Characterization of viral capsid protein self-assembly around short single-stranded RNA

Journal of Physical Chemistry B

Volumn 118, Issue 27, 2014, Pages 7510-7519

  Comas Garcia, Mauricio ^a,c   Garmann, Rees F ^a   Singaram, Surendra W ^a,b   Ben Shaul, Avinoam ^b   Knobler, Charles M ^a   Gelbart, William M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE SPECTROSCOPY; PROTEINS; VIRUSES;

COMMON STRATEGY; COWPEA CHLOROTIC MOTTLE VIRUS; FLUORESCENCE CORRELATION SPECTROSCOPY; LOW CONCENTRATIONS; RELATIVE STRENGTH; RNA MOLECULES; VIRUS ASSEMBLY; VIRUS-LIKE PARTICLES;

RNA;

EID: 84904300248     PISSN: 15206106     EISSN: 15205207     Source Type: Journal    
DOI: 10.1021/jp503050z     Document Type: Article

References (34)

1. Venter, P. A.; Krishna, N. K.; Schneemann, A. Capsid Protein Synthesis From Replicating RNA Directs Specific Packaging Of The Genome Of A Multipartite, Positive-Strand RNA Virus J. Virol. 2005, 79, 6239-6248
2. Bancroft, J. B. The Self-Assembly Of Spherical Plant Viruses Adv. Virus Res. 1970, 16, 99-134
3. Adolph, K. W.; Butler, P. J. G. Studies On The Assembly Of A Spherical Plant Virus. III. Reassembly Of Infectious Virions Under Mild Conditions J. Mol. Biol. 1977, 109, 345-357
4. Annamalai, P.; Rao, A. L. N. Dispensability Of 3′ tRNA-Like Sequence For Packaging Cowpea Chlorotic Mottle Virus Genomic RNAs Virology 2005, 332, 650-658
5. Kobayashi, A.; Ehara, A. In Vitro Encapsidation Of Cucumber Mosaic Virus RNA Species Ann. Phytopathol. Soc. Jpn. 1995, 61, 99-102
6. Ford, R. J.; Barker, A. M.; Bakker, S. E.; Coutts, R. H.; Ranson, N. A.; Phillips, S. E. V.; Person, A. R.; Stockley, P. G. Sequence-Specific, RNA-Protein Interactions Overcome Electrostatic Barriers Preventing Assembly Of Satellite Tobacco Necrosis Virus Coat Protein J. Mol. Biol. 2013, 425, 1050-1064
7. Cadena-Nava, R. D.; Comas-Garcia, M.; Garmann, R. F.; Rao, A. L. N.; Knobler, C. M.; Gelbart, W. M. Self-Assembly Of Viral Capsid Protein And RNA Molecules Of Different Sizes: Requirement For A Specific High Protein/RNA Mass Ratio J. Virol. 2012, 86, 3318-3326
8. Comas-Garcia, M.; Cadena-Nava, R. D.; Rao, A. L. N.; Knobler, C. M.; Gelbart, W. M. In Vitro Quantification Of The Relative Packaging Efficiencies Of Single-Stranded RNA Molecules By Viral Capsid Protein J. Virol. 2012, 86, 12271-12282
9. Garmann, R. F.; Comas-Garcia, M.; Gopal, A.; Knobler, C. M.; Gelbart, W. M. The Assembly Pathway Of An Icosahedral Single-Stranded RNA Virus Depends On The Strength Of Inter-Subunit Attraction J. Mol. Biol. 2014, 426, 1050-1060
10. Rao, A. L. N. Genome Packaging By Spherical Plant RNA Viruses Annu. Rev. Phytopathol. 2006, 44, 61-87
11. Annamalai, P.; Rao, A. L. N. Packaging Of Brome Mosaic Virus Sub- Genomic RNA Is Functionally Coupled To Replication-Dependent Transcription And Translation Of Coat Protein J. Virol. 2006, 80, 10096-10108
12. Venter, P. A.; Schneemann, A. Assembly Of Two Independent Populations Of Flock House Virus Particles With Distinct RNA Packaging Characteristics In The Same Cell J. Virol. 2007, 81, 613-619
13. Fox, J. M.; Wang, G.; Speir, J. A.; Olson, N. H.; Johnson, J. E.; Baker, T. S.; Young, M. J. Comparison Of The Native CCMV Virion With In Vitro Assembled CCMV Virions By Cryoelectron Microscopy And Image Reconstruction Virology 1998, 244, 212-218
14. Johnson, J. M.; Willits, D. A.; Young, M. J.; Zlotnick, A. Interaction With Capsid Protein Alters RNA Structure And The Pathway For In Vitro Assembly Of Cowpea Chlorotic Mottle Virus J. Mol. Biol. 2004, 335, 455-464
