Analysis of translation initiation during stress conditions by polysome profiling

Journal of Visualized Experiments

Volumn , Issue 87, 2014, Pages

  Coudert, Laëtitia ^a,b   Adjibade, Pauline ^a,b   Mazroui, Rachid ^a,b  

^a UNIVERSITÉ LAVAL   (Canada)

^b LAVAL UNIVERSITY   (Canada)

Author keywords

Cellular Biology; Issue 87; Polysome profile; Protein and RNA isolation; Stress conditions; Sucrose gradient; Translation initiation

Indexed keywords

EID: 84901228122     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51164     Document Type: Article

References (27)

1. Gebauer, F., & Hentze, M. W. Molecular mechanisms of translational control. Nature Reviews. Molecular Cell Biology. 5 (10), 827-835, doi:10.1038/nrm1488 (2004).
2. Jackson, R. J., Hellen, C. U. T., & Pestova, T. V The mechanism of eukaryotic translation initiation and principles of its regulation. Nature Reviews. Molecular Cell Biology. 11 (2), 113-127, doi:10.1038/nrm2838 (2010).
3. Fournier, M.-J., Gareau, C., & Mazroui, R. The chemotherapeutic agent bortezomib induces the formation of stress granules. Cancer Cell International. 10(12), doi:10.1186/1475-2867-10-12 (2010).
4. Holcik, M., & Sonenberg, N. Translational control in stress and apoptosis. Nature Reviews. Molecular Cell Biology. 6 (4), 318-327, doi:10.1038/nrm1618 (2005).
5. Mazroui, R., Huot, M.-E., Tremblay, S., Filion, C., Labelle, Y., & Khandjian, E. W. Trapping of messenger RNA by Fragile X Mental Retardation protein into cytoplasmic granules induces translation repression. Human Molecular Genetics. 11 (24), 3007-3017 (2002).
6. Mazroui, R., Huot, M.-E., Tremblay, S., Boilard, N., Labelle, Y., & Khandjian, E. W. Fragile X Mental Retardation protein determinants required for its association with polyribosomal mRNPs. Human Molecular Genetics. 12 (23), 3087-3096, doi:10.1093/hmg/ddg335 (2003).
7. Farny, N. G., Kedersha, N. L., & Silver, P. a Metazoan stress granule assembly is mediated by P-eIF2alpha-dependent and-independent mechanisms. RNA (New York, N.Y.). 15 (10), 1814-1821, doi:10.1261/rna.1684009 (2009).
8. Gareau, C., Houssin, E., et al. Characterization of fragile x mental retardation protein recruitment and dynamics in Drosophila stress granules. PLoS ONE. 8(2), doi:10.1371/journal.pone.0055342 (2013).
9. Brackett, D. M., Qing, F., Amieux, P. S., Sellers, D. L., Horner, P. J., & Morris, D. R. FMR1 transcript isoforms: association with polyribosomes; regional and developmental expression in mouse brain. PLoS ONE. 8 (3), doi:10.1371/journal.pone.0058296 (2013).
10. Khandjian, E. W., Huot, M.-E., Tremblay, S., Davidovic, L., Mazroui, R., & Bardoni, B. Biochemical evidence for the association of fragile X mental retardation protein with brain polyribosomal ribonucleoparticles. Proceedings of the National Academy of Sciences of the United States of America. 101(36), 13357-13362, doi:10.1073/pnas.0405398101 (2004).
11. Erikson, A., Winblad, B., & Wallace, W. Translational control of gene expression in the human brain. Prog Neuropsychopharmacol Biol Psychiatry. 13 (3-4), 469-479 (1989).
12. Stephens, S. B., & Nicchitta, C. V In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum. Methods in Enzymology. 431, 47-60, doi:10.1016/S0076-6879(07)31004-5 (2007).
13. Koritzinsky, M., & Wouters, B. G. Hypoxia and regulation of messenger RNA translation. Methods in Enzymology. 435, 247-273, doi:10.1016/S0076-6879(07)35013-1 (2007).
14. Khandjian, E. W., Corbin, F., Woerly, S., & Rousseau, F. The fragile X mental retardation protein is associated with ribosomes. Nature Genetics. 12, 91-93 (1996).
15. Sivan, G., Kedersha, N., & Elroy-Stein, O. Ribosomal slowdown mediates translational arrest during cellular division. Molecular and Cellular Biology. 27, (19), 6639-6646, doi:10.1128/MCB.00798-07 (2007).
16. Thomas, J. D., & Johannes, G. J. Identification of mRNAs that continue to associate with polysomes during hypoxia. RNA. 13, 1116-1131, doi:10.1261/rna.534807.adapt (2007).
17. Kumaraswamy, S., Chinnaiyan, P., Shankavaram, U. T., Lü, X., Camphausen, K., & Tofilon, P. J. Radiation-induced gene translation profiles reveal tumor type and cancer-specific components. Cancer Research. 68, (10), 3819-3826, doi:10.1158/0008-5472.CAN-08-0016 (2008).
18. Fournier, M.-J., Coudert, L., et al. Inactivation of the mTORC1-eIF4E Pathway alters Stress Granules Formation. Molecular and Cellular Biology. 33, (11), 2285-2301, doi:10.1128/MCB.01517-12 (2013).
19. Sanchez, G., Dury, A. Y., et al. A novel function for the survival motoneuron protein as a translational regulator. Human Molecular Genetics. 22, (4), 668-684, doi:10.1093/hmg/dds474 (2013).
20. Béchade, C., Rostaing, P., et al. Subcellular distribution of survival motor neuron (SMN) protein: possible involvement in nucleocytoplasmic and dendritic transport. The European Journal of Neuroscience. 11, (1), 293-304 (1999).
21. Goulet, I., Boisvenue, S., Mokas, S., Mazroui, R., & Côté, J. TDRD3, a novel Tudor domain-containing protein, localizes to cytoplasmic stress granules. Human Molecular Genetics. 17, (19), 3055-3074, doi:10.1093/hmg/ddn203 (2008).
22. Nottrott, S., Simard, M. J., & Richter, J. D. Human let-7a miRNA blocks protein production on actively translating polyribosomes. Nature Structural & Molecular Biology. 13, (12), 1108-1114, doi:10.1038/nsmb1173 (2006).
23. Genolet, R., Araud, T., Maillard, L., Jaquier-Gubler, P., & Curran, J. An approach to analyse the specific impact of rapamycin on mRNA-ribosome association. BMC Medical Genomics. 1, (33), doi:10.1186/1755-8794-1-33 (2008).
24. Del Prete, M. J., Vernal, R., Dolznig, H., Müllner, E. W., & Garcia-Sanz, J. a Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments. RNA (New York, N.Y.). 13 (3), 414-421, doi:10.1261/rna.79407 (2007).
25. Thoreen, C. C., Chantranupong, L., Keys, H. R., Wang, T., Gray, N. S., & Sabatini, D. M. A unifying model for mTORC1-mediated regulation of mRNA translation. Nature. 485 (7396), 109-113, doi:10.1038/nature11083 (2012).
26. Ingolia, N. T., Brar, G. A., Rouskin, S., Mcgeachy, A. M., & Weissman, J. S. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nature Protocols. 7 (8), 1534-1550, doi:10.1038/nprot.2012.086.The (2012).
27. Morris, D. R. Ribosomal footprints on a transcriptome landscape. Genome Biology. 10 (4), 215, doi:10.1186/gb-2009-10-4-215 (2009).