Time-resolved fluorescence spectroscopy measures clustering and mobility of a G protein-coupled receptor opsin in live cell membranes

Journal of the American Chemical Society

Volumn 136, Issue 23, 2014, Pages 8342-8349

  Comar, William D ^a   Schubert, Sarah M ^a,c   Jastrzebska, Beata ^b   Palczewski, Krzysztof ^b   Smith, Adam W ^a  

^a UNIVERSITY OF AKRON   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GROVE CITY COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; DIMERS; EQUILIBRIUM CONSTANTS; FLUORESCENCE SPECTROSCOPY; MONOMERS;

FLUORESCENCE CROSS-CORRELATION SPECTROSCOPY; G PROTEIN COUPLED RECEPTORS; INDIVIDUAL CELLS; LOW CONCENTRATIONS; MEMBRANE PROTEINS; MONOMER-DIMER EQUILIBRIUM; TIME-RESOLVED FLUORESCENCE SPECTROSCOPY;

CELL MEMBRANES;

DIMER; G PROTEIN COUPLED RECEPTOR; MEMBRANE PROTEIN; MONOMER; OLIGOMER; OPSIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; EQUILIBRIUM CONSTANT; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE SPECTROSCOPY; MULTIPHOTON MICROSCOPY; NONHUMAN; OLIGOMERIZATION; PROTEIN QUATERNARY STRUCTURE; ANIMAL; CHEMISTRY; CHLOROCEBUS AETHIOPS; COS 1 CELL LINE; CULTURE TECHNIQUE; GENETIC TRANSFECTION; GENETICS; METABOLISM; PROCEDURES; PROTEIN MULTIMERIZATION; PROTEIN TRANSPORT; SPECTROFLUOROMETRY;

ANIMALS; CELL CULTURE TECHNIQUES; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; COS CELLS; OPSINS; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN TRANSPORT; RECEPTORS, G-PROTEIN-COUPLED; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

EID: 84902265013     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja501948w     Document Type: Article

References (34)

