Fabrication of conductive electrospun silk fibroin scaffolds by coating with polypyrrole for biomedical applications

Bioelectrochemistry

Volumn 85, Issue , 2012, Pages 36-43

  Aznar Cervantes, Salvador ^a   Roca, Maria I ^b   Martinez, Jose G ^b   Meseguer Olmo, Luis ^c   Cenis, Jose L ^a   Moraleda, Jose M ^c   Otero, Toribio F ^b  

^a INSTITUTO MURCIANO DE INVESTIGACIÓN Y DESARROLLO AGRARIO Y ALIMENTARIO IMIDA   (Spain)

^b TECHNICAL UNIVERSITY OF CARTAGENA   (Spain)

^c HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

Author keywords

Adult human mesenchymal stem cells; Anion storage; Electroactivity; Fibroin polypyrrole meshes; Human fibroblast

Indexed keywords

BIOMEDICAL APPLICATIONS; CHEMICAL LINKAGE; CHEMICAL POLYMERIZATION; ELECTRO-ACTIVITY; ELECTROSPUNS; FIBROIN-POLYPYRROLE MESHES; HUMAN FIBROBLAST; HUMAN MESENCHYMAL STEM CELLS; MATERIALS SUPPORTS; MECHANICAL RESISTANCE; POLYPYRROLE COATINGS; SILK FIBROIN; STORAGE AND DELIVERY;

CELL CULTURE; COATED MATERIALS; COATINGS; FIBROBLASTS; MEDICAL APPLICATIONS; SILK; STEM CELLS;

POLYPYRROLES;

POLYPYRROLE; SILK FIBROIN;

AQUEOUS SOLUTION; ARTICLE; CELL ADHESION; CELL PROLIFERATION; ELECTROSPINNING; FIBROBLAST; HUMAN; HUMAN CELL; IN VITRO STUDY; INFRARED SPECTROSCOPY; MESENCHYMAL STEM CELL; OXIDATION REDUCTION REACTION; SCANNING ELECTRON MICROSCOPY;

CELL ADHESION; CELL PROLIFERATION; COATED MATERIALS, BIOCOMPATIBLE; FIBROBLASTS; FIBROINS; HUMANS; MESENCHYMAL STEM CELLS; OXIDATION-REDUCTION; POLYMERS; PYRROLES; SILK; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

ANIONS; BIOMETRICS; CELLS; COATINGS; FIBROIN; HUMAN ENGINEERING; MEDICINE; SILK;

EID: 84857356084     PISSN: 15675394     EISSN: 1878562X     Source Type: Journal    
DOI: 10.1016/j.bioelechem.2011.11.008     Document Type: Article

References (35)

