Non-Complexed Four Cascade Enzyme Mixture: Simple Purification and Synergetic Co-stabilization

PLoS ONE

Volumn 8, Issue 4, 2013, Pages

  Myung, Suwan ^a,b   Zhang, Y H Percival ^a,b,c,d  

^a Virginia Polytechnic Institute and State University   (United States)

^b VIRGINIA POLYTECHNIC INSTITUTE AND STATE UNIVERSITY   (United States)

^c Cell Free Bioinnovations Inc   (United States)

^d Gate Fuels Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIUM SULFATE; BOVINE SERUM ALBUMIN; DIHYDROXYACETONE PHOSPHATE; FRUCTOSE 1,6 BISPHOSPHATE; FRUCTOSE 6 PHOSPHATE; FRUCTOSE BISPHOSPHATASE; FRUCTOSE BISPHOSPHATE ALDOLASE; GLUCOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE ISOMERASE; GLYCERALDEHYDE 3 PHOSPHATE; PENTOSE PHOSPHATE; RECOMBINANT ENZYME; TRIOSEPHOSPHATE ISOMERASE;

ALDOL REACTION; ARTICLE; CLOSTRIDIUM THERMOCELLUM; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; GLUCONEOGENESIS; HALF LIFE TIME; IN VITRO STUDY; MACROMOLECULE; NONHUMAN; PRECIPITATION; THERMOTOGA MARITIMA; THERMUS THERMOPHILUS; TURNOVER TIME;

BACTERIAL PROTEINS; BIOCATALYSIS; CLOSTRIDIUM THERMOCELLUM; ENZYME ASSAYS; ENZYME STABILITY; FRUCTOSE-BISPHOSPHATASE; FRUCTOSE-BISPHOSPHATE ALDOLASE; GLUCOSE-6-PHOSPHATE ISOMERASE; HALF-LIFE; KINETICS; SERUM ALBUMIN, BOVINE; TEMPERATURE; THERMOTOGA MARITIMA; THERMUS THERMOPHILUS; TRIOSE-PHOSPHATE ISOMERASE;

BOVINAE; CLOSTRIDIUM THERMOCELLUM; THERMOTOGA MARITIMA; THERMUS;

EID: 84876019828     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0061500     Document Type: Article

References (37)

