Applications of silk biomaterials in tissue engineering and regenerative medicine

Silk Biomaterials for Tissue Engineering and Regenerative Medicine

Volumn , Issue , 2014, Pages 41-77

  Das, S ^a   Bora, U ^a   Borthakur, B B ^a,b  

^a INDIAN INSTITUTE OF TECHNOLOGY GUWAHATI   (India)

^b Dr Bhubaneswar Borooah Cancer Institute ^*  (India)

Author keywords

Biomaterial; Fibroin; Neotissue; Scaffold; Sericin; Silk; Tissue engineering

Indexed keywords

BIOMATERIALS; SCAFFOLDS; SILK; TISSUE; TISSUE ENGINEERING;

CLINICAL TRANSLATION; FIBROIN; NEOTISSUE; PHYSICO-CHEMICALS; REGENERATIVE MEDICINE; SERICIN; SURGICAL SUTURES; TISSUE ENGINEERED PRODUCTS;

SCAFFOLDS (BIOLOGY);

EID: 84902227499     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1533/9780857097064.1.41     Document Type: Chapter

References (186)

1. Kim H.J., Kim U.J., Vunjak-Novakovic G., Min B.H., Kaplan D.L. Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells. Biomaterials 2005, 26(21):4442-4452.
2. Meinel L., Fajardo R., Hofmann S., Langer R., Chen J., Snyder B., Vunjak-Novakovic G., Kaplan D. Silk implants for the healing of critical size bone defects. Bone 2005, 37(5):688-698.
3. Meinel L., Betz O., Fajardo R., Hofmann S., Nazarian A., Cory E., Hilbe M., McCool J., Langer R., Vunjak-Novakovic G., Merkle H.P., Rechenberg B., Kaplan D.L., Kirker-Head C. Silk based biomaterials to heal critical sized femur defects. Bone 2006, 39(4):922-931.
4. Meinel L., Hofmann S., Betz O., Fajardo R., Merkle H.P., Langer R., Evans C.H., Vunjak-Novakovic G., Kaplan D.L. Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials 2006, 27(28):4993-5002.
5. Li C., Vepari C., Jin H.J., Kim H.J., Kaplan D.L. Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 2006, 27(16):3115-3124.
6. Kirker-Head C., Karageorgiou V., Hofmann S., Fajardo R., Betz O., Merkle H.P., Hilbe M., von Rechenberg B., McCool J., Abrahamsen L., Nazarian A., Cory E., Curtis M., Kaplan D., Meinel L. BMP-silk composite matrices heal critically sized femoral defects. Bone 2007, 41(2):247-255.
7. Hofmann S., Hagenmüller H., Koch A.M., Müller R., Vunjak-Novakovic G., Kaplan D.L., Merkle H.P., Meinel L. Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials 2007, 28(6):1152-1162.
8. Kim H.J., Kim U.J., Leisk G.G., Bayan C., Georgakoudi I., Kaplan D.L. Bone regeneration on macroporous aqueous-derived silk 3-D scaffolds. Macromol Biosci 2007, 7(5):643-655.
9. Marolt D., Augst A., Freed L.E., Vepari C., Fajardo R., Patel N., Gray M., Farley M., Kaplan D., Vunjak-Novakovic G. Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors. Biomaterials 2006, 27(36):6138-6149.
10. Morgan A.W., Roskov K.E., Lin-Gibson S., Kaplan D.L., Becker M.L., Simon C.G. Characterization and optimization of RGD-containing silk blends to support osteoblastic differentiation. Biomaterials 2008, 29(16):2556-2563.
11. Mandal B.B., Kundu S.C. Non-mulberry silk gland fibroin protein 3-D scaffold for enhanced differentiation of human mesenchymal stem cells into osteocytes. Acta Biomater 2009, 5(7):2579-2590.
12. Jones G.L., Motta A., Marshall M.J., El Haj A.J., Cartmell S.H. Osteoblast: osteoclast co-cultures on silk fibroin, chitosan and PLLA films. Biomaterials 2009, 30(29):5376-5384.
13. Kim H.J., Kim U.J., Kim H.S., Li C., Wada M., Leisk G.G., Kaplan D.L. Bone tissue engineering with premineralized silk scaffolds. Bone 2008, 42(6):1226-1234.
14. Jiang X., Zhao J., Wang S., Sun X., Zhang X., Chen J., Kaplan D.L., Zhang Z. Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs. Biomaterials 2009, 30(27):4522-4532.
15. Wei K., Li Y., Kim K.O., Nakagawa Y., Kim B.S., Abe K., Chen G.Q., Kim I.S. Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior. J Biomed Mater Res A 2011, 97(3):272-280.
16. Fuchs S., Jiang X., Schmidt H., Dohle E., Ghanaati S., Orth C., Hofmann A., Motta A., Migliaresi C., Kirkpatrick C.J. Dynamic processes involved in the prevascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells. Biomaterials 2009, 30(7):1329-1338.
17. Correia C., Bhumiratana S., Yan L.P., Oliveira A.L., Gimble J.M., Rockwood D., Kaplan D.L., Sousa R.A., Reis R.L., Vunjak-Novakovic G. Development of silk-based scaffolds for tissue engineering of bone from human adipose-derived stem cells. Acta Biomater 2012, 8(7):2483-2492.
18. Marelli B., Ghezzi C.E., Alessandrino A., Barralet J.E., Freddi G., Nazhat S.N. Silk fibroin derived polypeptide-induced biomineralization of collagen. Biomaterials 2012, 33(1):102-108.
19. Almarza A.J., Athanasiou K.A. Design characteristics for the tissue engineering of cartilaginous tissues. Ann Biomed Eng 2004, 32(1):2-17.
20. Morita Y., Tomita N., Aoki H., Wakitani S., Tamada Y., Suguro T., Ikeuchi K. Visco-elastic properties of cartilage tissue regenerated with fibroin sponge. Biomed Mater Eng 2002, 12(3):291-298.
