Simplified 2,4-dinitrophenylhydrazine spectrophotometric assay for quantification of carbonyls in oxidized proteins

Analytical Biochemistry

Volumn 458, Issue , 2014, Pages 69-71

  Mesquita, Cristina S ^a   Oliveira, Raquel ^a,b   Bento, Fátima ^a   Geraldo, Dulce ^a   Rodrigues, João V ^a,c   Marcos, João C ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b UNIVERSITÉ PARIS DIDEROT   (France)

^c INSTITUTO DE BIOLOGIA EXPERIMENTAL E TECNOLÓGICA   (Portugal)

Author keywords

Carbonyl quantification; DNPH assay; Oxidative stress; Protein oxidation

Indexed keywords

OXIDATION; OXIDATIVE STRESS; SODIUM HYDROXIDE; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY;

2 ,4-DINITROPHENYLHYDRAZINE; CARBONYL GROUPS; CARBONYL QUANTIFICATION; MAXIMUM ABSORBANCE; PROTEIN OXIDATION; PROTEIN SOLUTION; SAMPLE SOLUTION; SPECTROPHOTOMETRIC ASSAYS;

PROTEINS;

2,4 DINITROPHENYLHYDRAZINE; CARBONYL DERIVATIVE; OXIDIZED PROTEIN; PROTEIN; SODIUM HYDROXIDE; UNCLASSIFIED DRUG; 2,4-DINITROPHENYLHYDRAZINE; PHENYLHYDRAZINE DERIVATIVE;

ARTICLE; FENTON REACTION; PRECIPITATION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPECTROPHOTOMETRY; SUSPENSION; CHEMISTRY; METABOLISM; OXIDATION REDUCTION REACTION;

OXIDATION-REDUCTION; PHENYLHYDRAZINES; PROTEINS; SODIUM HYDROXIDE; SPECTROPHOTOMETRY;

EID: 84901936614     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.04.034     Document Type: Article

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