Standardization of a protocol to extract and analyze chlorophyll a and carotenoids in Gracilaria tenuistipitata var. liui. zhang and xia (rhodophyta)

Brazilian Journal of Oceanography

Volumn 62, Issue 1, 2014, Pages 57-63

  Torres, Priscila Bezerra ^a   Chow, Fungyi ^a   Furlan, Cláudia Maria ^b   Mandelli, Fernanda ^b   Mercadante, Adriana ^b   dos Santos, Déborah Yara Alves Cursino ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

Carotenoids; Chlorophyll a; Gracilaria tenuistipitata; HPLC; UV visible light spectrophotometry

Indexed keywords

EID: 84901934718     PISSN: 16798759     EISSN: 1982436X     Source Type: Journal    
DOI: 10.1590/S1679-87592014068106201     Document Type: Article

References (23)

1. ARVAYO-ENRÍQUEZ, H.; MONDACA-FERNÁNDEZ, I.; GORTÁREZ-MOROYOQUI, P.; LÓPEZ-CERVANTES, J.; RODRÍGUEZ-RAMÍREZ, R. Carotenoids extraction and quantification: a review. Anal. Methods, v. 5, p. 2916-2924, 2013.
2. BORSARELLI, C.; MERCADANTE, A. Thermal and photochemical degradation of carotenoids. In: LANDRUM, J. T. (Ed.). Carotenoids: physical, chemical, and biological functions and properties. Boca Raton: Taylor & Francis Group, 2010. p. 229-253.
3. CARNICAS, E.; JIMÉNEZ, C.; NIELL, F. X. Effects of changes of irradiance on the pigment composition of Gracilaria tenuistipitata var. liui Zhang et Xia. J. Photochem. Photobiol., B, v. 50, n. 2/3, p. 149-158, 1999.
4. EDWARDS, P. Illustrated guide to the seaweeds and sea grasses in the vicinity of Porto Aransas, Texas. Contrib. Mar. Sci., v. 15, p. 1-228, 1970.
5. FARIA, A. F.; HASEGAWA, P. N.; CHAGAS, E. A.; PIO, R.; PURGATTO, E.; MERCADANTE, A. Z. Cultivar influence on carotenoid composition of loquats from Brazil. J. Food Compos. Anal., v. 22, n. 3, p. 196-203, 2009.
6. GAUTHIER-JAQUES, A.; BORTLIK, K.; HAU, J.; FAY, L. B. Improved method to track chlorophyll degradation. J. Agric. Food Chem., v. 49, n. 3, p. 1117-1122, 2001.
7. GUEDES, A. C.; AMARO, H. M.; MALCATA, F. X. Microalgae as sources of carotenoids. Mar. Drugs, v. 9, n. 4, p. 625-644, 2011.
8. ISHIDA, B. K.; CHAPMAN, M. H. Carotenoid extraction from plants using a novel, environmentally friendly solvent. J. Agric. Food Chem., v. 57, n. 3, p. 1051-1059, 2009.
9. JEFFREY, S. W.; HUMPHREY, G. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanz., v. 649, n. 1, p. 105-149, 1975.
10. KELMAN, D.; POSNER, E. K.; MCDERMID, K. J.; TABANDERA, N. K.; WRIGHT, P. R.; WRIGHT, A. D. Antioxidant activity of Hawaiian marine algae. Mar. Drugs, v. 10, n. 2, p. 403-16, 2012.
11. KHACHIK, F. Analysis of carotenoids in nutritional studies. In BRITTON, G.; LIAAEN-JENSEN, S.; PFANDER, D. H. (Eds.). Nutrition and health. Basel: Boston: Berlin: Birkhäuser, 2009. p. 7-44. (Carotenoids; v. 5).
12. KOPEC, R. E.; COOPERSTONE, J. L.; CICHON, M. J.; SCHWARTZ, S. J. Analysis methods of carotenoids. In: XU, Z.; HOWARD, L. R. (Eds.). Analysis of antioxidant-rich phytochemicals. Hoboken: Chichester: Wiley-Blackwell, 2012. p.105-149.
13. KOPECKY, J.; SCHOEFS, B.; LOEST, K.; STYS, D.; PULZ, O. Microalgae as a source for secondary carotenoid production: a screening study. Arch. Hydrobiol., Suppl., v. 133, p. 153-168, 2000.
14. LICHTENTHALER, H. K.; BUSCHMANN, C. Chlorophylls and carotenoids: measurement and characterization by UV-VIS. In: WROLSTAD, R. E.; ACREE, T. E.; DECKER, E. A.; PENNER, M. H.; REID, D. S.; SCHWARTZ, S. J.; SHOEMAKER, C. F.; SPORNS, P. Current protocols in food analytical chemistry. New York: John Wiley, 2001. p. F4.3.1-F4.3.8.
15. MARINHO-SORIANO, E. Effect of depth on growth and pigment contents of the macroalgae Gracilaria bursa-pastoris. Rev. Bras. Farmacogn., v. 22, n. 4, p. 730-735, 2012.
16. PINTO, E.; CARVALHO, A. P.; CARDOZO, K. H. M.; MALCATA, F. X.; ANJOS, F. M.; COLEPICOLO, P. Effects of heavy metals and light levels on the biosynthesis of carotenoids and fatty acids in the macroalgae Gracilaria tenuistipitata (var. liui Zhang & Xia). Rev. Bras. Farmacogn., v. 21, n. 2, p. 349-354, 2011.
17. RAVEN, P. H.; EVERT, R. F.; EICHHORN, S. E. Biologia vegetal. 7. ed. Rio de Janeiro: Guanabara Koogan, 2007. 830 p.
18. ROSSO, V. V.; MERCADANTE, A. Z. Identification and quantification of carotenoids, by HPLC-PDA-MS/MS, from Amazonian fruits. J. Agric. Food Chem., v. 55, n. 13, p. 5062-5072, 2007.
19. SCHMIDT, É. C.; MARASCHIN, M.; BOUZON, Z. L. Effects of UVB radiation on the carragenophyte Kappaphycus alvarezii (Rhodophyta, Gigartinales): changes in ultrastructure, growth, and photosynthetic pigments. Hydrobiologia, v. 649, n. 1, p. 171-182, 2010.
20. VAN BREEMEN, R. B.; DONG, L.; PAJKOVIC, N. D. Atmospheric pressure chemical ionization tandem mass spectrometry of carotenoids. Int. J. Mass Spectrom., v. 312, p. 163-172, 2011.
21. WELLBURN, A. R. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J. Plant Physiol., v. 144, n. 3, p. 307-313, 1994.
22. WRIGHT, S. W.; JEFFREY, S. W.; MANTOURA, R. F. C. Evaluation of methods and solvents for pigment extraction. In: JEFFREY, S. W.; MANTOURA, R. F. C.; WRIGHT, S. W. (Eds.). Phytoplankton pigments in oceanography: guidelines to modern methods. Paris: UNESCO, 1997. p. 261-282. (Monographs on oceanographic methodology; 10).
23. URSI, S.; PLASTINO, E. M. Crescimento in vitro de linhagens de coloração vermelha e verde clara de Gracilaria birdiae (Gracilariales, Rhodophyta) em dois meios de cultura: análise de diferentes estádios reprodutivos. Rev. Bras. Bot., v. 24, n, 4, supl., p. 587-594, 2001.