Annexation of a high-activity enzyme in a synthetic three-enzyme complex greatly decreases the degree of substrate channeling

ACS Synthetic Biology

Volumn 3, Issue 6, 2014, Pages 380-386

  You, Chun ^a   Zhang, Y H Percival ^a,b,c  

^a Virginia Polytechnic Institute and State University   (United States)

^b Cell Free Bioinnovations Inc   (United States)

^c Gate Fuels Inc   (United States)

Author keywords

cell free synthetic biology; enzyme cascade; metabolon; rate limiting step; substrate channeling; synthetic enzyme complex

Indexed keywords

FRUCTOSE BISPHOSPHATASE; FRUCTOSE BISPHOSPHATE ALDOLASE; MULTIENZYME COMPLEX; TRIOSEPHOSPHATE ISOMERASE; BACTERIAL DNA; CELL CYCLE PROTEIN; COHESIN; NONHISTONE PROTEIN; RECOMBINANT PROTEIN; SYNTHETIC DNA;

ARTICLE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; NONHUMAN; PRIORITY JOURNAL; SYNTHETIC BIOLOGY; THERMOTOGA MARITIMA; THERMUS THERMOPHILUS; CHEMISTRY; DNA FRAGMENTATION; DNA SEQUENCE; ESCHERICHIA COLI; GENETICS; PLASMID; PROTEIN CONFORMATION; PROTEIN SYNTHESIS;

CELL CYCLE PROTEINS; CHROMOSOMAL PROTEINS, NON-HISTONE; DNA FRAGMENTATION; DNA, BACTERIAL; ESCHERICHIA COLI; FRUCTOSE-BISPHOSPHATE ALDOLASE; GENES, SYNTHETIC; MULTIENZYME COMPLEXES; PLASMIDS; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TRIOSE-PHOSPHATE ISOMERASE;

EID: 84903161298     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb4000993     Document Type: Article

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