A modified Janus cassette (sweet Janus) to improve allelic replacement efficiency by high-stringency negative selection in Streptococcus pneumoniae

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Li, Yuan ^a   Thompson, Claudette M ^a   Lipsitch, Marc ^a  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; JANUS; KANAMYCIN; STREPTOMYCIN; SUCROSE; SWEET JANUS; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; BACTERIAL STRAIN; DNA MODIFICATION; GENE CASSETTE; GENE FREQUENCY; GENE FUNCTION; NONHUMAN; NUCLEOTIDE SEQUENCE; REVERTANT; SACB GENE; SENSITIVITY ANALYSIS; STREPTOCOCCUS PNEUMONIAE; BACTERIUM TRANSFORMATION; GENE ORDER; GENETIC RECOMBINATION; GENETIC SELECTION; GENETICS; PLASMID; QUANTITATIVE TRAIT LOCUS;

ALLELES; GENE ORDER; PLASMIDS; QUANTITATIVE TRAIT LOCI; RECOMBINATION, GENETIC; SELECTION, GENETIC; STREPTOCOCCUS PNEUMONIAE; TRANSFORMATION, BACTERIAL;

EID: 84903552084     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0100510     Document Type: Article

References (14)

1. Sung CK, Li H, Claverys JP, Morrison DA (2001) An rpsL cassette, janus, for gene replacement through negative selection in Streptococcus pneumoniae. Appl Environ Microbiol 67: 5190-5196. (Pubitemid 33701288)
2. Trzcinski K, Thompson CM, Lipsitch M (2003) Construction of otherwise isogenic serotype 6B, 7F, 14, and 19F capsular variants of Streptococcus pneumoniae strain TIGR4. Appl Environ Microbiol 69: 7364-7370. (Pubitemid 38585389)
3. Li Y, Gierahn T, Thompson CM, Trzcinski K, Ford CB, et al. (2012) Distinct effects on diversifying selection by two mechanisms of immunity against Streptococcus pneumoniae. PLoS Pathog 8: e1002989.
4. Li Y, Weinberger DM, Thompson CM, Trzcinski K, Lipsitch M (2013) Surface Charge of Streptococcus pneumoniae Predicts Serotype Distribution. Infect Immun 81: 4519-4524.
5. Joloba ML, Kidenya BR, Kateete DP, Katabazi FA, Muwanguzi JK, et al. (2010) Comparison of transformation frequencies among selected Streptococcus pneumoniae serotypes. Int J Antimicrob Agents 36: 124-128.
6. Evans BA, Rozen DE (2013) Significant variation in transformation frequency in Streptococcus pneumoniae. ISME J 7: 791-799.
7. Steinmetz M, Le Coq D, Djemia HB, Gay P (1983) [Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg]. Mol Gen Genet 191: 138-144.
8. Dedonder R (1966) Levansucrase from bacillus subtilis. Methods Enzymol 8: 6.
9. Reyrat JM, Pelicic V, Gicquel B, Rappuoli R (1998) Counterselectable markers: untapped tools for bacterial genetics and pathogenesis. Infect Immun 66: 4011-4017. (Pubitemid 28402664)
10. Sun X, Yang D, Wang Y, Geng H, He X, et al. (2013) Development of a markerless gene deletion system for Streptococcus zooepidemicus: functional characterization of hyaluronan synthase gene. Appl Microbiol Biotechnol 97: 8629-8636.
11. Jager W, Schafer A, Puhler A, Labes G, Wohlleben W (1992) Expression of the Bacillus subtilis sacB gene leads to sucrose sensitivity in the gram-positive bacterium Corynebacterium glutamicum but not in Streptomyces lividans. J Bacteriol 174: 5462-5465.
12. Pelicic V, Reyrat JM, Gicquel B (1996) Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria. J Bacteriol 178: 1197-1199. (Pubitemid 26048040)
13. Pozzi G, Masala L, Iannelli F, Manganelli R, Havarstein LS, et al. (1996) Competence for genetic transformation in encapsulated strains of Streptococcus pneumoniae: two allelic variants of the peptide pheromone. J Bacteriol 178: 6087-6090. (Pubitemid 26337802)
14. Trzcinski K, Thompson CM, Srivastava A, Basset A, Malley R, et al. (2008) Protection against nasopharyngeal colonization by Streptococcus pneumoniae is mediated by antigen-specific CD4+ T cells. Infect Immun 76: 2678-2684.