Human cytomegalovirus replication is strictly inhibited by siRNAs targeting UL54, UL97 or UL122/123 gene transcripts

PLoS ONE

Volumn 9, Issue 6, 2014, Pages

  Hamilton, Stuart T ^a,b   Milbradt, Jens ^c   Marschall, Manfred ^c   Rawlinson, William D ^a,b  

^a PRINCE OF WALES HOSPITAL   (Australia)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SMALL INTERFERING RNA; DNA DIRECTED DNA POLYMERASE; GANCICLOVIR KINASE; IE1 PROTEIN, CYTOMEGALOVIRUS; IE2 PROTEIN, CYTOMEGALOVIRUS; IMMEDIATE EARLY PROTEIN; MESSENGER RNA; PHOSPHOTRANSFERASE; TRANSACTIVATOR PROTEIN; UL54 PROTEIN, HUMAN HERPESVIRUS 5; VIRUS PROTEIN;

ARTICLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; EMBRYO; FIBROBLAST; GENE TARGETING; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PROGENY; PROTEIN EXPRESSION; UL122 GENE; UL123 GENE; UL54 GENE; UL97 GENE; VIRUS CULTURE; VIRUS GENE; VIRUS LOAD; VIRUS REPLICATION; VIRUS STRAIN; WESTERN BLOTTING; CELL LINE; GENE EXPRESSION REGULATION; GENETICS; INTRACELLULAR SPACE; METABOLISM; PHYSIOLOGY; PLASMID;

CELL LINE; CYTOMEGALOVIRUS; DNA-DIRECTED DNA POLYMERASE; GENE EXPRESSION REGULATION, VIRAL; GENES, VIRAL; HUMANS; IMMEDIATE-EARLY PROTEINS; INTRACELLULAR SPACE; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANS-ACTIVATORS; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 84902308423     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0097231     Document Type: Article

References (38)