1. Khelashvili, G.; Dorff, K.; Shan, J.; Camacho-Artacho, M.; Skrabanek, L.; Vroling, B.; Bouvier, M.; Devi, L. A.; George, S. R.; Javitch, J. A.; Lohse, M. J.; Milligan, G.; Neubig, R. R.; Palczewski, K.; Parmentier, M.; Pin, J.-P.; Vriend, G.; Campagne, F.; Filizola, M. Bioinformatics 2010, 26, 1804-1805
2. Lambert, N. A. Sci. Signaling 2010, 3, pe12
3. Milligan, G. Mol. Pharmacol. 2013, 84, 158-169
4. Bortolato, A.; Mobarec, J. C.; Provasi, D.; Filizola, M. Curr. Pharm. Des. 2009, 15, 4017-4025
5. Palczewski, K.; Kumasaka, T.; Hori, T.; Behnke, C. A.; Motoshima, H.; Fox, B. A.; Trong, I. L.; Teller, D. C.; Okada, T.; Stenkamp, R. E.; Yamamoto, M.; Miyano, M. Science 2000, 289, 739-745
6. Palczewski, K. J. Biol. Chem. 2012, 287, 1612-1619
7. Nickell, S.; Park, P. S.-H.; Baumeister, W.; Palczewski, K. J. Cell Biol. 2007, 177, 917-925
8. Poo, M.-m.; Cone, R. A. Nature 1974, 247, 438-441
9. Liebman, P. A.; Entine, G. Science 1974, 185, 457-459
10. Ernst, O. P.; Gramse, V.; Kolbe, M.; Hofmann, K. P.; Heck, M. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 10859-10864
11. Bayburt, T. H.; Vishnivetskiy, S. A.; McLean, M. A.; Morizumi, T.; Huang, C.-c.; Tesmer, J. J. G.; Ernst, O. P.; Sligar, S. G.; Gurevich, V. V. J. Biol. Chem. 2011, 286, 1420-1428
12. Fotiadis, D.; Liang, Y.; Filipek, S.; Saperstein, D. A.; Engel, A.; Palczewski, K. Nature 2003, 421, 127-128
13. Jastrzebska, B.; Ringler, P.; Palczewski, K.; Engel, A. J. Struct. Biol. 2013, 182, 164-172
14. Mansoor, S. E.; Palczewski, K.; Farrens, D. L. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 3060-3065
15. Müller, B. K.; Zaychikov, E.; Bräuchle, C.; Lamb, D. C. Biophys. J. 2005, 89, 3508-3522
16. Endres, N. F.; Das, R.; Smith, A. W.; Arkhipov, A.; Kovacs, E.; Huang, Y.; Pelton, J. G.; Shan, Y.; Shaw, D. E.; Wemmer, D. E.; Groves, J. T.; Kuriyan, J. Cell 2013, 152, 543-556
17. Triffo, S. B.; Huang, H. H.; Smith, A. W.; Chou, E. T.; Groves, J. T. J. Am. Chem. Soc. 2012, 134, 10833-10842
18. O'Shea, E. K.; Klemm, J. D.; Kim, P. S.; Alber, T. Science 1991, 254, 539-44
19. Rodgers, W. Biotechniques 2002, 32, 1044
20. Foo, Y. H.; Naredi-Rainer, N.; Lamb, D. C.; Ahmed, S.; Wohland, T. Biophys. J. 2012, 102, 1174-1183
21. Larson, D. R.; Gosse, J. A.; Holowka, D. A.; Baird, B. A.; Webb, W. W. J. Cell Biol. 2005, 171, 527-536
22. Sudhaharan, T.; Liu, P.; Foo, Y. H.; Bu, W.; Lim, K. B.; Wohland, T.; Ahmed, S. J. Biol. Chem. 2009, 284, 13602-13609
23. Gambin, Y.; Lopez-Esparza, R.; Reffay, M.; Sierecki, E.; Gov, N. S.; Genest, M.; Hodges, R. S.; Urbach, W. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 2098-2102
24. Saffman, P. G.; Delbruck, M. Proc. Natl. Acad. Sci. U.S.A. 1975, 72, 3111-3113
25. Naji, A.; Levine, A. J.; Pincus, P. A. Biophys. J. 2007, 93, L49-L51
26. Ly, S.; Bourguet, F.; Fischer, N. O.; Lau, E. Y.; Coleman, M. A.; Laurence, T. A. Biophys. J. 2014, 106, L05-L08
27. Guo, L.; Har, J. Y.; Sankaran, J.; Hong, Y.; Kannan, B.; Wohland, T. ChemPhysChem 2008, 9, 721-728
28. Herrick-Davis, K.; Grinde, E.; Cowan, A.; Mazurkiewicz, J. E. Mol. Pharmacol. 2013, 84, 630-642
29. Herrick-Davis, K.; Grinde, E.; Lindsley, T.; Cowan, A.; Mazurkiewicz, J. E. J. Biol. Chem. 2012, 287, 23604-23614
30. Kota, P.; Reeves, P. J.; RajBhandary, U. L.; Khorana, H. G. Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 3054-3059
31. Albertazzi, L.; Arosio, D.; Marchetti, L.; Ricci, F.; Beltram, F. Photochem. Photobiol. 2008, 9999
32. Jura, N.; Endres, N. F.; Engel, K.; Deindl, S.; Das, R.; Lamers, M. H.; Wemmer, D. E.; Zhang, X.; Kuriyan, J. Cell 2009, 137, 1293-1307
33. Kasai, R. S.; Suzuki, K. G. N.; Prossnitz, E. R.; Koyama-Honda, I.; Nakada, C.; Fujiwara, T. K.; Kusumi, A. J. Cell Biol. 2011, 192, 463-480
34. Bacia, K.; Kim, S. A.; Schwille, P. Nat. Methods 2006, 3, 83-89