In vitro pancreas organogenesis from dispersed mouse embryonic progenitors

Journal of Visualized Experiments

Volumn , Issue 89, 2014, Pages

  Greggio, Chiara ^a   De Franceschi, Filippo ^a   Figueiredo Larsen, Manuel ^b   Grapin Botton, Anne ^a,b  

^a EPFL   (Switzerland)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

3D culture; Beta cell; Branching epithelium; Cell organization; Development; Developmental biology; Diabetes; Differentiation; Issue 89; Morphogenesis; Organ culture; Pancreas; Progenitors

Indexed keywords

EID: 84904672672     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51725     Document Type: Article

References (29)

1. Pagliuca, F. W., & Melton, D. A. How to make a functional beta-cell. Development. 140, 2472-2483, doi:10.1242/dev.093187 (2013).
2. Percival, A. C., & Slack, J. M. Analysis of pancreatic development using a cell lineage label. Exp Cell Res.247, 123-132, doi:10.1006/excr.1998.4322 (1999).
3. Attali, M. et al. Control of beta-cell differentiation by the pancreatic mesenchyme. Diabetes. 56, 1248-1258, doi:10.2337/db06-1307 (2007).
4. Johansson, K. A. et al. Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types. Dev Cell. 12, 457-465, doi:10.1016/j.devcel.2007.02.010 (2007).
5. Speier, S., & Rupnik, M. A novel approach to in situ characterization of pancreatic beta-cells. Pflugers Arch. 446, 553-558, doi:10.1007/s00424-003-1097-9 (2003).
6. Golosow, N., & Grobstein, C. Epitheliomesenchymal interaction in pancreatic morphogenesis. Dev Biol. 4, 242-255 (1962).
7. Miralles, F., Czernichow, P., & Scharfmann, R. Follistatin regulates the relative proportions of endocrine versus exocrine tissue during pancreatic development. Development. 125, 1017-1024 (1998).
8. Petzold, K. M., & Spagnoli, F. M. A system for ex vivo culturing of embryonic pancreas. Journal of visualized experiments: JoVE. e3979, doi:10.3791/3979 (2012).
9. Miralles, F., Battelino, T., Czernichow, P., & Scharfmann, R. TGF-beta plays a key role in morphogenesis of the pancreatic islets of Langerhans by controlling the activity of the matrix metalloproteinase MMP-2. J Cell Biol. 143, 827-836 (1998).
10. Hope, K., & Bhatia, M. Clonal interrogation of stem cells. Nat Methods. 8, S36-40, doi:10.1038/nmeth.1590 (2011).
11. Sugiyama, T., Rodriguez, R. T., McLean, G. W., & Kim, S. K. Conserved markers of fetal pancreatic epithelium permit prospective isolation of islet progenitor cells by FACS. Proc Natl Acad Sci U S A. 104, 175-180 (2007).
12. Reynolds, B. A., & Weiss, S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science. 255, 1707-1710 (1992).
13. Dontu, G. et al. In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells. Genes Dev. 17, 1253-1270, doi:10.1101/gad.1061803 (2003).
14. Smukler, S. R. et al. The adult mouse and human pancreas contain rare multipotent stem cells that express insulin. Cell Stem Cell. 8, 281-293, doi:S1934-5909(11)00016-6 [pii] 10.1016/j.stem.2011.01.015 (2011).
15. Seaberg, R. M. et al. Clonal identification of multipotent precursors from adult mouse pancreas that generate neural and pancreatic lineages. Nat Biotechnol. 22, 1115-1124 (2004).
16. Jin, L. et al. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel. Proc Natl Acad Sci U S A. 110, 3907-3912, doi:10.1073/pnas.1301889110 (2013).
17. Sugiyama, T. et al. Reconstituting pancreas development from purified progenitor cells reveals genes essential for islet differentiation. Proc Natl Acad Sci U S A. 110, 12691-12696, doi:10.1073/pnas.1304507110 (2013).
18. Huch, M. et al. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. Embo J, doi:10.1038/emboj.2013.204 (2013).
19. Sato, T. et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. (2009).
20. Ootani, A. et al. Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche. Nat Med. 15, 701-706 (2009).
21. Barker, N. et al. Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro. Cell Stem Cell. 6, 25-36, doi:10.1016/j.stem.2009.11.013 (2010).
22. Huch, M. et al. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature. 494, 247-250, doi:10.1038/nature11826 (2013).
23. Lukacs, R. U., Goldstein, A. S., Lawson, D. A., Cheng, D., & Witte, O. N. Isolation, cultivation and characterization of adult murine prostate stem cells. Nat Protoc. 5, 702-713, doi:10.1038/nprot.2010.11 (2010).
24. Rock, J. R. et al. Basal cells as stem cells of the mouse trachea and human airway epithelium. Proc Natl Acad Sci U S A. 106, 12771-12775, doi:10.1073/pnas.0906850106 (2009).
25. Eiraku, M. et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature. 472, 51-56, doi:10.1038/nature09941 (2011).
26. Spence, J. R. et al. Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro. Nature. 470, 105-109, doi:10.1038/nature09691 (2011).
27. Lancaster, M. A. et al. Cerebral organoids model human brain development and microcephaly. Nature, doi:10.1038/nature12517 (2013).
28. Greggio, C. et al. Artificial three-dimensional niches deconstruct pancreas development in vitro. Development. 140, 4452-4462, doi:10.1242/dev.096628 (2013).
29. Muzumdar, M. D., Tasic, B., Miyamichi, K., Li, L., & Luo, L. A global double-fluorescent Cre reporter mouse. Genesis. 45, 593-605, doi:10.1002/dvg.20335 (2007).