1. Hamilton ST, Scott G, Naing Z, Iwasenko J, Hall B, et al. (2012) Human cytomegalovirus-induces cytokine changes in the placenta with implications for adverse pregnancy outcomes. PLoS One 7: e52899.
2. Demmler GJ (1991) Infectious Diseases Society of America and Centers for Disease Control. Summary of a workshop on surveillance for congenital cytomegalovirus disease. Rev Infect Dis 13: 315-329.
3. Wang C, Zhang X, Bialek S, Cannon MJ (2011) Attribution of congenital cytomegalovirus infection to primary versus non-primary maternal infection. Clin Infect Dis 52: e11-13.
4. Lischka P, Hewlett G, Wunberg T, Baumeister J, Paulsen D, et al. (2010) In vitro and in vivo activities of the novel anticytomegalovirus compound AIC246. Antimicrob Agents Chemother 54: 1290-1297.
5. Marschall M, Stamminger T, Urban A, Wildum S, Ruebsamen-Schaeff H, et al. (2012) In vitro evaluation of the activities of the novel anticytomegalovirus compound AIC246 (letermovir) against herpesviruses and other human pathogenic viruses. Antimicrob Agents Chemother 56: 1135-1137.
6. Goldner T, Hewlett G, Ettischer N, Ruebsamen-Schaeff H, Zimmermann H, et al. (2011) The novel anticytomegalovirus compound AIC246 (Letermovir) inhibits human cytomegalovirus replication through a specific antiviral mechanism that involves the viral terminase. J Virol 85: 10884-10893.
7. Devi GR (2006) siRNA-based approaches in cancer therapy. Cancer Gene Ther 13: 819-829.
8. Xiaofei E, Stadler BM, Debatis M, Wang S, Lu S, et al. (2012) RNA interference-mediated targeting of human cytomegalovirus immediate-early or early gene products inhibits viral replication with differential effects on cellular functions. J Virol 86: 5660-5673.
9. Shin MC, Hong SK, Yoon JS, Park SS, Lee SG, et al. (2006) Inhibition of UL54 and UL97 genes of human cytomegalovirus by RNA interference. Acta Virol 50: 263-268.
10. Wiebusch L, Truss M, Hagemeier C (2004) Inhibition of human cytomegalovirus replication by small interfering RNAs. J Gen Virol 85: 179-184. (Pubitemid 38128883)
11. Wang K, Li Y, Zhao G, Wu Y, Zhang X, et al. (2013) Inhibition of human cytomegalovirus DNA replication by small interfering RNAs targeted to UL49. Acta Biochim Biophys Sin (Shanghai) 45: 401-407.
12. Duan QJ, Tao R, Hu MF, Shang SQ (2009) Efficient inhibition of human cytomegalovirus UL122 gene expression in cell by small interfering RNAs. J Basic Microbiol 49: 531-537.
13. Tao R, Hu M, Duan Q, Shang S (2008) Efficient inhibition of human cytomegalovirus DNA polymerase expression by small hairpin RNA in vitro. Curr Microbiol 57: 395-400.
14. Bai Z, Li L, Wang B, Liu Z, Liu H, et al. (2010) Inhibition of human cytomegalovirus infection by IE86-specific short hairpin RNA-mediated RNA interference. Biosci Biotechnol Biochem 74: 1368-1372.
15. Nekhai S, Jerebtsova M (2006) Therapies for HIV with RNAi. Curr Opin Mol Ther 8: 52-61. (Pubitemid 43230901)
16. Wu J, Nandamuri KM (2004) Inhibition of hepatitis viral replication by siRNA. Expert Opin Biol Ther 4: 1649-1659. (Pubitemid 39371760)
17. Kapadia SB, Brideau-Andersen A, Chisari FV (2003) Interference of hepatitis C virus RNA replication by short interfering RNAs. Proc Natl Acad Sci U S A 100: 2014-2018. (Pubitemid 36254565)
18. Gitlin L, Karelsky S, Andino R (2002) Short interfering RNA confers intracellular antiviral immunity in human cells. Nature 418: 430-434. (Pubitemid 34826844)
19. Butz K, Ristriani T, Hengstermann A, Denk C, Scheffner M, et al. (2003) siRNA targeting of the viral E6 oncogene efficiently kills human papillomaviruspositive cancer cells. Oncogene 22: 5938-5945. (Pubitemid 37175872)
20. Ge Q, McManus MT, Nguyen T, Shen CH, Sharp PA, et al. (2003) RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc Natl Acad Sci U S A 100: 2718-2723. (Pubitemid 36297565)
21. Sadanari H, Yamada R, Ohnishi K, Matsubara K, Tanaka J (2005) SUMO-1 modification of the major immediate-early (IE) 1 and 2 proteins of human cytomegalovirus is regulated by different mechanisms and modulates the intracellular localization of the IE1, but not IE2, protein. Arch Virol 150: 1763-1782. (Pubitemid 41176634)
22. Marchini A, Liu H, Zhu H (2001) Human cytomegalovirus with IE-2 (UL122) deleted fails to express early lytic genes. J Virol 75: 1870-1878. (Pubitemid 32110177)
23. Isomura H, Tsurumi T, Stinski MF (2004) Role of the proximal enhancer of the major immediate-early promoter in human cytomegalovirus replication. J Virol 78: 12788-12799. (Pubitemid 39507787)
24. Sinclair J, Sissons P (2006) Latency and reactivation of human cytomegalovirus. J Gen Virol 87: 1763-1779. (Pubitemid 43985862)
25. Ahn JH, Jang WJ, Hayward GS (1999) The human cytomegalovirus IE2 and UL112-113 proteins accumulate in viral DNA replication compartments that initiate from the periphery of promyelocytic leukemia protein-associated nuclear bodies (PODs or ND10). J Virol 73: 10458-10471.
26. Kerry JA, Priddy MA, Jervey TY, Kohler CP, Staley TL, et al. (1996) Multiple regulatory events influence human cytomegalovirus DNA polymerase (UL54) expression during viral infection. J Virol 70: 373-382.
27. Isomura H, Stinski MF, Kudoh A, Murata T, Nakayama S, et al. (2008) Noncanonical TATA sequence in the UL44 late promoter of human cytomegalovirus is required for the accumulation of late viral transcripts. J Virol 82: 1638-1646. (Pubitemid 351206915)
28. Hertel L, Chou S, Mocarski ES (2007) Viral and cell cycle-regulated kinases in cytomegalovirus-induced pseudomitosis and replication. PLoS Pathog 3: e6.
29. Graf L, Webel R, Wagner S, Hamilton ST, Rawlinson WD, et al. (2013) The cyclin-dependent kinase ortholog pUL97 of human cytomegalovirus interacts with cyclins. Viruses 5: 3213-3230.
30. Chou S, Van Wechel LC, Marousek GI (2006) Effect of cell culture conditions on the anticytomegalovirus activity of maribavir. Antimicrob Agents Chemother 50: 2557-2559. (Pubitemid 43993205)
31. Bigley TM, Reitsma JM, Mirza SP, Terhune SS (2013) Human cytomegalovirus pUL97 regulates the viral major immediate early promoter by phosphorylationmediated disruption of histone deacetylase 1 binding. J Virol 87: 7393-7408.
32. Marschall M, Feichtinger S, Milbradt J (2011) Regulatory roles of protein kinases in cytomegalovirus replication. Adv Virus Res 80: 69-101.
33. Zheng ZM, Tang S, Tao M (2005) Development of resistance to RNAi in mammalian cells. Ann N Y Acad Sci 1058: 105-118.
34. Sanjuan R, Nebot MR, Chirico N, Mansky LM, Belshaw R (2010) Viral mutation rates. J Virol 84: 9733-9748.
35. Das AT, Brummelkamp TR, Westerhout EM, Vink M, Madiredjo M, et al. (2004) Human immunodeficiency virus type 1 escapes from RNA interferencemediated inhibition. J Virol 78: 2601-2605. (Pubitemid 38228957)
36. Renzette N, Bhattacharjee B, Jensen JD, Gibson L, Kowalik TF (2011) Extensive genome-wide variability of human cytomegalovirus in congenitally infected infants. PLoS Pathog 7: e1001344.
37. Renzette N, Gibson L, Bhattacharjee B, Fisher D, Schleiss MR, et al. (2013) Rapid intrahost evolution of human cytomegalovirus is shaped by demography and positive selection. PLoS Genet 9: e1003735.
38. Hamilton ST, Scott GM, Naing Z, Rawlinson WD (2013) Human cytomegalovirus directly modulates expression of chemokine CCL2 (MCP-1) during viral replication. J Gen Virol 94: 2495-2503.