Microorganisms in respiratory tract of patients diagnosed with atypical pneumonia: Results of a research based on the use of Reverse Transcription Polymerase Chain Reaction (RT-PCR) DNA microarray method and enzyme-linked immunosorbent assay

Clinical Laboratory

Volumn 60, Issue 6, 2014, Pages 1027-1034

  Tokman, Hrisi Bahar ^a   Aslan, Mustafa ^a   Ortaköylü, Gönenç ^b   Algingil, Reyhan Çalişkan ^a   Yüksel, Pelin ^a   Karakullukçu, Asiye ^a   Kalayci, Fatma ^a   Saribaş, Suat ^a   Çakan, Hüseyin ^c   Demir, Tuncalp ^a   Kocazeybek, Bekir S ^a  

^a ISTANBUL UNIVERSITY   (Turkey)

^b Yedikule Chest Diseases and Chest Surgery Training and Research Hospital   (Turkey)

^c ISTANBUL UNIVERSITY   (Turkey)

Author keywords

Atypical pneumonia; CLART pneumovir; RT PCR DNA microarray

Indexed keywords

IMMUNOGLOBULIN M;

ADULT; ARTICLE; BACTERIUM DETECTION; BLOOD SAMPLING; CASE CONTROL STUDY; CHLAMYDOPHILA PNEUMONIAE; CONTROLLED STUDY; CROSS-SECTIONAL STUDY; DIAGNOSTIC KIT; DIAGNOSTIC TEST ACCURACY STUDY; DNA MICROARRAY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; IMMUNOFLUORESCENCE; LEGIONELLA PNEUMOPHILA; MAJOR CLINICAL STUDY; MALE; MYCOPLASMA PNEUMONIAE; NONHUMAN; NOSE SMEAR; PARAINFLUENZA VIRUS 3; PREDICTIVE VALUE; RESPIRATORY VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; VIRUS PNEUMONIA;

ADOLESCENT; ADULT; BACTERIA; CHI-SQUARE DISTRIBUTION; CHILD; CHILD, PRESCHOOL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; INFANT; MALE; NASOPHARYNX; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PNEUMONIA; PREDICTIVE VALUE OF TESTS; REAGENT KITS, DIAGNOSTIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; VIRUSES; YOUNG ADULT;

EID: 84903774787     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: 10.7754/Clin.Lab.2013.130731     Document Type: Article

References (26)