21. Aoki H., Tomita N., Morita Y., Hattori K., Harada Y., Sonobe M., Wakitani S., Tamada Y. Culture of chondrocytes in fibroin-hydrogel sponge. Biomed Mater Eng. 2003, 13(4):309-316.
22. Meinel L., Hofmann S., Karageorgiou V., Zichner L., Langer R., Kaplan D., Vunjak-Novakovic G. Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. Biotechnol Bioeng 2004, 88(3):379-391.
23. Hofmann S., Knecht S., Langer R., Kaplan D.L., Vunjak-Novakovic G., Merkle H.P., Meinel L. Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. Tissue Eng. 2006, 12(10):2729-2738.
24. Wang Y., Blasioli D.J., Kim H.J., Kim H.S., Kaplan D.L. Cartilage tissue engineering with silk scaffolds and human articular chondrocytes. Biomaterials 2006, 27(25):4434-4442.
25. Wang Y., Kim U.J., Blasioli D.J., Kim H.J., Kaplan D.L. In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells. Biomaterials 2005, 26(34):7082-7094.
26. Yamamoto K., Tomita N., Fukuda Y., Suzuki S., Igarashi N., Suguro T., Tamada Y. Time-dependent changes in adhesive force between chondrocytes and silk fibroin substrate. Biomaterials 2007, 28(10):1838-1846.
27. Morita Y., Tomita N., Aoki H., Sonobe M., Wakitani S., Tamada Y., Suguro T., Ikeuchi K. Frictional properties of regenerated cartilage in vitro. J Biomech 2006, 39(1):103-109.
28. Uebersax L., Merkle H.P., Meinel L. Insulin-like growth factor I releasing silk fibroin scaffolds induce chondrogenic differentiation of human mesenchymal stem cells. J Control Release 2008, 127(1):12-21.
29. Kambe Y., Yamamoto K., Kojima K., Tamada Y., Tomita N. Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis. Biomaterials 2010, 31(29):7503-7511.
30. Augst A., Marolt D., Freed L.E., Vepari C., Meinel L., Farley M., Fajardo R., Patel N., Gray M., Kaplan D.L., Vunjak-Novakovic G. Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors. J R Soc Interface 2008, 5(25):929-939.
31. Wang Y., Bella E., Lee C.S., Migliaresi C., Pelcastre L., Schwartz Z., Boyan B.D., Motta A. The synergistic effects of 3-D porous silk fibroin matrix scaffold properties and hydrodynamic environment in cartilage tissue regeneration. Biomaterials 2010, 31(17):4672-4681.
32. Tiǧli R.S., Cannizaro C., Gü̧derelioǧlu M., Kaplan D.L. Chondrogenesis in perfusion bioreactors using porous silk scaffolds and hESC-derived MSCs. J Biomed Mater Res A 2011, 96(1):21-28.
33. Chlapanidas T., Faragò S., Mingotto F., Crovato F., Tosca M.C., Antonioli B., Bucco M., Lucconi G., Scalise A., Vigo D., Faustini M., Marazzi M., Torre M.L. Regenerated silk fibroin scaffold and infrapatellar adipose stromal vascular fraction as feeder-layer: a new product for cartilage advanced therapy. Tissue Eng Part A 2011, 17(13-14):1725-1733.
34. Li F., Chen Y.Z., Miao Z.N., Zheng S.Y., Jin J. Human placenta-derived mesenchymal stem cells with silk fibroin biomaterial in the repair of articular cartilage defects. Cell Reprogram 2012, 14(4):334-341.
35. Chao P.H., Yodmuang S., Wang X., Sun L., Kaplan D.L., Vunjak-Novakovic G. Silk hydrogel for cartilage tissue engineering. J Biomed Mater Res B Appl Biomater 2010, 95(1):84-90.
36. Silva S.S., Motta A., Rodrigues M.T., Pinheiro A.F., Gomes M.E., Mano J.F., Reis R.L., Migliaresi C. Novel genipin-cross-linked chitosan/silk fibroin sponges for cartilage engineering strategies. Biomacromolecules 2008, 9(10):2764-2774.
37. Cheon Y.W., Lee W.J., Baek H.S., Lee Y.D., Park J.C., Park Y.H., Ki C.S., Chung K.H., Rah D.K. Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma. Artif Organs 2010, 34(5):384-392.
38. Talukdar S., Nguyen Q.T., Chen A.C., Sah R.L., Kundu S.C. Effect of initial cell seeding density on 3D-engineered silk fibroin scaffolds for articular cartilage tissue engineering. Biomaterials 2011, 32(34):8927-8937.
39. Bhardwaj N., Nguyen Q.T., Chen A.C., Kaplan D.L., Sah R.L., Kundu S.C. Potential of 3-D tissue constructs engineered from bovine chondrocytes/silk fibroin-chitosan for in vitro cartilage tissue engineering. Biomaterials 2011, 32(34):8927-8937.
40. Bhardwaj N., Kundu S.C. Chondrogenic differentiation of rat MSCs on porous scaffolds of silk fibroin/chitosan blends. Biomaterials 2012, 33(10):2848-2857.
41. Ni Y., Zhao X., Zhou L., Shao Z., Yan W., Chen X., Cao Z., Xue Z., Jiang J.J. Radiologic and histologic characterization of silk fibroin as scaffold coating for rabbit tracheal defect repair. Otolaryngol Head Neck Surg 2008, 139(2):256-261.
42. Zang M., Zhang Q., Davis G., Huang G., Jaffari M., Ríos C.N., Gupta V., Yu P., Mathur A.B. Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation. Acta Biomater 2011, 7(9):3422-3431.
43. Chang G., Kim H.J., Kaplan D., Vunjak-Novakovic G., Kandel R.A. Porous silk scaffolds can be used for tissue engineering annulus fibrosus. Eur Spine J. 2007, 16(11):1848-1857.
44. Chang G., Kim H.J., Vunjak-Novakovic G., Kaplan D.L., Kandel R. Enhancing annulus fibrosus tissue formation in porous silk scaffolds. J Biomed Mater Res A 2010, 92(1):43-51.
