Development and validation of TOF-SIMS and CLSM imaging method for cytotoxicity study of ZnO nanoparticles in HaCaT cells

Journal of Hazardous Materials

Volumn 277, Issue , 2014, Pages 3-12

  Lee, Pei Ling ^a,c   Chen, Bo Chia ^a   Gollavelli, Ganesh ^a   Shen, Sin Yu ^b   Yin, Yu Sheng ^a   Lei, Shiu Ling ^a   Jhang, Cian Ling ^b   Lee, Woan Ruoh ^b   Ling, Yong Chien ^a,b  

^a NATIONAL TSING HUA UNIVERSITY   (Taiwan)

^b TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^c INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE   (Taiwan)

Author keywords

HaCaT cells; Nanotoxicity; Stable isotope labeled; TOF SIMS; ZnO nanoparticles

Indexed keywords

CELL DEATH; CELL MEMBRANES; CHEMICAL ANALYSIS; CYTOTOXICITY; DISSOLUTION; ISOTOPES; METAL NANOPARTICLES; SECONDARY ION MASS SPECTROMETRY;

CONFOCAL LASER SCANNING MICROSCOPY IMAGING; NANOTOXICITY; PHYSIO-CHEMICAL PROPERTIES; STABLE ISOTOPE-LABELED; TIME OF FLIGHT SECONDARY ION MASS SPECTROMETRY; TOF SIMS; ZINC OXIDE NANOPARTICLES; ZNO NANOPARTICLES;

ZINC OXIDE;

CALCIUM ION; GLUTATHIONE; PHOSPHORYLCHOLINE; POTASSIUM ION; STABLE ISOTOPE; ZINC OXIDE NANOPARTICLE; DRUG CARRIER; NANOPARTICLE; ZINC; ZINC OXIDE;

ADSORPTION; AGGREGATION; AQUEOUS SOLUTION; CYTOPLASM; DISSOLUTION; MEMBRANE; NANOTECHNOLOGY; PHYSICOCHEMICAL PROPERTY; SKIN; TOXICITY TEST; ZINC;

ABSORPTION; AQUEOUS SOLUTION; ARTICLE; CALCIUM CELL LEVEL; CELL AGGREGATION; CELL FUNCTION; CELL NUCLEUS; CELL SHAPE; CELL SIZE; CELL SURVIVAL; CELL VIABILITY; CELLULAR DISTRIBUTION; COMPARATIVE STUDY; CONCENTRATION (PARAMETERS); CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; CORRELATIONAL STUDY; CRYSTAL STRUCTURE; CYTOPLASM; CYTOTOXICITY; CYTOTOXICITY TEST; DISSOLUTION; HUMAN; HUMAN CELL; ISOTOPE LABELING; KERATINOCYTE; PHYSICAL CHEMISTRY; POTASSIUM CELL LEVEL; SURVIVAL RATE; TIME OF FLIGHT MASS SPECTROMETRY; VALIDATION STUDY; CELL LINE; CHEMISTRY; CONFOCAL MICROSCOPY; CYTOLOGY; DEVICES; DOSE RESPONSE; DRUG EFFECTS; MASS SPECTROMETRY; PROCEDURES; REPRODUCIBILITY; SKIN; SOLUBILITY; SURFACE PROPERTY; TOXICITY; TOXICITY TESTING;

CELL LINE; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG CARRIERS; HUMANS; MICROSCOPY, CONFOCAL; NANOPARTICLES; REPRODUCIBILITY OF RESULTS; SKIN; SOLUBILITY; SPECTROMETRY, MASS, SECONDARY ION; SURFACE PROPERTIES; TOXICITY TESTS; ZINC ISOTOPES; ZINC OXIDE;

EID: 84903763651     PISSN: 03043894     EISSN: 18733336     Source Type: Journal    
DOI: 10.1016/j.jhazmat.2014.03.046     Document Type: Article

References (49)

