Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses

Journal of Virological Methods

Volumn 185, Issue 2, 2012, Pages 259-266

  Sakthivel, Senthilkumar K ^a,b   Whitaker, Brett ^a,c   Lu, Xiaoyan ^a   Oliveira, Danielle B L ^a,d   Stockman, Lauren J ^a   Kamili, Shifaq ^a,c   Oberste, M Steven ^a   Erdman, Dean D ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b Logistics Health Incorporated   (United States)

^c ATLANTA RESEARCH AND EDUCATION FOUNDATION   (United States)

^d UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Multiplex; Real time PCR; Respiratory virus

Indexed keywords

ACUTE DISEASE; ADENOVIRUS; ANALYTICAL PARAMETERS; ARTICLE; ASSAY; BOCAVIRUS; CHILD; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ERROR; DIAGNOSTIC TEST ACCURACY STUDY; ENTEROVIRUS; FAST TRACK DIAGNOSTICS RESPIRATORY PATHOGENS ASSAY; HOSPITAL PATIENT; HOSPITALIZATION; HUMAN; INSTRUMENTATION; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MULTIPLEX POLYMERASE CHAIN REACTION; MULTIPLEX REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; RESPIRATORY TRACT DISEASE; RESPIRATORY VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHINOVIRUS; SAMPLING; SENSITIVITY AND SPECIFICITY; SINGLEPLEX REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS CULTURE; VIRUS DETECTION; VIRUS IDENTIFICATION; VIRUS LOAD; VIRUS STRAIN;

ADENOVIRIDAE; ADENOVIRUS INFECTIONS, HUMAN; HUMANS; NASOPHARYNGEAL DISEASES; PICORNAVIRIDAE INFECTIONS; PROSPECTIVE STUDIES; REAGENT KITS, DIAGNOSTIC; REAL-TIME POLYMERASE CHAIN REACTION; RESPIRATORY SYNCYTIAL VIRUS INFECTIONS; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHINOVIRUS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIRAL LOAD;

EID: 84867617945     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2012.07.010     Document Type: Article

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