain for intracellular lipid droplets. J Cell Biol 1985, 100:965-973.
    • (1985) J Cell Biol , vol.100 , pp. 965-973
    • Greennspan, P.1    Mayer, E.P.2    Fowler, D.3
  • 37
    • 2342656305 scopus 로고    scopus 로고
    • What does an embryo need
    • Leese H.J. What does an embryo need. Hum Fertil 2003, 6:180-185.
    • (2003) Hum Fertil , vol.6 , pp. 180-185
    • Leese, H.J.1
  • 38
    • 0036856423 scopus 로고    scopus 로고
    • Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro
    • Cetica P., Pintos L., Dalvit G., Beconi M. Activity of key enzymes involved in glucose and triglyceride catabolism during bovine oocyte maturation in vitro. Reproduction 2002, 124:675-681.
    • (2002) Reproduction , vol.124 , pp. 675-681
    • Cetica, P.1    Pintos, L.2    Dalvit, G.3    Beconi, M.4
  • 39
    • 0033653708 scopus 로고    scopus 로고
    • Mitochondrial morphology during preimplantational human embryogenesis
    • Sathananthan A.H., Trounson A.O. Mitochondrial morphology during preimplantational human embryogenesis. Hum Reprod 2000, 15(Suppl. 2):148-159.
    • (2000) Hum Reprod , vol.15 , Issue.SUPPL. 2 , pp. 148-159
    • Sathananthan, A.H.1    Trounson, A.O.2
  • 40
    • 68349163499 scopus 로고    scopus 로고
    • Role of glucose and fatty acid metabolism in porcine early emryo development
    • Sturmey R.G., Leese H.J. role of glucose and fatty acid metabolism in porcine early emryo development. Reprod Fertil Dev 2008, 20:149.
    • (2008) Reprod Fertil Dev , vol.20 , pp. 149
    • Sturmey, R.G.1    Leese, H.J.2
  • 41
    • 0025042845 scopus 로고
    • Participation of glucose in the synthesis of glycoproteins in preimplantation mouse embryos
    • Wales R.G., Hunter J. Participation of glucose in the synthesis of glycoproteins in preimplantation mouse embryos. Reprod Fertil Dev 1990, 2:35-50.
    • (1990) Reprod Fertil Dev , vol.2 , pp. 35-50
    • Wales, R.G.1    Hunter, J.2
  • 42
    • 0027769141 scopus 로고
    • Contribution of the pentose phosphate pathway to glucose utilization by preimplantation sheep embryos
    • Wales R.G., Du Z.F. Contribution of the pentose phosphate pathway to glucose utilization by preimplantation sheep embryos. Reprod Fertil Dev 1993, 5:329-340.
    • (1993) Reprod Fertil Dev , vol.5 , pp. 329-340
    • Wales, R.G.1    Du, Z.F.2
  • 43
    • 0036213162 scopus 로고    scopus 로고
    • REDOX regulation of early embryo development
    • Harvey A.J., Kind K.L., Thompson J.G. REDOX regulation of early embryo development. Reproduction 2002, 123:479-486.
    • (2002) Reproduction , vol.123 , pp. 479-486
    • Harvey, A.J.1    Kind, K.L.2    Thompson, J.G.3
  • 44
    • 0018813388 scopus 로고
    • Phospholipid synthesis in the pre-implantation mouse embryo
    • Pratt H.P.M. Phospholipid synthesis in the pre-implantation mouse embryo. J Reprod Fertil 1993, 58:237-248.
    • (1993) J Reprod Fertil , vol.58 , pp. 237-248
    • Pratt, H.P.M.1
  • 45
    • 0024462282 scopus 로고
    • Organisation and assembly of the surface membrane during early cleavage of the mouse embryo
    • Pratt H.P.M., George M.A. Organisation and assembly of the surface membrane during early cleavage of the mouse embryo. Roux's Archives of Developmental Biology 1989, 198:170-178.
    • (1989) Roux's Archives of Developmental Biology , vol.198 , pp. 170-178
    • Pratt, H.P.M.1    George, M.A.2
  • 46
    • 77956243150 scopus 로고    scopus 로고
    • Oocyte and embryo cryopreservation - State of art and recent developments in domestic animals
    • Gajda B., Smorag Z. Oocyte and embryo cryopreservation - State of art and recent developments in domestic animals. J Anim Feed Sci 2009, 18:371-387.
    • (2009) J Anim Feed Sci , vol.18 , pp. 371-387
    • Gajda, B.1    Smorag, Z.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.