Validation of reference genes for normalization gene expression in reverse transcription quantitative PCR in human normal thyroid and goiter tissue

BioMed Research International

Volumn 2014, Issue , 2014, Pages

  Weber, Raquel ^a   Bertoni, Ana Paula Santin ^a,b   Bessestil, Laura Walter ^a   Brasil, Beatriz Maria De Azevedo Assis ^c   Brum, Llma Simoni ^a   Furlanetto, Tania Weber ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b UNIVERSIDADE FEDERAL DE CIÊNCIAS DA SAÚDE DE PORTO ALEGRE   (Brazil)

^c HOSPITAL DE CLÍNICAS DE PORTO ALEGRE   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE 1; PROTEIN; SUCCINATE DEHYDROGENASE; SUCCINATE DEHYDROGENASE SUBUNIT A FLAVOPROTEIN; TRYPTOPHAN 5 MONOOXYGENASE ACTIVATION PROTEIN; TYROSINE 3 MONOOXYGENASE; UNCLASSIFIED DRUG; ZETA POLYPEPTIDE;

ACTB GENE; ARTICLE; B2M GENE; CLINICAL ARTICLE; COMPUTER PROGRAM; CONTROLLED STUDY; GAPDH GENE; GENE; GENE EXPRESSION; GENETIC STABILITY; GOITER; HPRT1 GENE; HUMAN; HUMAN GENOME; HUMAN TISSUE; REFERENCE GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION QUANTITATIVE POLYMERASE CHAIN REACTION; SDHA GENE; THYROID GLAND; VALIDATION STUDY; YWHAZ GENE; GENETICS; METABOLISM; PROCEDURES; REPRODUCIBILITY; STANDARD;

GENE EXPRESSION; GOITER; HUMANS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; THYROID GLAND;

EID: 84901751575     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/198582     Document Type: Article

References (24)

