Site-related differences in gene expression and bacterial densities in the mussel Bathymodiolus azoricus from the Menez Gwen and Lucky Strike deep-sea hydrothermal vent sites

Fish and Shellfish Immunology

Volumn 39, Issue 2, 2014, Pages 343-353

  Bettencourt, Raul ^a   Rodrigues, Mónica ^a   Barros, Inês ^a   Cerqueira, Teresa ^a   Freitas, Cátia ^a   Costa, Valentina ^a   Pinheiro, Miguel ^b   Egas, Conceição ^b   Santos, Ricardo Serrão ^a  

^a UNIVERSITY OF THE AZORES   (Portugal)

^b Biocant Biotechnology Innovation Center   (Portugal)

Author keywords

Bathymodiolus azoricus; Endosymbiont; FISH; Gene expression; Immune genes

Indexed keywords

PRIMER DNA; RNA 16S; TRANSCRIPTOME;

ANIMAL; ATLANTIC OCEAN; BIOLOGY; BIVALVE; DNA SEQUENCE; ECOSYSTEM; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION REGULATION; GENETICS; GEOGRAPHY; GILL; HYDROTHERMAL VENT; METABOLISM; MICROBIOLOGY; MICROFLORA; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPECIES DIFFERENCE; SYMBIOSIS;

ANIMALS; ATLANTIC OCEAN; BASE SEQUENCE; BIVALVIA; COMPUTATIONAL BIOLOGY; DNA PRIMERS; ECOSYSTEM; GENE EXPRESSION REGULATION; GEOGRAPHY; GILLS; HYDROTHERMAL VENTS; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROBIOTA; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY; SYMBIOSIS; TRANSCRIPTOME;

EID: 84902456978     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2014.05.024     Document Type: Article

References (40)

