Expression, characterization, and improvement of a newly cloned halohydrin dehalogenase from Agrobacterium tumefaciens and its application in production of epichlorohydrin

Journal of Industrial Microbiology and Biotechnology

Volumn 41, Issue 7, 2014, Pages 1145-1158

  Liu, Zhi Qiang ^a   Gao, Ai Cun ^a   Wang, Ya Jun ^a   Zheng, Yu Guo ^a   Shen, Yin Chu ^a  

^a ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

1,3 Dichloro 2 propanol; Epichlorohydrin; Halohydrin dehalogenase; Site directed mutagenesis

Indexed keywords

1,3 DICHLORO 2 PROPANOL; BACTERIAL ENZYME; EPICHLOROHYDRIN; HALOHYDRIN DEHALOGENASE; MUTANT PROTEIN; PROPANOL; UNCLASSIFIED DRUG; 1,3-DICHLORO-2-PROPANOL; ALPHA CHLOROHYDRIN; HYDROLASE; METAL;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; BIOCATALYST; BIOTRANSFORMATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SUBSTRATE COMPLEX; MOLECULAR CLONING; MOLECULAR DOCKING; NONHUMAN; PROTEIN EXPRESSION; REPRODUCIBILITY; SITE DIRECTED MUTAGENESIS; SYNTHESIS; THERMOSTABILITY; WILD TYPE; ANALOGS AND DERIVATIVES; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HALF LIFE TIME; MASS FRAGMENTOGRAPHY; METABOLISM; PH; TEMPERATURE;

AGROBACTERIUM TUMEFACIENS; ALPHA-CHLOROHYDRIN; BIOCATALYSIS; BIOTRANSFORMATION; CATALYTIC DOMAIN; CLONING, MOLECULAR; ENZYME STABILITY; EPICHLOROHYDRIN; ESCHERICHIA COLI; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HALF-LIFE; HYDROGEN-ION CONCENTRATION; HYDROLASES; METALS; MODELS, MOLECULAR; MOLECULAR DOCKING SIMULATION; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84902602398     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-014-1443-2     Document Type: Article

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