1. Pfaffl M. W., Tichopad A., Prgomet C., Neuvians T. P., Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnology Letters 2004 26 6 509 515 2-s2.0-1842483281 10.1023/B:BILE. 0000019559.84305.47 (Pubitemid 38448421)
2. Bustin S. A., Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. Journal of Molecular Endocrinology 2002 29 1 23 39 2-s2.0-0036668567 10.1677/jme.0.0290023 (Pubitemid 34965744)
3. Bustin S. A., Benes V., Garson J. A., Hellemans J., Huggett J., Kubista M., Mueller R., Nolan T., Pfaffl M. W., Shipley G. L., Vandesompele J., Wittwer C. T., The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 2009 55 4 611 622 2-s2.0-64149097786 10.1373/clinchem.2008.112797
4. Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F., Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 2002 3 7 RESEARCH0034 10.1186/gb-2002-3-7-research0034 2-s2.0-0037129827
5. Thellin O., Zorzi W., Lakaye B., De Borman B., Coumans B., Hennen G., Grisar T., Igout A., Heinen E., Housekeeping genes as internal standards: use and limits. Journal of Biotechnology 1999 75 2-3 291 295 2-s2.0-0032883806 10.1016/S0168-1656(99)00163-7 (Pubitemid 29486627)
6. Radonić A., Thulke S., Mackay I. M., Landt O., Siegert W., Nitsche A., Guideline to reference gene selection for quantitative real-time PCR. Biochemical and Biophysical Research Communications 2004 313 4 856 862 2-s2.0-0346728757 10.1016/j.bbrc.2003.11.177 (Pubitemid 38050071)
7. Haller F., Kulle B., Schwager S., Gunawan B., Heydebreck A. V., Sültmann H., Füzesi L., Equivalence test in quantitative reverse transcription polymerase chain reaction: confirmation of reference genes suitable for normalization. Analytical Biochemistry 2004 335 1 1 9 2-s2.0-7444226814 10.1016/j.ab.2004.08.024 (Pubitemid 39446224)
8. Dundas J., Ling M., Reference genes for measuring mRNA expression. Theory in Biosciences 2012 131 4 215 223
9. Santin A. P., Souza A. F., Brum L. S., Furlanetto T. W., Validation of reference genes for normalizing gene expression in real-time quantitative reverse transcription PCR in human thyroid cells in primary culture treated with progesterone and estradiol. Molecular Biotechnology 2013 54 2 278 282 10.1007/s12033-012-9565-0
10. Chantawibul S., Anuwong A., Leelawat K., Validation of appropriate reference genes for gene expression studies in human thyroid gland using real-time RT-PCR. Journal of the Medical Association of Thailand 2012 95 supplement 3 S36 S40
11. Lecchi C., Dilda F., Sartorelli P., Ceciliani F., Widespread expression of SAA and Hp RNA in bovine tissues after evaluation of suitable reference genes. Veterinary Immunology and Immunopathology 2012 145 1-2 556 562 2-s2.0-84856552710 10.1016/j.vetimm.2011.12.017
12. Li Q., Domig K. J., Ettle T., Windisch W., Mair C., Schedle K., Evaluation of potential reference genes for relative quantification by RT-qPCR in different porcine tissues derived from feeding studies. International Journal of Molecular Sciences 2011 12 3 1727 1734 2-s2.0-79953310768 10.3390/ijms12031727
13. Lisowski P., Pierzchała M., Gościk J., Pareek C. S., Zwierzchowski L., Evaluation of reference genes for studies of gene expression in the bovine liver, kidney, pituitary, and thyroid. Journal of Applied Genetics 2008 49 4 367 372 2-s2.0-56649088074
14. Studer H., Peter H. J., Gerber H., Natural heterogeneity of thyroid cells: the basis for understanding thyroid function and nodular goiter growth. Endocrine Reviews 1989 10 2 125 135 2-s2.0-0024403390 (Pubitemid 19151340)
15. Krohn K., Führer D., Bayer Y., Eszlinger M., Brauer V., Neumann S., Paschke R., Molecular pathogenesis of euthyroid and toxic multinodular goiter. Endocrine Reviews 2005 26 4 504 524 2-s2.0-20044374286 10.1210/er.2004-0005 (Pubitemid 41014264)
16. Baloch Z. W., LiVolsi V. A., Follicular-patterned lesions of the thyroid: the bane of the pathologist. American Journal of Clinical Pathology 2002 117 1 143 150 2-s2.0-0036141552 10.1309/8VL9-ECXY-NVMX-2RQF (Pubitemid 34052484)
17. Souza A. F., Brum I. S., Neto B. S., Berger M., Branchini G., Reference gene for primary culture of prostate cancer cells. Molecular Biology Reports 2012 40 4 2955 2962 10.1007/s11033-012-2366-5
18. Andersen C. L., Jensen J. L., Orntoft T. F., Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research 2004 64 15 5245 5250 2-s2.0-3442876969 10.1158/0008-5472.CAN-04-0496 (Pubitemid 39006544)
19. de Jonge H. J., Fehrmann R. S. N., de Bont E. S. J. M., Hofstra R. M. W., Gerbens F., Kamps W. A., de Vries E. G. E., van der Zee A. G. J., te Meerman G. J., ter Elst A., Evidence based selection of housekeeping genes. PLoS ONE 2007 2 9, article e898 2-s2.0-41149171125 10.1371/journal.pone.0000898 (Pubitemid 351425878)
20. Selvey S., Thompson E. W., Matthaei K., Lea R. A., Irving M. G., Griffiths L. R., β -actin-an unsuitable internal control for RT-PCR. Molecular and Cellular Probes 2001 15 5 307 311 2-s2.0-0035197018 10.1006/mcpr.2001.0376 (Pubitemid 33105000)
21. Ruan W., Lai M., Actin, a reliable marker of internal control? Clinica Chimica Acta 2007 385 1-2 1 5 2-s2.0-34548491164 10.1016/j.cca.2007.07.003 (Pubitemid 47374670)
22. Mori R., Wang Q., Danenberg K. D., Pinski J. K., Danenberg P. V., Both β -actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer. Prostate 2008 68 14 1555 1560 2-s2.0-51649122029 10.1002/pros.20815
23. Mehta R., Birerdinc A., Hossain N., Afendy A., Chandhoke V., Younossi Z., Baranova A., Validation of endogenous reference genes for qRT-PCR analysis of human visceral adipose samples. BMC Molecular Biology 2010 11, article 39 2-s2.0-77952445492 10.1186/1471-2199-11-39
24. Hayashi R., Hayashi S., Arai K., Chikuda M., Obara Y., Effects of antioxidant supplementation on mRNA expression of glucose-6-phosphate dehydrogenase, β -actin and 18S rRNA in the anterior capsule of the lens in cataract patients. Experimental Eye Research 2012 96 1 48 54 2-s2.0-84857801735 10.1016/j.exer.2012.01.001