1. Armstrong P.B., Quigley J.P. Immune function of alpha 2-macroglobulin in invertebrates. Prog Mol Subcell Biol 1996, 15:101-130.
2. Bettencourt R., Pinheiro M., Egas C., Gomes P., Afonso M., Shank T., et al. Sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus using 454 GSFLX. BMC Genomics 2010, 11:559.
3. Bettencourt R., Costa V., Laranjo M., Rosa D., Pires L., Colaço A., et al. Out of the deep-sea into a land-based aquarium environment: investigating innate immunity in the hydrothermal vent mussel Bathymodiolus azoricus. Cah Biol Mar 2010, 51:341-350.
4. Bettencourt R., Dando P., Collins P., Costa V., Allam B., Serrão Santos R. Innate immunity in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus. Comp Biochem Physiol A 2009, 152:278-289.
5. Bettencourt R., Dando P., Rosa D., Riou V., Colaço A., Sarrazin J., et al. Changes of gill and hemocyte- related bio-indicators during long term maintenance of the vent mussel Bathymodiolus azoricus held in aquaria at atmospheric pressure. Comp Biochem Physiol A 2008, 150:1-7.
6. Boutet I., Ripp R., Lecompte O., Dossat C., Corre E., Tanguy A., et al. Conjugating effects of symbionts and environmental factors on gene expression in deep-sea hydrothermal vent mussels. BMC Genomics 2011, 12:530. 10.1186/1471-2164-12-530.
7. Charlou J.L., Donval J.P., Douville E., Jean-Baptiste P., Radford-Knoery J., Fouquet Y., et al. Compared geochemical signatures and the evolution of Menez Gwen (37°50'N) and Lucky Strike (37°17'N) hydrothermal fluids, south of the Azores Triple junction on the Mid-Atlantic Ridge. Chem Geol 2000, 171:49-75.
8. Chen G., Goeddel D.V. TNF-R1 signaling: a beautiful pathway. Science 2002, 296(5573):1634-1635.
9. Childress J.J., Fisher C.R. The biology of hydrothermal vent animals-physiology, biochemistry, and autotrophic symbioses. Oceanogr Mar Biol Ann Rev 1992, 30:337-441.
10. 13C signature of hydrothermal mussels is related with the end-member fluid concentrations of H2S and CH4 at the Mid-Atlantic Ridge hydrothermal vent sites. Cah Biol Mar 2002, 43:259-262.
11. Desbruyères D., Almeida A., Biscoito M., Comtet T., Khripounoff A., Le Bris N., et al. Areview of the distribution of hydrothermal vent communities along the northern Mid-Atlantic Ridge: dispersal vs. environmental controls. Hydrobiologia 2000, 400:201-216.
12. Distel D.L., Lee H.K.W., Cavanaugh C.M. Intracellular coexistence of methanotrophic and Thioautotrophic bacteria in a hydrothermal vent mussel. Proc Natl Acad Sci U S A 1995, 92:9598-9602.
13. Duperron S., Nadalig T., Caprais J.-C., Sibuet M., Fiala-Médioni A., Rudolf A., et al. Dual symbiosis in a Bathymodiolus sp. Mussel from a methane seep on the Gabon Continental Margin (Southeast Atlantic): 16S rRNA phylogeny and distribution of the symbionts in gills. Appl Environ Microbiol 2005, 71(4):1694-1700.
14. Duperron S., Bergin C., Zielinski F., Blazejak A., Pernthaler A., McKiness Z.P., et al. Adual symbiosis shared by two mussel species, Bathymodiolus azoricus and Bathymodiolus puteoserpentis (Bivalvia: Mytilidae), from hydrothermal vents along the northern Mid-Atlantic Ridge. Environ Microbiol 2006, 8:1441-1447.
15. Duperron S., Lorion J., Samadi S., Gros O., Gaill F. Symbioses between deep-sea mussels (Mytilidae: Bathymodiolinae) and chemosynthetic bacteria: diversity, function and evolution. C.R. Biol 2009, 332:298-310.
16. Duperron S., Gaudron M., Rodrigues C.F., Cunha M.R., Decker C., Olu K. An overview of chemosynthetic symbioses in bivalves from the North Atlantic and Mediterranean Sea. Biogeosciences 2013, 10:3241-3267. 10.5194/bg-10-3241-2013.
17. Dziarski R., Gupta D. The peptidoglycan recognition proteins (PGRPs). Genome Biol 2006, 7:232,. 10.1186/gb-2006-7-8-232.
18. Edgar R.C. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 2010, 26:2460-2461.
19. Egas C., Pinheiro M., Gomes P., Barroso C., Bettencourt R. Transcriptome of Bathymodiolus azoricus gill reveals expression of genes from endosymbionts and free-living deep-sea bacteria. Mar Drugs 2012, 10(8):1765-1783.
