Reverse genetic morpholino approach using cardiac ventricular injection to transfect multiple difficult-to-target tissues in the zebrafish larva

Journal of Visualized Experiments

Volumn , Issue 88, 2014, Pages

  Konantz, Judith ^a   Antos, Christopher L ^a  

^a CENTER FOR REGENERATIVE THERAPIES DRESDEN   (Germany)

Author keywords

Caudal fin; Developmental Biology; Heart; Intraventricular injection; Issue 88; Knockdown; Larva; Morpholino; Regeneration; Zebrafish

Indexed keywords

EID: 84902590498     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51595     Document Type: Article

References (22)

1. Gemberlin, M., Bailey, T. J., Hyde, D. R., Poss, K. D. The zebrafish as a model for complex tissue regeneration. Trends in Genetics. 29, 611-620 (2013).
2. Suster, M. L., Abe, G., Schouw, A., & Kawakami, K. Transposon-mediated BAC transgenesis in zebrafish. Nature Protocols. 6, 2011 (1998).
3. Skromne, I., & Prince, V. E. Current perspectives in zebrafish reverse genetics: moving forward. Developmental Dynamics. 237, 861-882 (2008).
4. Zu, Y. et al. TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nature Protocols. 10, 329-332 (2013).
5. Bedell, V. M. et al. In vivo genome editing using a high-efficiency TALEN system. Nature. 491, 114-118 (2012).
6. Kizil, C., IItzsche, A., Kaslin, J., & Brand, M. Micromanipulation of gene expression in the adult zebrafish brain using cerebroventricular microinjection of morpholino oligonucleotides. J Vis Exp. (75), e50415 (2013).
7. Hyde, D., Godwin, A., & Thummel, R. In vivo electroporation of morpholinos into the regenerating adult zebrafish tail fin. J Vis Exp. (61), pii 3632 doi 3610.3791/3632 (2012).
8. Thummel, R., Bailey, T., & Hyde, D. In vivo electroporation of morpholinos into the adult zebrafish retina. J Vis Exp. (58), e3603 doi: 3610.3791/3603 (2011).
9. Rieger, S., Kulkarni, R. P., Darcy, D., Fraser, S. E., & Köster, R. W. Quantum dots are powerful multipurose vital labeling agents in zebrafish embryos. Developmental Dynamics. 234, 670-681 (2005).
10. Madhavan, M. et al. The role of Pax-6 in lens regeneration. Proceedings of the National Academy of Science U S A. 103, 14848-14853 (2006).
11. Gilmour, D. T., Jessen, J. R., & Lin, S. in Zebrafish: a practical approach. Vol. 261 eds Christiane Nüsslein-Volhar., & Ralf Dahm Ch. 5, 121-143 Oxford University Press, (2000).
12. Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nature Methods 9, 676-682 (2012).
13. Eisen, J. S., & Smith, J. C. Controlling morpholino experiments: don't stop making antisense. Development. 135, 1735-1743 (2008).
14. Bill, B. R., Petzold, A. M., Clark, K. J., Schimmenti, L. A., & Ekker, S. C. A primer for morpholino use in zebrafish. Zebrafish. 6, 69-77 (2009).
15. Hyatt, T. M., & Ekker, S. C. Vectors and techniques for ectopic gene expression in zebrafish. Methods Cell Biol. 59, 117-126 (1999).
16. Hans, S., Kaslin, J., Freudenreich, D., & Brand, M. Temporally controlled site-specific recombination in zebrafish. PLoS One. 4, e4640 (2009).
17. Lee, Y., Grill, S., Sanchez, A., Murphy-Ryan, M., & Poss, K. D. Fgf signaling instructs position-dependent growth rate during zebrafish fin regeneration. Development. 132, 5173-5183 (2005).
18. Liu, S. Zebrafish models for cancer. Annu. Re. Pathol. Mech. Dis. 6, 71-93 (2011).
19. Stoick-Cooper, C. L. et al. Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Development. 134, 479-489 (2007).
20. Shen, S. P., Aleksic, J., & Russell, S. Identifying targets of the Sox domain proteins Dichaete in the Drosophila CNS via targeted expression of dominant negative proteins. BMC Dev. Biol. 13, 1 (2013).
21. McNeish, J. D., Scott, W. J., & Jr. Potter, S. S. Legless, a novel mutation found in PHT1-1 transgenic mice. Science. 241, 837-839 (1988).
22. Covarrubias, L., Nishida, Y., Terao, M., D'Eustachio, P., & Mintz, B. Cellular DNA rearrangements and early developmental arrest caused by DNA insertion in transgenic mouse embryos. Mol. Cell. Biol. 7, 2243-2247 (1987)