1. Oberdorster G., Oberdorster E., Oberdorster J. Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ. Health Perspect. 2005, 113:823-839.
2. Arora S., Rajwade J.M., Paknikar K.M. Nanotoxicology and in vitro studies: the need of the hour. Toxicol. Applied Pharm. 2012, 258:151-165.
3. Hackenberg S., Kleinsasser N. Dermal toxicity of ZnO nanoparticles: a worrying feature of sunscreen?. Nanomedicine 2012, 7:461-463.
4. Monteiro-Riviere N.A., Wiench K., Landsiedel R., Schulte S., Inman A.O., Riviere J.E. Safety evaluation of sunscreen formulations containing titanium dioxide and zinc oxide nanoparticles in UVB sunburned skin: an in vitro and in vivo study. Toxicol. Sci. 2011, 123:264-280.
5. Gulson B., McCall M., Korsch M., Gomez L., Casey P., Oytam Y., Taylor A., McCulloch M., Trotter J., Kinsley L., Greenoak G. Small amounts of zinc from zinc oxide particles in sunscreens applied outdoors are absorbed through human skin. Toxicol. Sci. 2010, 118:140-149.
6. Standardization mandate assigned to CEN concerning methods for testing efficacy of sunscreen products. EU/EC M389-2006. EC, Brussels, EU.
7. O'Neil M.J. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals 2001, 1811. Merck, Rahway, NJ. 13th ed.
8. Ghule K., Ghule A.V., Chen B.-J., Ling Y.-C. Preparation and characterization of ZnO nanoparticles coated paper and its antibacterial activity study. Green Chem. 2006, 8:1034-1041.
9. Lo B., Chang J.-Y., Ghule A.V., Tzing S.-H., Ling Y.-C. Seed-mediated fabrication of ZnO nanorods with controllable morphology and photoluminescence properties. Scripta Mater. 2006, 54:411-415.
10. Burnett M.E., Wang S.Q. Current sunscreen controversies: a critical review. Photodermatol. Photoimmunol. Photomed. 2011, 27:58-67.
11. DeLouise L.A. Applications of nanotechnology in dermatology. J. Invest. Dermatol. 2012, 132:964-975.
12. Leite-Silva V.R., Lamer M.L., Sanchez W.Y., Liu D.C., Sanchez W.H., Morrow I., Martin D., Silva H.D.T., Prow T.W., Grice J.E., Roberts M.S. The effect of formulation on the penetration of coated and uncoated zinc oxide nanoparticles into the viable epidermis of human skin in vivo. Eur. J. Pharm. Biopharm. 2013, 84:297-308.
13. Bae H., Ryu H., Jeong S., Lee E., Park Y.-H., Lee K., Choi B., Maeng E., Kim M.-K., Son S. Oxidative stress and apoptosis induced by ZnO nanoparticles in HaCaT cells. Mol. Cell. Toxicol. 2011, 7:333-337.
14. Moos P.J., Olszewski K., Honeggar M., Cassidy P., Leachman S., Woessner D., Cutler N.S., Veranth J.M. Responses of human cells to ZnO nanoparticles: a gene transcription study. Metallomics 2011, 3:1199-1211.
15. Chandra S., Smith D.R., Morrison G.H. Peer reviewed: a subcellular imaging by dynamic SIMS ion microscopy. Anal. Chem. 2000, 72:104 A-114 A.
16. 2 and ZnO nanomaterials in commercial sunscreens. J. Nanopart. Res. 2011, 13:3607-3617.
17. Wang J., Hansen E.H. Interfacing sequential injection on-line preconcentration using a renewable micro-column incorporated in a 'lab-on-valve' system with direct injection nebulization inductively coupled plasma mass spectrometry. J. Anal. Atom. Spectrom. 2001, 16:1349-1355.
18. Larner F., Dogra Y., Dybowska A., Fabrega J., Stolpe B., Bridgestock L.J., Goodhead R., Weiss D.J., Moger J., Lead J.R., Valsami-Jones E., Tyler C.R., Galloway T.S., Rehkämper M. Tracing bioavailability of ZnO Nanoparticles using stable isotope labeling. Environ. Sci. Technol. 2012, 46:12137-12145.
19. Mosetlha K., Torto N., Wibetoe G. Determination of Cu and Ni in plants by microdialysis sampling: comparison of dialyzable metal fractions with total metal content. Talanta 2007, 71:766-770.
20. Boukamp P., Petrussevska R.T., Breitkreutz D., Hornung J., Markham A., Fusenig N.E. Normal keratinization in a spontaneously immortalized aneuploidy human keratinocyte cell-line. J. Cell Biol. 1988, 106:761-771.
21. Boelsma E., Verhoeven M.C., Ponec M. Reconstruction of a human skin equivalent using a spontaneously transformed keratinocyte cell line (HaCaT). J. Invest. Dermatol. 1999, 112:489-498.
22. Kanade K.G., Kale B.B., Aiyer R.C., Das B.K. Effect of solvents on the synthesis of nano-size zinc oxide and its properties. Mater. Res. Bull. 2006, 41:590-600.
23. Zhou J., Xu N.S., Wang Z.L. Dissolving behavior and stability of ZnO wires in biofluids: a study on biodegradability and biocompatibility of ZnO nanostructures. Adv. Mater. 2006, 18:2432-2435.