45. Park S.H., Gil E.S., Mandal B.B., Cho H., Kluge J.A., Min B.H., Kaplan D.L. Annulus fibrosus tissue engineering using lamellar silk scaffolds. J Tissue Eng Regen Med. 2012, 10.1002/term.541.
46. See E.Y., Toh S.L., Goh J.C. Simulated intervertebral disc-like assembly using bone marrow-derived mesenchymal stem cell sheets and silk scaffolds for annulus fibrosus regeneration. J Tissue Eng Regen Med 2012, 6(7):528-535. 10.1002/term.457.
47. Hu J., Chen B., Guo F., Du J., Gu P., Lin X., Yang W., Zhang H., Lu M., Huang Y., Xu G. Injectable silk fibroin/polyurethane composite hydrogel for nucleus pulposus replacement. J Mater Sci Mater Med 2012, 23(3):711-722.
48. Park S.H., Gil E.S., Cho H., Mandal B.B., Tien L.W., Min B.H., Kaplan D.L. Intervertebral disc tissue engineering using biphasic silk composite scaffolds. Tissue Eng Part A 2012, 18(5-6):447-458.
49. Yan L.P., Oliveira J.M., Oliveira A.L., Caridade S.G., Mano J.F., Reis R.L. Macro/microporous silk fibroin scaffolds with potential for articular cartilage and meniscus tissue engineering applications. Acta Biomater 2012, 8(1):289-301.
50. Mandal B.B., Park S.H., Gil E.S., Kaplan D.L. Stem cell-based meniscus tissue engineering. Tissue Eng Part A 2011, 17(21-22):2749-2761.
51. Mandal B.B., Park S.H., Gil E.S., Kaplan D.L. Multilayered silk scaffolds for meniscus tissue engineering. Biomaterials 2011, 32(2):639-651.
52. Yuksel E., Choo J., Wettergreen M., Liebschner M. Challenges in soft tissue engineering. Semin Plast Surg 2005, 19(3):261-270.
53. Altman G.H., Horan R.L., Lu H.H., Moreau J., Martin I., Richmond J.C., Kaplan D.L. Silk matrix for tissue engineered anterior cruciate ligaments. Biomaterials 2002, 23(20):4131-4141.
54. Altman G.H., Lu H.H., Horan R.L., Calabro T., Ryder D., Kaplan D.L., Stark P., Martin I., Richmond J.C., Vunjak-Novakovic G. Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineering. J Biomech Eng. 2002, 124(6):742-749.
55. Chen J., Altman G.H., Karageorgiou V., Horan R., Collette A., Volloch V., Colabro T., Kaplan D.L. Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers. J Biomed Mater Res A 2003, 67(2):559-570.
56. Kardestuncer T., McCarthy M.B., Karageorgiou V., Kaplan D., Gronowicz G. RGD-tethered silk substrate stimulates the differentiation of human tendon cells. Clin Orthop Relat Res. 2006, 448:234-239.
57. Liu H., Ge Z., Wang Y., Toh S.L., Sutthikhum V., Goh J.C. Modification of sericin-free silk fibers for ligament tissue engineering application. J Biomed Mater Res B Appl Biomater 2007, 82(1):129-138.
58. Fan H., Liu H., Wang Y., Toh S.L., Goh J.C. Development of a silk cable-reinforced gelatin/silk fibroin hybrid scaffold for ligament tissue engineering. Cell Transplant 2008, 17(12):1389-1401.
59. Fan H., Liu H., Toh S.L., Goh J.C. Enhanced differentiation of mesenchymal stem cells co-cultured with ligament fibroblasts on gelatin/silk fibroin hybrid scaffold. Biomaterials 2008, 29(8):1017-1027.
60. Chen X., Qi Y.Y., Wang L.L., Yin Z., Yin G.L., Zou X.H., Ouyang H.W. Ligament regeneration using a knitted silk scaffold combined with collagen matrix. Biomaterials 2008, 29(27):3683-3692.
61. Seo Y.K., Yoon H.H., Song K.Y., Kwon S.Y., Lee H.S., Park Y.S., Park J.K. Increase in cell migration and angiogenesis in a composite silk scaffold for tissue-engineered ligaments. J Orthop Res. 2009, 27(4):495-503.
62. Liu H., Fan H., Wang Y., Toh S.L., Goh J.C. The interaction between a combined knitted silk scaffold and microporous silk sponge with human mesenchymal stem cells for ligament tissue engineering. Biomaterials 2008, 29(6):662-674.
63. Bosetti M., Boccafoschi F., Calarco A., Leigheb M., Gatti S., Piffanelli V., Peluso G., Cannas M. Behaviour of human mesenchymal stem cells on a polyelectrolyte-modified HEMA hydrogel for silk-based ligament tissue engineering. J Biomater Sci Polym Ed. 2008, 19(9):1111-1123.
64. Fan H., Liu H., Wong E.J., Toh S.L., Goh J.C. In vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold. Biomaterials 2008, 29(23):3324-3337.
65. Fan H., Liu H., Toh S.L., Goh J.C. Anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold in large animal model. Biomaterials 2009, 30(28):4967-4977.
66. Horan R.L., Toponarski I., Boepple H.E., Weitzel P.P., Richmond J.C., Altman G.H. Design and characterization of a scaffold for anterior cruciate ligament engineering. J Knee Surg. 2009, 22(1):82-92.
67. Shen W., Chen X., Chen J., Yin Z., Heng B.C., Chen W., Ouyang H.W. The effect of incorporation of exogenous stromal cell-derived factor-1 alpha within a knitted silk-collagen sponge scaffold on tendon regeneration. Biomaterials 2010, 31(28):7239-7249.
68. Chen J.L., Yin Z., Shen W.L., Chen X., Heng B.C., Zou X.H., Ouyang H.W. Efficacy of hESC-MSCs in knitted silk-collagen scaffold for tendon tissue engineering and their roles. Biomaterials 2010, 31(36):9438-9451.