1. Wang Y.Z., Kim H.J., Vunjak-Novakovic G., Kaplan D.L. Stem cell-based tissue engineering with silk biomaterials. Biomaterials 2006, 27:6064-6082.
2. Yang Y., Chen X., Ding F., Zhang P., Liu J., Gu X. Biocompatibility evaluation of silk fibroin with peripheral nerve tissues and cells in vitro. Biomaterials 2007, 28:1643-1652.
3. Soffer L., Wang X.Y., Mang X.H., Kluge J., Dorfmann L., Kaplan D.L., Leisk G. Silk-based electrospun tubular scaffolds for tissue-engineered vascular grafts. J. Biomater. Sci. Polym. Ed. 2008, 19:653-664.
4. Santin M., Motta A., Freddi G., Cannas M. In vitro evaluation of the inflammatory potential of the silk fibroin. J. Biomed. Mater. Res. 1999, 46:382-389.
5. Schneider A., Wang X.Y., Kaplan D.L., Garlick J.A., Egles C. Biofunctionalized electrospun silk mats as a topical bioactive dressing for accelerated wound heating. Acta Biomater. 2009, 5:2570-2578.
6. Zhang X.H., Baughman C.B., Kaplan D.L. In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth. Biomaterials 2008, 29:2217-2227.
7. Min B.M., Lee G., Kim S.H., Nam Y.S., Lee T.S., Park W.H. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 2004, 25:1289-1297.
8. Jin H.J., Chen J.S., Karageorgiou V., Altman G.H., Kaplan D.L. Human bone marrow stromal cell responses on electrospun silk fibroin mats. Biomaterials 2004, 25:1039-1047.
9. Mcleod K.J., Donahue H.J., Levin P.E., Fontaine M.A., Rubin C.T. Electric-fields modulate bone cell-function in a density-dependent manner. J. Bone Miner. Res. 1993, 8:977-984.
10. Baek H.S., Park Y.H., Ki C.S., Park J.C., Rah D.K. Enhanced chondrogenic responses of articular chondrocytes onto porous silk fibroin scaffolds treated with microwave-induced argon plasma. Surf. Coat. Technol. 2008, 202:5794-5797.
11. Zhang X., Reagan M.R., Kaplan D.L. Electrospun silk biomaterial scaffolds for regenerative medicine. Adv. Drug Deliv. Rev. 2009, 61:988-1006.
12. Hohman M.M., Shin M., Rutledge G., Brenner M.P. Electrospinning and electrically forced jets. I. Stability theory. Phys. Fluids 2001, 13:2201-2220.
13. Park K.E., Jung S.Y., Lee S.J., Min B.M., Park W.H. Biomimetic nanofibrous scaffolds: preparation and characterization of chitin/silk fibroin blend nanofibers. Int. J. Biol. Macromol. 2006, 38:165-173.
14. Yeo I.S., Oh J.E., Jeong L., Lee T.S., Lee S.J., Park W.H., Min B.M. Collagen-based biomimetic nanofibrous scaffolds: preparation and characterization of collagen/silk fibroin bicomponent nanofibrous structures. Biomacromolecules 2008, 9:1106-1116.
15. Wang M., Yu J.H., Kaplan D.L., Rutledge G.C. Production of submicron diameter silk fibers under benign processing conditions by two-fluid electrospinning. Macromolecules 2006, 39:1102-1107.
16. Li C.M., Vepari C., Jin H.J., Kim H.J., Kaplan D.L. Electrospun silk-BMP-2 scaffolds for bone tissue engineering. Biomaterials 2006, 27:3115-3124.
17. Liu H., Qian J., Liu Y., Yu T., Deng J. Immobilization of glucose oxidase in the composite membrane of regenerated silk fibroin and poly(vinyl alcohol): application to an amperometric glucose sensor. Bioelectrochem. Bioenerg. 1996, 39:303-308.
18. Guimard N.K., Gomez N., Schmidt C.E. Conducting polymers in biomedical engineering. Prog. Polym. Sci. 2007, 32:876-921.
19. Ravichandran R., Sundarrajan S., Venugopal J.R., Mukherjee S., Ramakrishna S. Applications of conducting polymers and their issues in biomedical engineering. J. R. Soc. Interface 2010, 7:S559-S579.
20. Chronakis I.S., Grapenson S., Jakob A. Conductive polypyrrole nanofibers via electrospinning: electrical and morphological properties. Polymer 2006, 47:1597-1603.
21. Lee J.Y., Bashur C.A., Goldstein A.S., Schmidt C.E. Polypyrrole-coated electrospun PLGA nanofibers for neural tissue applications. Biomaterials 2009, 30:4325-4335.
22. Collazos-Castro J.E., Polo J.L., Hernandez-Labrado G.R., Padial-Canete V., Garcia-Rama C. Bioelectrochemical control of neural cell development on conducting polymers. Biomaterials 2010, 31:9244-9255.
23. Akieh M.N., Price W.E., Bobacka J., Ivaska A., Ralph S.F. Ion exchange behaviour and charge compensation mechanism of polypyrrole in electrolytes containing mono-, di- and trivalent metal ions. Synth. Met. 2009, 159:2590-2598.
24. Otero T.F. Soft, wet, and reactive polymers. Sensing artificial muscles and conformational energy. J. Mater. Chem. 2009, 19:681-689.
25. Wang L.X., Li X.G., Yang Y.L. Preparation, properties and applications of polypyrroles. React. Funct. Polym. 2001, 47:125-139.
26. Rho K.S., Jeong L., Lee G., Seo B.M., Park Y.J., Hong S.D., Roh S., et al. Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 2006, 27:1452-1461.
27. Otero T.F., Ariza M.J. Revisiting the electrochemical and polymeric behavior of a polypyrrole free-standing electrode in aqueous solution. J. Phys. Chem. B 2003, 107:13954-13961.
28. Ren X.M., Pickup P.G. Ion-transport in polypyrrole and a polypyrrole polyanion composite. J. Phys. Chem. 1993, 97:5356-5362.
29. Otero T.F., Abadias R. Poly(3-methylthiophene) oxidation under chemical control. Rate coefficients change with prepolarization potentials of reduction. J. Electroanal. Chem. 2007, 610:96-101.
30. Otero T.F., Santos F. Polythiophene oxidation: rate coefficients, activation energy and conformational energies. Electrochim. Acta 2008, 53:3166-3174.
31. Otero T.F., de Otazo J.M.G. Polypyrrole oxidation: kinetic coefficients, activation energy and conformational energy. Synth. Met. 2009, 159:681-688.
32. Wang H., Shao H.L., Hu X.C. Structure of silk fibroin fibers made by an electrospinning process from a silk fibroin aqueous solution. J. Appl. Polym. Sci. 2006, 101:961-968.
33. Xia Y.Y., Lu Y. Fabrication and properties of conductive conjugated polymers/silk fibroin composite fibers. Compos. Sci. Technol. 2008, 68:1471-1479.
34. Cucchi I., Boschi A., Arosio C., Bertini F., Freddi G., Catellani M. Bio-based conductive composites: preparation and properties of polypyrrole (PPy)-coated silk fabrics. Synth. Met. 2009, 159:246-253.
35. Meinel A.J., Kubow K.E., Klotzsch E., Garcia-Fuentes M., Smith M.L., Vogel V., Merkle H.P., et al. Optimization strategies for electrospun silk fibroin tissue engineering scaffolds. Biomaterials 2009, 30:3058-3067.