Efficient generation of integration-free human induced pluripotent stem cells from keratinocytes by simple transfection of episomal vectors

Stem Cells Translational Medicine

Volumn 3, Issue 7, 2014, Pages 787-791

  Piao, Yulan ^a   Hung, Sandy Shen Chi ^a,b   Lim, Shiang Y ^b   Wong, Raymond Ching Bong ^a,b   Ko, Minoru S H ^a,c  

^a NATIONAL INSTITUTE ON AGING   (United States)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Derivation of human induced pluripotent stem cells; Episomal vectors; Integration free; Keratinocytes; Lipid mediated transfection

Indexed keywords

BETA ACTIN; GREEN FLUORESCENT PROTEIN; KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; PROTEIN P53; REX PROTEIN; TRANSCRIPTION FACTOR NANOG; TRANSCRIPTION FACTOR SOX2;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; EMBRYOID BODY; FIBROBLAST; GENETIC TRANSFECTION; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; LIPOSOMAL GENE DELIVERY SYSTEM; NONHUMAN; NUCLEAR REPROGRAMMING; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

DERIVATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS; EPISOMAL VECTORS; INTEGRATION-FREE; KERATINOCYTES; LIPID MEDIATED TRANSFECTION;

ANIMALS; CELL DIFFERENTIATION; COCULTURE TECHNIQUES; EMBRYOID BODIES; FEEDER CELLS; FIBROBLASTS; GENE EXPRESSION REGULATION, DEVELOPMENTAL; GENETIC VECTORS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; KERATINOCYTES; MICE; NUCLEAR REPROGRAMMING; PLASMIDS; TERATOMA; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 84903550641     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2013-0036     Document Type: Article

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