24. Xia T., Kovochich M., Liong M., Madler L., Gilbert B., Shi H.B., Yeh J.I., Zink J.I., Nel A.E. Comparison of the mechanism of toxicity of zinc oxide and cerium oxide nanoparticles based on dissolution and oxidative stress properties. ACS Nano 2008, 2:2121-2134.
25. Sayes C.M., Wahi R., Kurian P.A., Liu Y.P., West J.L., Ausman K.D., Warheit D.B., Colvin V.L. Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. Toxicol. Sci. 2006, 92:174-185.
26. Brayner R. The toxicological impact of nanoparticles. Nano Today 2008, 3:48-55.
27. Yao K.A., Wang N., Zhuang J.Y., Yang Z.B., Ni H.Y., Xu Q., Sun C., Bi S.P. Studies on the effects of Al(III) on the lactate dehydrogenase activity by differential pulse voltammetry. Talanta 2007, 73:529-533.
28. Lin W.S., Huang Y.W., Zhou X.D., Ma Y.F. In vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicol. Appl. Pharmacol. 2006, 217:252-259.
29. Gollavelli G., Ling Y.C. Multi-functional graphene as an in vitro and in vivo imaging probe. Biomaterials 2012, 33:2532-2545.
30. Torto N., Mogopodi D. Opportunities in microdialysis sampling of metal ions. TrAC-Trends Anal. Chem. 2004, 23:109-115.
31. Sjovall P., Lausmaa J., Nygren H., Carlsson L., Malmberg P. Imaging of membrane lipids in single cells by imprint-imaging time-of-flight secondary ion mass spectrometry. Anal. Chem. 2003, 75:3429-3434.
32. Kumar S., Venkateswarlu P., Rao V., Rao G. Synthesis, characterization and optical properties of zinc oxide nanoparticles. Int. Nano Lett. 2013, 3:1-6.
33. Dechsakulthorn F., Hayes A., Bakand S., Joeng L., Winder C. In vitro cytotoxicity assessment of selected nanoparticles using human skin fibroblasts. AATEX 2008, 14:397-400.
34. Karlsson H.L., Cronholm P., Gustafsson J., Moller L. Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes. Chem. Res. Toxicol. 2008, 21:1726-1732.
35. Alkilany A., Murphy C. Toxicity and cellular uptake of gold nanoparticles: what we have learned so far?. J. Nanopart. Res. 2010, 12:2313-2333.
36. Gojova A., Guo B., Kota R.S., Rutledge J.C., Kennedy I.M., Barakat A.I. Induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: effect of particle composition. Environ. Health Perspect. 2007, 115:403-409.
37. Tsou T.-C., Yeh S.-C., Tsai F.-Y., Lin H.-J., Cheng T.-J., Chao H.-R., Tai L.-A. Zinc oxide particles induce inflammatory responses in vascular endothelial cells via NF-κB signaling. J. Hazard. Mater. 2010, 183:182-188.
38. Nel A., Xia T., Madler L., Li N. Toxic potential of materials at the nanolevel. Science 2006, 311:622-627.
39. 2 to a freshwater microalga (Pseudokirchneriella subcapitata): the importance of particle solubility. Environ. Sci. Technol. 2007, 41:8484-8490.
40. Daniels W.M.U., Hendricks J., Salie R., van Rensburg S.J. A mechanism for zinc toxicity in neuroblastoma cells. Metab. Brain Dis. 2004, 19:79-88.
41. Formigare A., Irato P., Santon A. Zinc antioxidant systems and metallothionein in metal mediated-apoptosis: biochemical and cytochemical aspects. Comp. Biochem. Physiol. C: Pharmacol. Toxicol. Endocrinol. 2007, 146:443-459.
42. Frazzini V., Rockabrand E., Mocchegiani E., Sensi S.L. Oxidative stress and brain aging: is zinc the link?. Biogerontology 2006, 7:307-314.
43. Kroll A., Pillukat M.H., Hahn D., Schnekenburger J. Current in vitro methods in nanoparticle risk assessment: limitations and challenges. Eur. J. Pharm. Biopharm. 2009, 72:370-377.
44. Yu K.-N., Yoon T.-J., Minai-Tehrani A., Kim J.-E., Park S.J., Jeong M.S., Ha S.-W., Lee J.-K., Kim J.S., Cho M.-H. Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. Toxicol. In Vitro 2013, 27:1187-1195.
45. Borner K., Malmberg P., Mansson J.E., Nygren H. Molecular imaging of lipids in cells and tissues. Int. J. Mass Spectrom. 2007, 260:128-136.
46. Loewy A.G., Siekevitz P., Menninger J.R., Gallant J.A.N. Cell Structure & Function: An Integrated Approach 1991, Saunders College Publishers, Philadelphia, PA.
47. Arcangeli A., Becchetti A. Complex functional interaction between integrin receptors and ion channels. Trends Cell Biol. 2006, 16:631-639.
48. 2+-activated ion channels in human keratinocytes. FASEB J. 2001, 15:145-154.
49. Chandra S. Quantitative imaging of chemical composition in single cells by secondary ion mass spectrometry: cisplatin affects calcium stores in renal epithelial cells. Methods Mol. Biol. 2010, 656:113-130.