1. Pérez-Ruiz M, Pedrosa-Corral I, Sanbonmatsu-Gámez S, et al. Laboratory detection of respiratory viruses by automated techniques. Open Virol J 2012;6:151-9.
2. Wiemken TL, Peyrani P, Ramirez JA. Global change in the epidemiology of community acquired pneumonia. Semin Respir Crit Care Med 2012;33:213- 9.
3. Dabisch-Ruthe M, Vollmer T, Adams O, Knabbe C, Dreier J. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, Respi Finder 19 assay and Respi Finder SMART 22 assay. BMC Infect Dis 2012;24:163.
4. Shah BA, Ahmed W, Dhobi GN, Shah NN, Khursheed SQ, Haq I. Validity of pneumonia severity index and CURB - 65 severity scoring systems in community acquired pneumonia in an Indian setting. Indian J Chest Dis Allied Sci 2010;52:9-17.
5. Cannon GA, Can MJ, Yandle Z, et al. A low density oligonucleotide microarray for the detection of viral and atypical bacterial respiratory pathogens. J Virol Methods 2010;163:17-24.
6. Choudhary ML, Anand SP, Heydari M, et al. Development of a multiplex one step RT-PCR that detects eighteen respiratory viruses in clinical specimens and comparison with real time RT-PCR. J Virol Methods 2013;189:15-9.
7. De-Paris F, Beck C, Machado AB. Optimization of one-step duplex real-time RT-PCR for detection of influenza and respiratory syncytial virus in nasopharyngeal aspirates. J Virol Methods 2012;186:189-92.
8. Jartti T, Söderlund-Venermo M, Hedman K, Ruuskanen O, Mäkelä MJ. New molecular virus detection methods and their clinical value in lower respiratory tract infections in children. Paediatr Respir Rev 2013;14:38-45.
9. Sakthivel SK, Whitaker B, Lu X, et al. Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses. J Virol Methods 2012;185:259-66.
10. Hegde P, Qi R, Abernathy K, et al. A concise guide to cDNA microarray analysis biotechniques. 2000;29:548-50, 552-54.
11. Frobert E, Escuret V, Javouhey E, et al. Respiratory viruses in children admitted to hospital intensive care units: evaluating the CLART® Pneumovir DNA array. J Med Virol 2011;83:150-5.
12. Renois F, Talmud D, Huguenin A, et al. Rapid detection of respiratory tract viral infections and coinfections in patients with influenza-like illnesses by use of reverse transcription-PCR DNA microarray systems. J Clin Microbiol 2010;48:3836-42.
13. CLART®Pneumovir.(http://www.genomica.es/en/documents/ CLARTPneumoVirV8Nov2010English.pdf),2010.
14. Aleman A, Marcos MA, Pena MJ, et al. Characterization of viruses causing Human Respiratory Infections via Genomic Identification for in vitro diagnosis. Clinical Arrays/CLART PneumoVir, 2008, Abst. P1604, p.464. Abstr.18th ECCMID.
15. Leekha S, Irish C L, Schneider SK, et al. Viral detection using a multiplex polymerase chain reaction-based assay in outpatients with upper respiratory infection. Diagn Microbiol Infect Dis 2013;75:169-73.
16. Chang ML, Jordan-Villegas A, Evans A, et al. Respiratory viruses identified in an urban children's hospital emergency department during the 2009 influenza A(H1N1) pandemic. Pediatr Emerg Care 2012;28:990-7.
17. Gulen F, Cicek C, Kurugol Z, et al. Parainfluenza type 3 outbreaks in Izmir children, Turkey. Trop Doct 2007;37:252-4.
18. Kim JH, Yu JJ, Lee J, et al. Detection rate and clinical impact of respiratory viruses in children with Kawasaki disease. Korean J Pediatr 2012;55:470-3.
19. Luchsinger V, Piedra PA, Ruiz M, et al. Role of Neutralizing Antibodies in Adults With Community-Acquired Pneumonia by Respiratory Syncytial Virus. Clin Infect Dis 2012;54:905-12.
20. Wiemken T, Peyrani P, Bryant K, et al. Incidence of respiratory viruses in patients with community-acquired pneumonia admitted to the intensive care unit: results from the Severe Influenza Pneumonia Surveillance (SIPS) project. Eur J Clin Microbiol Infect Dis 2013;32:705-10.
21. Forman MS, Advani S, Newman C, Gaydos AC, Milstone AM, Valsamakis A. Diagnostic performance of two highly multiplexed respiratory virus assays in a pediatric cohort. J Clin Virol 2012; 55:168-72.
22. Gutierrez F, Masia M, Mirete C, et al. The influence of age and gender on the population-based incidence of community-acquired pneumonia caused by different microbial pathogens. J Infect 2006;53:166.
23. Vervloet LA, Marguet C, Camargos PA. Infection by Mycoplasma pneumoniae and its importance as an etiological agent in childhood community-acquired pneumonias. Braz J Infect Dis 2007;11:507-14. (Pubitemid 350111050)
24. Zer Y, Bayram N, Balci I, Ayten F. Investigation of the causative agents for community-acquired pneumonia in adult patients. Turk J Med Sci 2010;40:47-52.
25. Thurman K A, Walter ND, Schwartz SB, et al. Comparison of laboratory diagnostic procedures for detection of Mycoplasma pneumoniae in community outbreaks. Clin Infect Dis 2009;48: 1244-9.
26. Blyth CC, Webb SA, Kok J, et al. The impact of bacterial and viral co-infection in severe influenza. Influenza Other Respi Viruses 2013;7:168-76.