Transmission electron microscopy as a tool for nanocrystal characterization pre- and post-injector

Philosophical Transactions of the Royal Society B: Biological Sciences

Volumn 369, Issue 1647, 2014, Pages

  Stevenson, H P ^a   DePonte, D P ^b   Makhov, A M ^a   Conway, James F ^a   Zeldin, O B ^b   Boutet, S ^b   Calero, G ^a   Cohen, A E ^c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

^c SLAC NATIONAL ACCELERATOR LABORATORY   (United States)

Author keywords

Crystal characterization; Crystal injector; Crystallography; Femtosecond diffraction; Sample delivery; Transmission electron microscopy (TEM)

Indexed keywords

CRYSTAL STRUCTURE; FOURIER TRANSFORM; LIGHT EFFECT; PROTEIN; TRANSMISSION ELECTRON MICROSCOPY;

LYSOZYME; NANOPARTICLE; PARATHYROID HORMONE RECEPTOR 1; PTH1R PROTEIN, HUMAN; RNA POLYMERASE II;

CHEMISTRY; CONFORMATION; ELECTRON; FOURIER ANALYSIS; HUMAN; LASER; PROCEDURES; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE; X RAY DIFFRACTION;

ELECTRONS; FOURIER ANALYSIS; HUMANS; LASERS; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR CONFORMATION; MURAMIDASE; NANOPARTICLES; RECEPTOR, PARATHYROID HORMONE, TYPE 1; RNA POLYMERASE II; X-RAY DIFFRACTION;

EID: 84902670110     PISSN: 09628436     EISSN: 14712970     Source Type: Journal    
DOI: 10.1098/rstb.2013.0322     Document Type: Article

References (21)

1. Neutze R, Wouts R, van der Spoel D, Weckert E, Hajdu J. 2000 Potential for biomolecular imaging with femtosecond X-ray pulses. Nature 406, 752-757. (doi: 10. 1038/35021099).
2. Chapman HN et al. 2011 Femtosecond X-ray protein nanocrystallography. Nature 470, 73-77. (doi: 10. 1038/nature09750).
3. Boutet S, Williams GJ. 2010 The Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS). New J. Phys. 12, 035024. (doi: 10. 1088/1367-2630/12/3/035024).
4. DePonte DP, Weierstall U, Schmidt K, Warner J, Starodub D, Spence JCH, Doak RB. 2008 Gas dynamic virtual nozzle for generation of microscopic droplet streams. J. Phys. D: Appl. Phys. 41, 195505. (doi: 10. 1088/0022-3727/41/19/195505).
5. Aquila A et al. 2012 Time-resolved protein nanocrystallography using an X-ray free-electron laser. Opt. Express 20, 2706-2716. (doi: 10. 1364/OE. 20. 002706).
6. Boutet S et al. 2012 High-resolution protein structure determination by serial femtosecond crystallography. Science 337, 362-364. (doi: 10. 1126/science. 1217737).
7. Hunter MS, Fromme P. 2011 Toward structure determination using membrane-protein nanocrystals and microcrystals. Methods 55, 387-404. (doi: 10. 1016/j. ymeth. 2011. 12. 006).
8. Kern J et al. 2013 Simultaneous femtosecond X-ray spectroscopy and diffraction of photosystem II at room temperature. Science 340, 491-495. (doi: 10. 1126/science. 1234273).
9. Koopmann R et al. 2012 In vivo protein crystallization opens new routes in structural biology. Nat. Methods 9, 259-262. (doi: 10. 1038/nmeth. 1859).
10. Redecke L et al. 2013 Natively inhibited Trypanosoma brucei cathepsin B structure determined by using an X-ray laser. Science 339, 227-230. (doi: 10. 1126/science. 1229663).
11. Johansson LC et al. 2012 Lipidic phase membrane protein serial femtosecond crystallography. Nat. Methods 9, 263-265. (doi: 10. 1038/nmeth. 1867).
12. Lomb L et al. 2011 Radiation damage in protein serial femtosecond crystallography using an X-ray free-electron laser. Phys. Rev. B 84, 214111. (doi: 10. 1103/PhysRevB. 84. 214111).
13. Barty A et al. 2012 Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements. Nat. Photonics 6, 35-40. (doi: 10. 1038/nphoton. 2011. 297).
14. Thomas RM. 2013 De novo protein crystal structure determination from X-ray free-electron laser data. Nature 505, 244-247. (doi: 10. 1038/nature12773).
15. DePonte DP, Doak RB, Hunter M, Liu Z, Weierstall U, Spence JCH. 2009 SEM imaging of liquid jets. Micron 40, 507-509. (doi: 10. 1016/j. micron. 2008. 12. 009).
16. Sierra RG et al. 2012 Nanoflow electrospinning serial femtosecond crystallography. Acta Cryst. D68, 1584-1587. (doi: 10. 1107/S0907444912038152).
17. Stevenson HP et al. In press. Use of Transmission Electron Microscopy to identify nanocrystals of challenging protein targets. Proc. Natl Acad. Sci. USA.
18. Pullara F et al. 2013 A general path for large-scale solubilization of cellular proteins: from membrane receptors to multiprotein complexes. Protein Expr. Purif. 87, 111-119. (doi: 10. 1016/j. pep. 2012. 10. 007).
19. Dieryck W, Noubhani AM, Coulon D, Santarelli X. 2003 Cloning, expression and two-step purification of recombinant His-tag enhanced green fluorescent protein over-expressed in Escherichia coli. J. Chromatogr. B 786, 153-159. (doi: 10. 1016/S1570-0232(02)00764-X).
20. Hart P et al. 2012 The CSPAD megapixel X-ray camera at LCLS. X-Ray free-electron lasers: beam diagnostics, beamline instrumentation, and applications. 8504, 85048C.
21. Sauter NK, Hattne J, Grosse-Kunstleve RW, Echols N. 2013 New python-based methods for data processing. Acta Crystallogr. D69, 1274-1282. (doi: 10. 1107/S0907444913000863).