69. Fang Q., Chen D., Yang Z., Li M. In vitro and in vivo research on using Antheraea pernyi silk fibroin as tissue engineering tendon scaffolds. Mater Sci Eng C. 2009, 29:1527-1534.
70. Wu L., Li M., Zhao J., Chen D., Zhou Z. Preliminary study on polyvinyl alcohol/wild Antheraea pernyi silk fibroin as nanofiber scaffolds for tissue engineered tendon. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2011, 25(2):181-186.
71. Sahoo S., Toh S.L., Goh J.C. PLGA nanofiber-coated silk microfibrous scaffold for connective tissue engineering. J Biomed Mater Res B Appl Biomater 2010, 95(1):19-28.
72. Teh T.K., Toh S.L., Goh J.C. Aligned hybrid silk scaffold for enhanced differentiation of mesenchymal stem cells into ligament fibroblasts. Tissue Eng Part C Methods 2011, 17(6):687-703.
73. Sell S.A., McClure M.J., Ayres C.E., Simpson D.G., Bowlin G.L. Preliminary investigation of airgap electrospun silk-fibroin-based structures for ligament analogue engineering. J Biomater Sci Polym Ed. 2010, 22(10):1253-1273.
74. Chen K., Sahoo S., He P., Ng K.S., Toh S.L., Goh J.C. A hybrid silk/RADA-based fibrous scaffold with triple hierarchy for ligament regeneration. Tissue Eng Part A 2012, 18(13-14):1399-1409.
75. He P., Sahoo S., Ng K.S., Chen K., Toh S.L., Goh J.C. Enhanced osteoinductivity and osteoconductivity through hydroxyapatite coating of silk-based tissue-engineered ligament scaffold. J Biomed Mater Res A. 2012, 101A:555-566.
76. He P., Ng K.S., Toh S.L., Goh J.C. In vitro ligament-bone interface regeneration using a trilineage coculture system on a hybrid silk scaffold. Biomacromolecules 2012, 13(9):2692-2703.
77. Bi Y., Ehirchiou D., Kilts T.M., Inkson C.A., Embree M.C., Sonoyama W., Li L., Leet A.I., Seo B.M., Zhang L., Shi S., Young M.F. Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche. Nat Med 2007, 13(10):1219-1227.
78. Shen W., Chen J., Yin Z., Chen X., Liu H., Heng B.C., Chen W., Ouyang H.W. Allogenous tendon stem/progenitor cells in silk scaffold for functional shoulder repair. Cell Transplant 2012, 21(5):943-958.
79. Etienne O., Schneider A., Kluge J.A., Bellemin-Laponnaz C., Polidori C., Leisk G.G., Kaplan D.L., Garlick J.A., Egles C. Soft tissue augmentation using silk gels: an in vitro and in vivo study. J Periodontol 2009, 80(11):1852-1858.
80. Duan X., Tu Q., Zhang J., Ye J., Sommer C., Mostoslavsky G., Kaplan D., Yang P., Chen J. Application of induced pluripotent stem (iPS) cells in periodontal tissue regeneration. J Cell Physiol. 2011, 226(1):150-157.
81. Stegemann J.P., Kaszuba S.N., Rowe S.L. Review: advances in vascular tissue engineering using protein-based biomaterials. Tissue Eng. 2007, 13(11):2601-2613.
82. Nerem R.M., Seliktar D. Vascular tissue engineering. Annu Rev Biomed Eng. 2001, 3:225-243.
83. Unger R.E., Peters K., Wolf M., Motta A., Migliaresi C., Kirkpatrick C.J. Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of human endothelial cells. Biomaterials 2004, 25(21):5137-5146.
84. Ma M.L., Cao C.B., Li J.H., Zhu H.S. Novel prosthesis using silk fibroin for small caliber vascular. ASBM6: Advanced Biomaterials VI Book Series: Key Engineering Materials. 2005, 288-289:461-464.
85. Liu H., Li X., Niu X., Zhou G., Li P., Fan Y. Improved hemocompatibility and endothelialization of vascular grafts by covalent immobilization of sulfated silk fibroin on poly(lactic-co-glycolic acid) scaffolds. Biomacromolecules 2011, 12(8):2914-2924.
86. Liu H., Li X., Zhou G., Fan H., Fan Y. Electrospun sulfated silk fibroin nanofibrous scaffolds for vascular tissue engineering. Biomaterials 2011, 32(15):3784-3793.
87. Saitow C., Kaplan D.L., Castellot J.J. Heparin stimulates elastogenesis: application to silk-based vascular grafts. Matrix Biol. 2011, 30(5-6):346-355.
88. Enomoto S., Sumi M., Kajimoto K., Nakazawa Y., Takahashi R., Takabayashi C., Asakura T., Sata M. Long-term patency of small-diameter vascular graft made from fibroin, a silk-based biodegradable material. J Vasc Surg. 2010, 51(1):155-164.
89. Huang F., Sun L., Zheng J. In vitro and in vivo characterization of a silk fibroin-coated polyester vascular prosthesis. Artif Organs 2008, 32(12):932-941.
90. Lovett M.L., Cannizzaro C.M., Vunjak-Novakovic G., Kaplan D.L. Gel spinning of silk tubes for tissue engineering. Biomaterials 2008, 29(35):4650-4657.
91. Lovett M., Cannizzaro C., Daheron L., Messmer B., Vunjak-Novakovic G., Kaplan D.L. Silk fibroin microtubes for blood vessel engineering. Biomaterials 2007, 28(35):5271-5279.
92. Min S., Gao X., Liu L., Tian L., Zhu L., Zhang H., Yao J. Fabrication and characterization of porous tubular silk fibroin scaffolds. J Biomater Sci Polym Ed. 2009, 20(13):1961-1974.
93. Lovett M., Eng G., Kluge J.A., Cannizzaro C., Vunjak-Novakovic G., Kaplan D.L. Tubular silk scaffolds for small diameter vascular grafts. Organogenesis 2010, 6(4):217-224.