15. Dreher, T. W.; Rao, A. L. N.; Hall, T. C. Replication In Vivo Of Mutant Brome Mosaic Virus Rnas Defective In Aminoacylation J. Mol. Biol. 1989, 206, 425-438
16. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular cloning: a laboratory manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, 1989.
17. Bancroft, J. B.; Hills, G. J.; Markham, R. A Study Of The Self-Assembly Process In A Small Spherical Virus Formation Of Organized Structures From Protein Subunits In Vitro Virology 1967, 31, 354-379
18. Gillitzer, E.; Willits, D.; Young, M. J.; Douglas, T. Chemical Modification Of A Viral Cage For Multivalent Presentation Chem. Commun. 2002, 20, 2390-2391
19. Tsay, J. M.; Doose, S.; Weiss, S. Rotational And Translational Diffusion Of Peptide-Coated [1] CdSe/CdS/ZnS Nanorods Studied By Fluorescence Correlation Spectroscopy J. Am. Chem. Soc. 2006, 128, 1639-1647
20. Gopal, A.; Zhou, H. Z.; Knobler, C. M.; Gelbart, W. M. Visualizing Large RNA Molecules In Solution RNA 2012, 18, 284-299
21. Vega-Acosta, J. R.; Cadena-Nava, R. D.; Gelbart, W. M.; Knobler, C. M.; Ruiz- Garcia, J. Electrophoresis Mobilities of a Viral Capsid, Its Capsid Protein, and Their Relation to Viral Assembly J. Phys. Chem. B 2014, 118, 1984-1989
22. Beckett, D.; Wu, H. N.; Uhlenbeck, O. C. Roles Of Operator And Non-Operator RNA Sequences In Bacteriophage R17 Capsid Assembly J. Mol. Biol. 1988, 204, 939-947
23. Borodavka, A.; Tuma, R.; Stockley, P. G. A Two-Stage Mechanism Of Viral RNA Compaction Revealed By Single Molecule Fluorescence RNA Biol. 2013, 10, 1-9
24. Meseth, U.; Wohland, T.; Rigler, R.; Vogel, V. Resolution Of Fluorescence Correlation Measurements Biophys. J. 1999, 76, 1619-1631
25. Zlotnick, A.; Porterfield, J. Z.; Wang, C.-Y. To Build A Virus On A Nucleic Acid Substrate Biophys. J. 2013, 104, 1595-1604
26. Porterfield, J. Z.; Dhason, M. S.; Loeb, D. D.; Nassal, M.; Stray, S. J.; Zlotnick, A. Full-Length Hepatitis B Virus Core Protein Packages Viral And Heterologous RNA With Similarly High Levels Of Cooperativity J. Virol. 2010, 84, 7174-7184
27. Kler, S.; Wang, C.-Y. J.; Ohason, M.; Oppenheim, A.; Zlotnick, A. Scaffold Properties Are A Key Determinant Of The Size And Shape Of Self-Assembled Virus-Derived Particles ACS Chem. Biol. 2013, 8, 2753-2761
28. Hiebert, E.; Bancroft, J. B. Factors Affecting the Assembly of Some Spherical Viruses Virology 1969, 39, 296-311
29. Adolph, K. W.; Butler, P. J. G. Studies On The Assembly Of A Spherical Plant Virus. I. States Of Aggregation Of The Isolated Protein J. Mol. Biol. 1974, 88, 327-338
30. Lavelle, L.; Cadena-Nava, R. D.; Gingery, M.; Vega-Acosta, J. R.; Phillips, M.; Pinedo-Torres, L. A.; Ruiz-Garcia, J.; Gelbart, W. M.; Knobler, C. M. Phase Diagram Of Self-Assembled Viral Capsid Protein Polymorphs J. Phys. Chem. B 2009, 113, 3813-3820
31. Prinsen, P.; van der Schoot, P.; Gelbart, W. M.; Knobler, C. M. Multishell Structures Of Virus Coat Proteins J. Phys. Chem. B 2010, 114, 5522-5533
32. Elrad, O. M.; Hagan, M. F. Encapsulation Of A Polymer By An Icosahedral Virus Phys. Biol. 2010, 7, 045003
33. Devkota, B.; Petrov, A. S.; Lemieux, S.; Boz, M. B.; Tang, L.; Schneemann, A.; Johnson, J. E.; Harvey, S. C. Structural And Electrostatic Characterization Of Pariacoto Virus: Implication For Viral Assembly Biopolymers 2009, 91, 530-538
34. Zhang, R.; Linse, P. Icosahedral capsid formation by capsomers and short polyions J. Chem. Phys. 2013, 138, 1549011-15490114