Versatile O-GlcNAc transferase assay for high-throughput identification of enzyme variants, substrates, and inhibitors

Bioconjugate Chemistry

Volumn 25, Issue 6, 2014, Pages 1025-1030

  Kim, Eun J ^a   Abramowitz, Lara K ^b   Bond, Michelle R ^b   Love, Dona C ^b   Kang, Dong W ^c   Leucke, Hans F ^b   Kang, Dae W ^d   Ahn, Jong Seog ^e   Hanover, John A ^b  

^a Daegu University   (South Korea)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c Catholic University of Daegu   (South Korea)

^d Changwon National University   (South Korea)

^e Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; THROUGHPUT;

CELLULAR PROCESS; CELLULARS; GLYCOSYLATIONS; HIGH-THROUGHPUT; IDENTIFICATION OF ENZYMES; N-ACETYLGLUCOSAMINE; NUCLEOCYTOPLASMIC PROTEINS; RAPID METHOD; SIMPLE METHOD; THREONINE RESIDUES;

ENZYMES;

BACTERIUM LYSATE; ENZYME INHIBITOR; ENZYME VARIANT; HISTIDINE; N ACETYLGLUCOSAMINE; SUGAR; BETA N ACETYLHEXOSAMINIDASE; N ACETYLGLUCOSAMINYLTRANSFERASE; NICKEL; O-GLCNAC TRANSFERASE; ORGANOMETALLIC COMPOUND;

ACYLATION; ARTICLE; CHEMICAL REACTION; DRUG DEVELOPMENT; DRUG IDENTIFICATION; DRUG POTENCY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE; EUKARYOTIC CELL; HIGH THROUGHPUT SCREENING; ANTAGONISTS AND INHIBITORS; CHEMICAL STRUCTURE; CHEMISTRY; DOSE RESPONSE; DRUG EFFECTS; ENZYME SPECIFICITY; HUMAN; METABOLISM; STRUCTURE ACTIVITY RELATION;

BETA-N-ACETYLHEXOSAMINIDASES; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ASSAYS; ENZYME INHIBITORS; HUMANS; MOLECULAR STRUCTURE; N-ACETYLGLUCOSAMINYLTRANSFERASES; NICKEL; ORGANOMETALLIC COMPOUNDS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY;

EID: 84902677257     PISSN: 10431802     EISSN: 15204812     Source Type: Journal    
DOI: 10.1021/bc5001774     Document Type: Article

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