94. Zhao H.J. Prediction of mechanical properties of braided silk fibroin tubes for small diameter vascular scaffolds: a preliminary study. Int J Nonlin Sci Num Simulation 2010, 11 S:317-319.
95. Nakazawa Y., Sato M., Takahashi R., Aytemiz D., Takabayashi C., Tamura T., Enomoto S., Sata M., Asakura Development of small-diameter vascular grafts based on silk fibroin fibers from Bombyx mori for vascular regeneration. J Biomater Sci Polym Ed. 2010, 22(1-3):195-206.
96. Zhang X., Baughman C.B., Kaplan D.L. In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth. Biomaterials 2008, 29(14):2217-2227.
97. Soffer L., Wang X., Zhang X., Kluge J., Dorfmann L., Kaplan D.L., Leisk G. Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts. J Biomater Sci Polym Ed. 2008, 19(5):653-664.
98. Marelli B., Alessandrino A., Farè S., Freddi G., Mantovani D., Tanzi M.C. Compliant electrospun silk fibroin tubes for small vessel bypass grafting. Acta Biomater 2010, 6(10):4019-4026.
99. Wang S.D., Zhang Y.Z., Yin G.B., Wang H.W., Dong Z.H. Fabrication of a composite vascular scaffold using electrospinning technology. Mater Sci Eng C 2010, 30:670-676.
100. Zhou J., Cao C., Ma X., Lin J. Electrospinning of silk fibroin and collagen for vascular tissue engineering. Int J Biol Macromol 2010, 47(4):514-519.
101. Zhang K., Wang H., Huang C., Su Y., Mo X., Ikada Y. Fabrication of silk fibroin blended P(LLA-CL) nanofibrous scaffolds for tissue engineering. J Biomed Mater Res A 2010, 93(3):984-993.
102. McClure M.J., Sell S.A., Ayres C.E., Simpson D.G., Bowlin G.L. Electrospinning- aligned and random polydioxanone polycaprolactone- silk fibroin-blended scaffolds: geometry for a vascular matrix. Biomed Mater 2009, 4(5):055010.
103. Wang S.D., Zhang Y.Z., Yin G.B., Wang H.W., Dong Z.H. Electrospun polylactide/silk fibroin-gelatin composite tubular scaffolds for small-diameter tissue engineering blood vessels. J Appl Polym Sci. 2009, 113:2675-2682.
104. Lu Q., Hu K., Feng Q.L., Cui F. Growth of fibroblast and vascular smooth muscle cells in fibroin/collagen scaffold. Mater Sci Eng C. 2009, 29:2239-2245.
105. Unger R.E., Ghanaati S., Orth C., Sartoris A., Barbeck M., Halstenberg S., Motta A., Migliaresi C., Kirkpatrick C.J. The rapid anastomosis between prevascularized networks on silk fibroin scaffolds generated in vitro with cocultures of human microvascular endothelial and osteoblast cells and the host vasculature. Biomaterials 2010, 31(27):6959-6967.
106. Zhang X., Wang X., Keshav V., Wang X., Johanas J.T., Leisk G.G., Kaplan D.L. Dynamic culture conditions to generate silk-based tissue-engineered vascular grafts. Biomaterials 2009, 30(19):3213-3223.
107. Schmidt C.E., Leach J.B. Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng. 2003, 5:293-347.
108. Lu Y., Chi F.L., Zhao X., Shao Z.Z., Cao Z.B. Experimental study on facial nerve regeneration by porous silk fibroin conduit. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2006, 41(8):603-606.
109. Yang Y., Chen X., Ding F., Zhang P., Liu J., Gu X. Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro. Biomaterials 2007, 28(9):1643-1652.
110. Chen X.M., Yang Y.M., Wu J., Zhao Y.H., Ding F., Gu X.S. Biocompatibility studies of silk fibroin-based artificial nerve grafts in vitro and in vivo. Prog Nat Sci. 2007, 17:1029-1034.
111. Yang Y., Ding F., Wu J., Hu W., Liu W., Liu J., Gu X. Development and evaluation of silk fibroin-based nerve grafts used for peripheral nerve regeneration. Biomaterials 2007, 28(36):5526-5535.
112. Yang Y., Zhao Y., Gu Y., Yan X., Liu J., Ding F., Gu X. Degradation behaviors of nerve guidance conduits made up of silk fibroin in vitro and in vivo. Polym Degrad Stabil. 2009, 94:2213-2220.
113. Hu A.J., Zuo B.Q., Zhang F., Lan Q., Zhang H.X. Electrospun silk fibroin nanofibers promote Schwann cell adhesion, growth and proliferation. Neural Regen Res. 2012, 7:1171-1178.
114. Madduri S., Papaloïzos M., Gander B. Trophically and topographically functionalized silk fibroin nerve conduits for guided peripheral nerve regeneration. Biomaterials 2010, 31(8):2323-2334.
115. Wei Y., Gong K., Zheng Z., Wang A., Ao Q., Gong Y., Zhang X. Chitosan/silk fibroin-based tissue-engineered graft seeded with adipose-derived stem cells enhances nerve regeneration in a rat model. J Mater Sci Mater Med. 2011, 22(8):1947-1964.
116. Yang Y., Yuan X., Ding F., Yao D., Gu Y., Liu J., Gu X. Repair of rat sciatic nerve gap by a silk fibroin-based scaffold added with bone marrow mesenchymal stem cells. Tissue Eng Part A 2011, 17(17-18):2231-2244.
117. Wang J., Ye R., Wei Y., Wang H., Xu X., Zhang F., Qu J., Zuo B., Zhang H. The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells. J Biomed Mater Res A 2012, 100(3):632-645.
118. Rao J.W., Quan D.P., Peng L.M., Xie G.Y., Xu Y.B. The fabrication and characterization of linearly oriented lamellar-like multiple-channel silk fibroin nerve conduits. Biomed Eng-App Bas Commun. 2011, 23:403-417.
119. Qian Y., Shen Y., Lu Z., Fan Z., Liu T., Zhang J., Zhang F. Biocompatibility of silk fibroin nanofibers scaffold with olfactory ensheathing cells. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009, 23(11):1365-1370.