1. Benner SA, Sismour AM, (2005) Synthetic biology. Nat Rev Genet 6: 533-543.
2. Zhang Y-HP, (2010) Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: Challenges and opportunities. Biotechnol Bioeng 105: 663-677.
3. Forster AC, Church GM, (2007) Synthetic biology projects in vitro. Genome Res 17: 1-6.
4. Meyer A, Pellaux R, Panke S, (2007) Bioengineering novel in vitro metabolic pathways using synthetic biology. Curr Opin Microbiol 10: 246-253.
5. Zhang Y-HP, Sun J-B, Zhong J-J, (2010) Biofuel production by in vitro synthetic pathway transformation. Curr Opin Biotechnol 21: 663-669.
6. Zhang Y-HP, Evans BR, Mielenz JR, Hopkins RC, Adams MWW, (2007) High-yield hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS One 2: e456.
7. Ye X, Wang Y, Hopkins RC, Adams MWW, Evans BR, et al. (2009) Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails. ChemSusChem 2: 149-152.
8. del Campo JSM, Rollin J, Myung S, Chun Y, Chandrayan S, et al. (2013) High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System. Angew Chem Int Ed: DOI: 10.1002/ange.201300766.
9. Guterl J-K, Garbe D, Carsten J, Steffler F, Sommer B, et al. (2012) Cell-free metabolic engineering- Production of chemicals via minimized reaction cascades. ChemSusChem 5: 2165-2172.
10. Ye X, Honda K, Sakai T, Okano K, Omasa T, et al. (2012) Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway. Microb Cell Fact 11: 120.
11. Ye X, Honda K, Morimoto Y, Okano K, Ohtake H, (2013) Direct conversion of glucose to malate by synthetic metabolic engineering. J Biotechnol 164: 34-40.
12. Wang Y, Huang W, Sathitsuksanoh N, Zhu Z, Zhang Y-HP, (2011) Biohydrogenation from biomass sugar mediated by in vitro synthetic enzymatic pathways. Chem Biol 18: 372-380.
13. Swartz JR, (2011) Transforming biochemical engineering with cell-free biology. AIChE J 58: 5-13.
14. Tong XD, El-Zahab B, Zhao XY, Liu YY, Wang P, (2011) Enzymatic Synthesis of L-Lactic Acid From Carbon Dioxide and Ethanol With an Inherent Cofactor Regeneration Cycle. Biotechnol Bioeng 108: 465-469.
15. Zhang Y-HP, Huang W-D, (2012) Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolution. Trends Biotechnol 30: 301-306.
16. Zhu ZG, Sun F, Zhang X, Zhang Y-HP, (2012) Deep oxidation of glucose in enzymatic fuel cells through a synthetic enzymatic pathway containing a cascade of two thermostable dehydrogenases. Biosens Bioelectron 36: 110-115.
17. Xu S, Minteer SD, (2011) Enzymatic Biofuel Cell for Oxidation of Glucose to CO2. ACS Catal 1: 91-94.
18. Adams MWW, Kelly RM, (1998) Finding and using hyperthermophilic enzymes. Trends Biotechnol 16: 329-332.
19. Wang Y, Zhang YHP, (2010) A highly active phosphoglucomutase from Clostridium thermocellum: cloning, purification, characterization and enhanced thermostability. J Appl Microbiol 108: 39-46.
20. Sun FF, Zhang XZ, Myung S, Zhang YHP, (2012) Thermophilic Thermotoga maritima ribose-5-phosphate isomerase RpiB: Optimized heat treatment purification and basic characterization. Protein Expres Purif 82: 302-307.
21. Wang Y, Zhang Y-HP, (2009) Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration. Microb Cell Fact 8: 30.
22. Myung S, Wang Y, Zhang YHP, (2010) Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: Characterization, metabolite stability, and its implications. Process Biochem 45: 1882-1887.
23. Iyer RB, Wang JQ, Bachas LG, (2002) Cloning, expression, and characterization of the gsdA gene encoding thermophilic glucose-6-phosphate dehydrogenase from Aquifex aeolicus. Extremophiles 6: 283-289.
24. You C, Zhang Y-HP, (2013) Self-assembly of synthetic metabolons through synthetic protein scaffolds: one-step purification, co-immobilization, and substrate channeling. ACS Syn Biol 2: 102-110.
25. Banki MR, Feng L, Wood DW, (2005) Simple bioseparations using self-cleaving elastin-like polypeptide tags. Nat Methods 2: 659-662.
26. Myung S, Zhang XZ, Zhang YHP, (2011) Ultra-Stable Phosphoglucose Isomerase Through Immobilization of Cellulose-Binding Module-Tagged Thermophilic Enzyme on Low-Cost High-Capacity Cellulosic Adsorbent. Biotechnol Progr 27: 969-975.
27. Zhang Y-HP, (2011) Substrate channeling and enzyme complexes for biotechnological applications. Biotechnol Adv 29: 715-725.
28. Ellis RJ, (2001) Macromolecular crowding: obvious but underappreciated. Trends Biochem Sci 26: 597-604.
29. Zhang Y-HP, Myung S, You C, Zhu ZG, Rollin J, (2011) Toward low-cost biomanufacturing through cell-free synthetic biology: bottom-up design. J Mater Chem 21: 18877-18886.
30. Huang SY, Zhang Y-HP, Zhong JJ, (2012) A thermostable recombinant transaldolase with high activity over a broad pH range. Appl Microbiol Biotechnol 93: 2403-2410.
31. You C, Myung S, Zhang Y-HP, (2012) Facilitated substrate channeling in a self-assembled trifunctional enzyme complex. Angew Chem Int Ed 51: 8787-8790.
32. Chang BS, Mahoney RR, (1995) Enzyme Thermostabilization by Bovine Serum-Albumin and Other Proteins- Evidence for Hydrophobic Interactions. Biotechnol Appl Bioc 22: 203-214.
33. Gouda MD, Kumar MA, Thakur MS, Karanth NG, (2002) Enhancement of operational stability of an enzyme biosensor for glucose and sucrose using protein based stabilizing agents. Biosens Bioelectron 17: 503-507.
34. Bruggink A, Schoevaart R, Kieboom T, (2003) Concepts of nature in organic synthesis: Cascade catalysis and multistep conversions in concert. Org Process Res Dev 7: 622-640.
35. Cao L, Langen Lv, Sheldon RA, (2003) Immobilised enzymes: carrier-bound or carrier-free? Curr Opin Biotechnol 14: 387-394.
36. Shuler M, Kargi F (2001) Bioprocess Engineering: Basic Concepts (2nd Ed.). Englewood Cliffs, NJ: Prentice-Hall.
37. Rogers TA, Bommarius AS, (2010) Utilizing simple biochemical measurements to predict lifetime output of biocatalysts in continuous isothermal processes. Chem Eng Sci 65: 2118-2124.