Hexameric GFP and mCherry reporters for the Drosophila GAL4, Q, and LexA transcription systems

Genetics

Volumn 196, Issue 4, 2014, Pages 951-960

  Shearin, Harold K ^a   MacDonald, Ian S ^a   Spector, Laura P ^a,b   Steven Stowers, R ^a  

^a MONTANA STATE UNIVERSITY   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; LEXA PROTEIN; MCHERRY PROTEIN; PROTEIN; Q PROTEIN; TRANSCRIPTION FACTOR GAL4; UNCLASSIFIED DRUG; DROSOPHILA PROTEIN; GAL4 PROTEIN, DROSOPHILA; HYBRID PROTEIN; PHOTOPROTEIN; TRANSCRIPTION FACTOR;

ADULT; ANIMAL CELL; ARTICLE; BRIGHTNESS; CELL STRUCTURE; CELLULAR DISTRIBUTION; CHEMICAL PHENOMENA; CONTROLLED STUDY; CYTOPLASM; DENDRITE; DROSOPHILA MELANOGASTER; FLUORESCENCE IMAGING; GENETIC SCREENING; IN VIVO STUDY; NERVE FIBER; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SENSORY NERVE CELL; TANDEM FUSION; ANIMAL; GENETICS; METABOLISM; MOLECULAR CLONING; PROCEDURES; REPRODUCIBILITY; TANDEM REPEAT; TRANSGENIC ANIMAL; ULTRASTRUCTURE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; AXONS; CLONING, MOLECULAR; DENDRITES; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; LUMINESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; TANDEM REPEAT SEQUENCES; TRANSCRIPTION FACTORS;

EID: 84901377786     PISSN: 00166731     EISSN: 19432631     Source Type: Journal    
DOI: 10.1534/genetics.113.161141     Document Type: Article

References (31)

1. Brand, A. H., and N. Perrimon, 1993 Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118: 401-415.
2. Bushey, D., G. Tononi, and C. Cirelli, 2009 The Drosophila fragile X mental retardation gene regulates sleep need. J. Neurosci. 29: 1948-1961.
3. Cermak, T., E. L. Doyle, M. Christian, L. Wang, Y. Zhang et al., 2011 Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Res. 39: e82.
4. Chalfie, M, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, 1994 Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
5. Chen, T. W., T. J. Wardill, Y. Sun, S. R. Pulver, S. L. Renninger et al., 2013 Ultrasensitive fluorescent proteins for imaging and neuronal activity. Nature 499: 295-300.
6. Cheo, D. L., S. A. Titus, D. R. Byrd, J. L. Hartley, G. F. Temple et al., 2004 Concerted assembly and cloning of multiple DNA segments using in vitro site-specific recombination: functional analysis of multi-segment expression clones. Genome Res. 14: 2111-2120.
7. Chi, P., P. Greengard, and T. A. Ryan, 2001 Synapsin dispersion and reclustering during synaptic activity. Nat. Neurosci. 4: 1187-1193.
8. Edwards, K. A., M. Demsky, R. A. Montague, N. Weymouth, and D. P. Kiehart, 1997 GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila. Dev. Biol. 191: 103-117.
9. Engler, C., R. Gruetzner, R. Kandzia, and S. Marillonnet, 2009 Golden Gate Shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes. PLoS ONE 4: e5553.
10. Feinberg, E. H., M. K. Vanhoven, A. Bendesky, G. Wang, R. D. Fetter et al., 2008 GFP Reconstitution Across Synaptic Partners (GRASP) defines cell contacts and synapses in living nervous systems. Neuron 57: 353-363.
11. Goedhart, J., D. von Stetten, M. Noirclerc-Savoye, M. Lelimousin, L. Joosen et al., 2012 Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%. Nat. Commun. 3: 751.
12. Groth, A., M. Fish, R. Nusse, and M. P. Calos, 2004 Construction of transgenic Drosophila by using the site-specific integrase from phage PhiC31. Genetics 166: 1775-1782.
13. Halfhill, M. D., R. J. Millwood, A. K. Weissinger, S. I. Warwich, and C. N. Stewart, Jr., 2003 Additive transgene expression and genetic introgression in multiple green-fluorescent protein transgenic crop 3 weed hybrid generations. Theor. Appl. Genet. 107: 1533-1540.
14. Halfon, M. S., S. Gisselbrecht, J. Lu, B. Estrada, H. Keshishian et al., 2002 New fluorescent protein reporters for use with the Drosophila Gal4 expression system and for vital detection of balancer chromosomes. Genesis 34: 135-138.
15. Heim, R., A. B. Cubitt, and R. Y. Tsien, 1995 Improved green fluorescence. Nature 373: 663-664.
16. Hunt, M. E., M. P. Scherrer, F. D. Ferrari, and M. V. Matz, 2010 Very bright green fluorescent proteins from the pontellid copepod Pontella mimocerami. PLoS ONE 5: e11517.
17. Koelle, M. R., W. S. Talbot, W. A. Segraves, M. T. Bender, P. Cherbas et al., 1991 The Drosophila EcR gene encodes an ecdysone receptor, a new member of the steroid receptor superfamily. Cell 67: 59-77.
18. Lee, T., and L. Luo, 1999 Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22: 451-461.
19. Miyawaki, A., J. Llopis, R. Heim, J. M. McCaffery, J. A. Adams et al., 1997 Fluorescent indicators for Ca21 based on green fluorescent proteins and calmodulin. Nature 388: 882-887.
20. Parnas, D., A. P. Haghighi, R. D. Fetter, S.W. Kim, and C. S. Goodman, 2001 Regulation of postsynaptic structure and protein localization by the Rho-type guanine nucleotide exchange factor dPix. Neuron 32: 415-424.
21. Petersen, L. K., and R. S. Stowers, 2011 A Gateway MultiSite recombination cloning toolkit. PLoS ONE 6: e24531.
22. Pfeiffer, B. D., T. T. Ngo, K. L. Hibbard, C. Murphy, A. Jennett et al., 2010 Refinement of tools for targeted gene expression in Drosophila. Genetics 186: 35-55.
23. Pfeiffer, B. D., J. W. Truman, and G. M. Rubin, 2012 Using translational enhancers to increase transgene expression in Drosophila. Proc. Natl. Acad. Sci. USA 109: 6626-6631.
24. Pines, J., 1995 GFP in mammalian cells. Trends Genet. 11: 326-327.
25. Potter, C. J., B. Tasic, E. V. Russler, L. Liang, and L. Luo, 2010 The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell 141: 536-548.
26. Prasher, D. C., V. K. Eckenrode, W. W. Ward, F. G. Prendergast, and M. J. Cormier, 1992 Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111: 229-233.
27. Rizzuto, R., M. Brini, P. Pizzo, M. Murgia, and T. Pozzan, 1995 Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5: 635-642.
28. Shaner, N. C., R. E. Campbell, P. A. Steinbach, B. N. Giepmans, A. E. Palmer et al., 2004 Improved monomeric red, orange, and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22: 1567-1572.
29. Shaner, N. C., G. G. Lambert, A. Chammas, Y. Ni, P. J. Cranfill et al., 2013 A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nat. Methods 10: 407-409.
30. Shearin, H. K., A. R. Dvarshkis, and C. D. Kozeluh, and R. S. Stowers, 2013 Expansion of the Gateway MultiSite recombination cloning toolkit. PLoS ONE 8: e77724.
31. Venken, K. J. T., Y. He, R. A. Hoskins, and H. J. Bellen, 2006 P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science 314: 1747-1751.