120. Shen Y., Qian Y., Zhang H., Zuo B., Lu Z., Fan Z., Zhang P., Zhang F., Zhou C. Guidance of olfactory ensheathing cell growth and migration on electrospun silk fibroin scaffolds. Cell Transplant 2010, 19(2):147-157.
121. Kim H.L., Yoo H., Park H.J., Kim Y.G., Lee D., Kang Y.S., Khang G. Effect of PLGA/silk fibroin hybrid film on attachment and proliferation of Schwann cells. Polymer-Korea 2011, 35:7-12.
122. Li S., Wu H., Hu X.D., Tu C.Q., Pei F.X., Wang G.L., Lin W., Fan H.S. Preparation of electrospun PLGA-silk fibroin nanofibers-based nerve conduits and evaluation in vivo. Artif Cells Blood Substit Immobil Biotechnol 2012, 40(1-2):171-178.
123. Wang C.Y., Zhang K.H., Fan C.Y., Mo X.M., Ruan H.J., Li F.F. Aligned natural-synthetic polyblend nanofibers for peripheral nerve regeneration. Acta Biomater 2011, 7(2):634-643.
124. Wang G., Hu X., Lin W., Dong C., Wu H. Electrospun PLGA-silk fibroin-collagen nanofibrous scaffolds for nerve tissue engineering. In vitro Cell Dev Biol Anim. 2011, 47(3):234-240.
125. Yang Y., Yuan X., Ding F., Yao D., Gu Y., Liu J., Gu X. Repair of rat sciatic nerve gap by a silk fibroin-based scaffold added with bone marrow mesenchymal stem cells. Tissue Eng Part A 2011, 17(17-18):2231-2244.
126. Zhang Q., Zhao Y., Yan S., Yang Y., Zhao H., Li M., Lu S., Kaplan D.L. Preparation of uniaxial multichannel silk fibroin scaffolds for guiding primary neurons. Acta Biomater 2012, 8(7):2628-2638.
127. Huang W., Begum R., Barber T., Ibba V., Tee N.C., Hussain M., Arastoo M., Yang Q., Robson L.G., Lesage S., Gheysens T., Skaer N.J., Knight D.P., Priestley J.V. Regenerative potential of silk conduits in repair of peripheral nerve injury in adult rats. Biomaterials 2012, 33(1):59-71.
128. Lin Y.C., Ramadan M., Hronik-Tupaj M., Kaplan D.L., Philips B.J., Sivak W., Rubin J.P., Marra K.G. Spatially controlled delivery of neurotrophic factors in silk fibroin-based nerve conduits for peripheral nerve repair. Ann Plast Surg 2011, 67(2):147-155.
129. Wittmer C.R., Claudepierre T., Reber M., Wiedemann P., Garlick J.A., Kaplan D., Egles C. Multifunctionalized electrospun silk fibers promote axon regeneration in central nervous system. Adv Funct Mater 2011, 21(22):4202.
130. Tang X., Xue C., Wang Y., Ding F., Yang Y., Gu X. Bridging peripheral nerve defects with a tissue engineered nerve graft composed of an in vitro cultured nerve equivalent and a silk fibroin-based scaffold. Biomaterials 2012, 33(15):3860-3867.
131. Wang J., Ye R., Wei Y., Wang H., Xu X., Zhang F., Qu J., Zuo B., Zhang H. The effects of electrospun TSF nanofiber diameter and alignment on neuronal differentiation of human embryonic stem cells. J Biomed Mater Res A 2012, 100(3):632-645.
132. Xia Y., Lu Y. Fabrication and properties of conductive conjugated polymers/silk fibroin composite fibers. Compos Sci Technol. 2008, 68:1471-1479.
133. Aznar-Cervantes S., Roca M.I., Martinez J.G., Meseguer-Olmo L., Cenis J.L., Moraleda J.M., Otero T.F. Fabrication of conductive electrospun silk fibroin scaffolds by coating with polypyrrole for biomedical applications. Bioelectrochemistry. 2012, 85:36-43.
134. Chen C.S., Soni S., Le C., Biasca M., Farr E., Chen E.Y., Chin W.C. Human stem cell neuronal differentiation on silk-carbon nanotube composite. Nanoscale Res Lett. 2012, 7(1):126.
135. Ghasemi-Mobarakeh L., Prabhakaran M.P., Morshed M., Nasr-Esfahani M.H., Ramakrishna S. Electrical stimulation of nerve cells using conductive nanofibrous scaffolds for nerve tissue engineering. Tissue Eng Part A 2009, 15(11):3605-3619.
136. Sugihara A., Sugiura K., Morita H., Ninagawa T., Tubouchi K., Tobe R., Izumiya M., Horio T., Abraham N.G., Ikehara S. Promotive effects of a silk film on epidermal recovery from full-thickness skin wounds. Proc Soc Exp Biol Med. 2000, 225(1):58-64.
137. Roh D.H., Kang S.Y., Kim J.Y., Kwon Y.B., Young Kweon H., Lee K.G., Park Y.H., Baek R.M., Heo C.Y., Choe J., Lee J.H. Wound healing effect of silk fibroin/alginate-blended sponge in full thickness skin defect of rat. J Mater Sci Mater Med. 2006, 17(6):547-552.
138. Yoo C.R., Yeo I.S., Park K.E., Park J.H., Lee S.J., Park W.H., Min B.M. Effect of chitin/silk fibroin nanofibrous bicomponent structures on interaction with human epidermal keratinocytes. Int J Biol Macromol 2008, 42(4):324-334.
139. Altman A.M., Yan Y., Matthias N., Bai X., Rios C., Mathur A.B., Song Y.H., Alt E.U. IFATS collection: Human adipose-derived stem cells seeded on a silk fibroin-chitosan scaffold enhance wound repair in a murine soft tissue injury model. Stem Cells 2009, 27(1):250-258.
