An in vitro blood-brain barrier model combining shear stress and endothelial cell/astrocyte co-culture

Journal of Neuroscience Methods

Volumn 232, Issue , 2014, Pages 165-172

  Takeshita, Yukio ^a   Obermeier, Birgit ^a   Cotleur, Anne ^a   Sano, Yasuteru ^b   Kanda, Takashi ^b   Ransohoff, Richard M ^a  

^a LERNER RESEARCH INSTITUTE   (United States)

^b YAMAGUCHI UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

Blood brain barrier; Co culture; Human astrocyte cell line; Human endothelial cell line; In vitro model; Leukocyte transmigration; Migration assay; Shear forces

Indexed keywords

CD14 ANTIGEN; CD19 ANTIGEN; CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; CLAUDIN 5; GAMMA INTERFERON; INTERCELLULAR ADHESION MOLECULE 1; OCCLUDIN; TUMOR NECROSIS FACTOR ALPHA; VIRUS LARGE T ANTIGEN; GLIAL FIBRILLARY ACIDIC PROTEIN; PROTEIN ZO1; TJP1 PROTEIN, HUMAN; VON WILLEBRAND FACTOR;

ADULT; ARTICLE; ASTROCYTE; ASTROCYTE CULTURE; BLOOD BRAIN BARRIER; CELL FUNCTION; CELL MEMBRANE PERMEABILITY; CELL MIGRATION; COCULTURE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INTERMETHOD COMPARISON; LEUKOCYTE; NORMAL HUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SHEAR STRESS; TIGHT JUNCTION; BIOLOGICAL MODEL; CELL CULTURE; CYTOLOGY; FEMALE; GENE EXPRESSION REGULATION; MALE; MECHANICAL STRESS; METABOLISM; MICROVASCULATURE; MIDDLE AGED; PHYSIOLOGY; TEMPERATURE; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION; TRANSPORT AT THE CELLULAR LEVEL; ULTRASTRUCTURE; YOUNG ADULT;

ADULT; ASTROCYTES; BIOLOGICAL TRANSPORT; BLOOD-BRAIN BARRIER; CELLS, CULTURED; CLAUDIN-5; COCULTURE TECHNIQUES; ENDOTHELIAL CELLS; FEMALE; GENE EXPRESSION REGULATION; GLIAL FIBRILLARY ACIDIC PROTEIN; HUMANS; MALE; MICROVESSELS; MIDDLE AGED; MODELS, BIOLOGICAL; OCCLUDIN; STRESS, MECHANICAL; TEMPERATURE; TRANSENDOTHELIAL AND TRANSEPITHELIAL MIGRATION; VON WILLEBRAND FACTOR; YOUNG ADULT; ZONULA OCCLUDENS-1 PROTEIN;

EID: 84902212476     PISSN: 01650270     EISSN: 1872678X     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2014.05.013     Document Type: Article

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