20. Fiala-Médioni A., McKiness Z.P., Dando P., Boulegue J., Mariotti A., Alayse-Danet A.M., et al. Ultrastructural, biochemical, and immunological characterization of two populations of the mytilid mussel Bathymodiolus azoricus from the Mid-Atlantic Ridge: evidence for a dual symbiosis. Mar Biol 2002, 141:1035-1043.
21. Fisher C.R., Brooks J.M., Childress J.J., Felbeck H., Hessler R.R., Johnson K.S., et al. Microhabitat requirements of the hydrothermal vent mussel, Bathymodiolus thermophilus. Am Zool 1989, 29. A81-A81.
22. Gustafson R.G., Turner R.D., Lutz R.A., Vrijenhoek R.C. Anew genus and five new species os mussels (Bivalvia, Mytilidae) from deep-sea sulphide/hydrocarbon seeps in the Gulf of Mexico. Malacologia 1998, 40:63-112.
23. Halary S., Riou V., Gaill F., Boudier T., Duperron S. 3D FISH for the quantification of methane- and sulphur-oxidizing endosymbionts in bacteriocytes of the hydrothermal vent mussel Bathymodiolus azoricus. ISME J 2008, 2:284-292.
24. Huang X., Madan A. CAP3: a DNA sequence assembly program. Genome Res 1999, 9:868-877.
25. Kadar E., Costa V. Post-capture experimental investigations using hydrothermal vent macro-invertebrates to study adaptations to extreme environments. Rev Environ Sci Bio/Technol 2006, 5:193-201.
26. Lane D.J. 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics 1991, 115-175. John Wiley and Sons, New York, NY. E. Stackebrandt, M. Goodfellow (Eds.).
27. Lee R.W., Thuesen E.V., Childress J.J., Fisher C.R. Ammonium and free amino acid uptake by a deep-sea mussel containing methanotrophic bacterial symbionts. Mar Biol 1992, 113:99-106.
28. Nagase H., Woessner J.F. Matrix metalloproteinases. JBiol Chem 1999 Jul 30, 274(31):21491-21494.
29. Nyholm S., Song P., Dang J., Bunce C., Girguis P.R. Expression and putative function of innate immunity genes under in situ conditions in the symbiotic hydrothermal vent tubeworm Ridgeia piscesae. PLoS One 2012, 7(6):e38267. 10.1371/journal.pone.0038267.
30. Page H.M., Fiala-Médioni A., Childress J.J. Experimental evidence for filter-feeding by the hydrothermal vent mussel, Bathymodiolus thermophilus. Deep Sea Res Part A 1991, 38(12):1455-1461.
31. Parks W.C., Wilson C.L., López-Boado Y.S. Matrix metalloproteinases as modulators of inflammation and innate immunity. Nat Rev Immunol 2004, 4:617-629.
32. Ravaux J., Hamel G., Zbinden, Tasiemski A.A., Boutet I., Léger N., et al. Thermal limit for metazoan life in question: invivo heat tolerance of the pompeii worm. PLoS One 2013, 8(5):e64074. 10.1371/journal.pone.0064074.
33. Riou V., Halary S., Duperron S., Bouillon S., Elskens M., Bettencourt R., et al. Influence of chemosynthetic substrates availability on symbiont densities, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus. Biogeoscience 2008, 5(6):1681-1691. 10.5194/bgd-5-2279-2008.
34. Swaminathan C.P., Brown P.H., Roychowdhury A., Wang Q., Guan R., Silverman N., et al. Dual strategies for peptidoglycan discrimination by peptidoglycan recognition proteins (PGRPs). PNAS 2006, 103(3):684-689. [MMUNOLOGY].
35. Tang X., Metzger D., Leeman S., Amar S. LPS-induced TNF- factor (LITAF)-deficient mice express reduced LPS-induced cytokine: evidence for LITAF-dependent LPS signaling pathways. PNAS 2006, 103(37):13777-13782.
36. Traver M.K., Henry S.C., Cantillana V., Oliver T., Hunn J.P., Howard J.C., et al. Immunity-related GTPase m (IRGM) proteins influence the localization of guanylate-binding protein 2 (GBP2) by modulating macroautophagy. JBiol Chem 2011, 286(35):30471-30480.
37. Turner S., Pryer K.M., Miao V.P.W., Palmer J.D. Investigating deep phylogenetic relationships among cyanobacteria and plastids by small subunit rRNA sequence analysis. JEukaryot Microbiol 1999, 46:327-338.
38. Von Cosel R., Comtet T., Krylova E.M. Bathymodiolus (Bivalvia: Mytilidae) from hydrothermal vents on the Azores triple junction and the Logatchev hydrothermal field, Mid Atlantic Ridge. Veliger 1999, 42:218-248.
39. Wang Y., Qian P.-Y. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. PLoS One 2009, 4(10):e7401. 10.1371/journal.pone.0007401.
40. Wendeberg A., Zielinski F.U., Borowski C., Dubilier N. Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the hydrothermal vent mussel Bathymodiolus puteoserpentis. ISME J 2012, 6:104-112. 10.1038/ismej.2011.81.