140. Luangbudnark W., Viyoch J., Laupattarakasem W., Surakunprapha P., Laupattarakasem P. Properties and biocompatibility of chitosan and silk fibroin blend films for application in skin tissue engineering. Sci World J. 2012, 2012:697201.
141. Inpanya P., Faikrua A., Ounaroon A., Sittichokechaiwut A., Viyoch J. Effects of the blended fibroin/aloe gel film on wound healing in streptozotocin-induced diabetic rats. Biomed Mater 2012, 7(3):035008.
142. Jeong L., Yeo I.S., Kim H.N., Yoon Y.I., Jang da H., Jung S.Y., Min B.M., Park W.H. Plasma-treated silk fibroin nanofibers for skin regeneration. Int J Biol Macromol 2009, 44(3):222-228.
143. Dal Pra I., Chiarini A., Boschi A., Freddi G., Armato U. Novel dermo-epidermal equivalents on silk fibroin-based formic acid-crosslinked three-dimensional nonwoven devices with prospective applications in human tissue engineering/regeneration/repair. Int J Mol Med. 2006, 18(2):241-247.
144. Schneider A., Wang X.Y., Kaplan D.L., Garlick J.A., Egles C. Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound healing. Acta Biomater 2009, 5(7):2570-2578.
145. Okabayashi R., Nakamura M., Okabayashi T., Tanaka Y., Nagai A., Yamashita K. Efficacy of polarized hydroxyapatite and silk fibroin composite dressing gel on epidermal recovery from full-thickness skin wounds. J Biomed Mater Res B Appl Biomater 2009, 90(2):641-646.
146. Kim D.W., Hwang H.S., Kim D.S., Sheen S.H., Heo D.H., Hwang G., Kang S.H., Kweon H., Jo Y.Y., Kang S.W., Lee K.G., Park K.W., Han K.H., Park J., Eum W.S., Cho Y.J., Choi H.C., Choi S.Y. Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model. BMB Rep. 2011, 44(12):787-792.
147. Ikegawa Y., Sato S., Lim G., Hur W., Tanaka K., Komori M., Takenaka S., Taira T. Amelioration of the progression of an atopic dermatitis-like skin lesion by silk peptide and identification of functional peptides. Biosci Biotechnol Biochem 2012, 76(3):473-477.
148. Li H., Li L., Qian Y., Cai K., Lu Y., Zhong L., Liu W., Yang L. Preparation and cytocompatibility study of poly (epsilon-caprolactone)/silk sericin nanofibrous scaffolds. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2011, 28(2):305-309.
149. Nayak S., Talukdar S., Kundu S.C. Potential of 2D crosslinked sericin membranes with improved biostability for skin tissue engineering. Cell Tissue Res. 2012, 347(3):783-794.
150. Kundu B., Kundu S.C. Silk sericin/polyacrylamide in situ forming hydrogels for dermal reconstruction. Biomaterials 2012, 33(30):7456-7467.
151. Diekmann S., Bader A., Schmitmeier S. Present and future developments in hepatic tissue engineering for liver support systems: state of the art and future developments of hepatic cell culture techniques for the use in liver support systems. Cytotechnology 2006, 50(1-3):163-179.
152. Cirillo B., Morra M., Catapano G. Adhesion and function of rat liver cells adherent to silk fibroin/collagen blend films. Int J Artif Organs 2004, 27(1):60-68.
153. Gotoh Y., Niimi S., Hayakawa T., Miyashita T. Preparation of lactose-silk fibroin conjugates and their application as a scaffold for hepatocyte attachment. Biomaterials 2004, 25(6):1131-1140.
154. She Z., Jin C., Huang Z., Zhang B., Feng Q., Xu Y. Silk fibroin/chitosan scaffold: preparation, characterization, and culture with HepG2 cell. J Mater Sci Mater Med. 2008, 19(12):3545-3553.
155. Gotoh Y., Ishizuka Y., Matsuura T., Niimi S. Spheroid formation and expression of liver-specific functions of human hepatocellular carcinoma-derived FLC-4 cells cultured in lactose-silk fibroin conjugate sponges. Biomacromolecules 2011, 12(5):1532-1539.
156. Kasoju N., Bora U. Silk fibroin based biomimetic artificial extracellular matrix for hepatic tissue engineering applications. Biomed Mater 2012, 7(4):045004.
157. Wang X., Zhang X., Sun L., Subramanian B., Maffini M.V., Soto A., Sonnenschein C., Kaplan D.L. Preadipocytes stimulate ductal morphogenesis and functional differentiation of human mammary epithelial cells on 3D silk scaffolds. Tissue Eng Part A 2009, 15(10):3087-3098.
158. Wang X., Sun L., Maffini M.V., Soto A., Sonnenschein C., Kaplan D.L. A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function. Biomaterials 2010, 31(14):3920-3929.
159. Gupta V., Mun G.H., Choi B., Aseh A., Mildred L., Patel A., Zhang Q., Price J.E., Chang D., Robb G., Mathur A.B. Repair and reconstruction of a resected tumor defect using a composite of tissue flap-nanotherapeutic-silk fibroin and chitosan scaffold. Ann Biomed Eng. 2011, 39(9):2374-2387.
160. Zhang Y., Zhou P., Pan L.F., Xie S.Z., Sun M., Li W.T. Growth of human smooth muscle cells on the silk fibroin modified-polyhydroxyalkanoate scaffold. Acta Chim Sinica. 2007, 65(24):2935-2940.
161. Yang M.C., Wang S.S., Chou N.K., Chi N.H., Huang Y.Y., Chang Y.L., Shieh M.J., Chung T.W. The cardiomyogenic differentiation of rat mesenchymal stem cells on silk fibroin-polysaccharide cardiac patches in vitro. Biomaterials 2009, 30(22):3757-3765.
162. Yang M.C., Chi N.H., Chou N.K., Huang Y.Y., Chung T.W., Chang Y.L., Liu H.C., Shieh M.J., Wang S.S. The influence of rat mesenchymal stem cell CD44 surface markers on cell growth, fibronectin expression, and cardiomyogenic differentiation on silk fibroin - Hyaluronic acid cardiac patches. Biomaterials 2010, 31(5):854-862.
163. Patra C., Talukdar S., Novoyatleva T., Velagala S.R., Mühlfeld C., Kundu B., Kundu S.C., Engel F.B. Silk protein fibroin from Antheraea mylitta for cardiac tissue engineering. Biomaterials 2012, 33(9):2673-2680.
164. Atala A. Tissue engineering of human bladder. Br Med Bull. 2011, 97:81-104.
165. Shimamura T. Growth promotion of urothelial cells by a silk suture in the bladder walls of rats. Acta Pathol Jpn. 1987, 37(2):273-280.
166. Liu C.X., Liao Y.F., Li H.L., Zheng S.B. Cytocompatibility of silk fibroin film with rabbit urinary bladder transitional epithelial cells in vitro. Nan Fang Yi Ke Da Xue Xue Bao. 2008, 28(2):216-218.
167. Liu C.X., Lin Y.Y., Li H.L., Zheng S.B. Application of silk fibroin film for repairing rabbit urethral defect. Nan Fang Yi Ke Da Xue Xue Bao. 2007, 27(2):184-187.
168. Mauney J.R., Cannon G.M., Lovett M.L., Gong E.M., Di Vizio D., Gomez P., Kaplan D.L., Adam R.M., Estrada C.R. Evaluation of gel spun silk-based biomaterials in a murine model of bladder augmentation. Biomaterials 2011, 32(3):808-818.
169. Gomez P., Gil E.S., Lovett M.L., Rockwood D.N., Di Vizio D., Kaplan D.L., Adam R.M., Estrada C.R., Mauney J.R. The effect of manipulation of silk scaffold fabrication parameters on matrix performance in a murine model of bladder augmentation. Biomaterials 2011, 32(30):7562-7570.
170. Zou X.H., Zhi Y.L., Chen X., Jin H.M., Wang L.L., Jiang Y.Z., Yin Z., Ouyang H.W. Mesenchymal stem cell seeded knitted silk sling for the treatment of stress urinary incontinence. Biomaterials 2010, 31(18):4872-4879.
171. Harkin D.G., George K.A., Madden P.W., Schwab I.R., Hutmacher D.W., Chirila T.V. Silk fibroin in ocular tissue reconstruction. Biomaterials 2011, 32(10):2445-2458.
172. Peyman G.A., Koziol J., Kaiserman R. Iris tissue reaction to dacron and silk in primates: evaluation for intraocular lens fixation. Ophthalmic Surg 1979, 10(1):69-75.
173. Chirila T., Barnard Z., Zainuddin, Harkin D.G., Schwab I.R., Hirst L. Bombyx mori silk fibroin membranes as potential substrata for epithelial constructs used in the management of ocular surface disorders. Tissue Eng Part A. 2008, 14(7):1203-1211.
174. Lawrence B.D., Marchant J.K., Pindrus M.A., Omenetto F.G., Kaplan D.L. Silk film biomaterials for cornea tissue engineering. Biomaterials 2009, 30(7):1299-1308.
175. Gil E.S., Park S.H., Marchant J., Omenetto F., Kaplan D.L. Response of human corneal fibroblasts on silk film surface patterns. Macromol Biosci. 2010, 10(6):664-673.
176. Gil E.S., Mandal B.B., Park S.H., Marchant J.K., Omenetto F.G., Kaplan D.L. Helicoidal multi-lamellar features of RGD-functionalized silk biomaterials for corneal tissue engineering. Biomaterials 2010, 31(34):8953-8963.
177. Madden P.W., Lai J.N., George K.A., Giovenco T., Harkin D.G., Chirila T.V. Human corneal endothelial cell growth on a silk fibroin membrane. Biomaterials 2011, 32(17):4076-4084.
178. Higa K., Takeshima N., Moro F., Kawakita T., Kawashima M., Demura M., Shimazaki J., Asakura T., Tsubota K., Shimmura S. Porous silk fibroin film as a transparent carrier for cultivated corneal epithelial sheets. J Biomater Sci Polym Ed. 2010, 22:2261-2276.
179. Bray L.J., George K.A., Ainscough S.L., Hutmacher D.W., Chirila T.V., Harkin D.G. Human corneal epithelial equivalents constructed on Bombyx mori silk fibroin membranes. Biomaterials 2011, 32(22):5086-5091.
180. Bray L.J., George K.A., Hutmacher D.W., Chirila T.V., Harkin D.G. A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus. Biomaterials 2012, 33(13):3529-3538.
181. Shadforth A.M., George K.A., Kwan A.S., Chirila T.V., Harkin D.G. The cultivation of human retinal pigment epithelial cells on Bombyx mori silk fibroin. Biomaterials 2012, 33(16):4110-4117.
182. Ghassemifar R., Redmond S., Zainuddin, Chirila T.V. Advancing towards a tissue-engineered tympanic membrane: silk fibroin as a substratum for growing human eardrum keratinocytes. J Biomater Appl. 2010, 24(7):591-606.
183. Levin B., Redmond S.L., Rajkhowa R., Eikelboom R.H., Marano R.J., Atlas M.D. Preliminary results of the application of a silk fibroin scaffold to otology. Otolaryngol Head Neck Surg 2010, 142(3 Suppl 1):S33-S35.
184. Levin B., Redmond S.L., Rajkhowa R., Eikelboom R.H., Atlas M.D., Marano R.J. Utilising silk fibroin membranes as scaffolds for the growth of tympanic membrane keratinocytes, and application to myringoplasty surgery. J Laryngol Otol. 2012 Aug, 15:1-8.
185. Levin B., Rajkhowa R., Redmond S.L., Atlas M.D. Grafts in myringoplasty: utilizing a silk fibroin scaffold as a novel device. Expert Rev Med Devices 2009, 6(6):653-664.
186. Ubaldo A., Ilaria D.P., Anna C., Giuliano F. Will silk fibroin nanofiber scaffolds ever hold a useful place in Translational Regenerative Medicine?. Int J Burn Trauma. 2011, 1(1):27-33.