Expression and purification of soluble recombinant full length HIV-1 Pr55Gag protein in Escherichia coli

Protein Expression and Purification

Volumn 100, Issue , 2014, Pages 10-18

  McKinstry, William J ^a   Hijnen, Marcel ^b,c   Tanwar, Hanumant S ^a,d   Sparrow, Lindsay G ^a   Nagarajan, Sureshbabu ^a,d   Pham, Son T ^a,d   Mak, Johnson ^a,b,c,d  

^a CSIRO   (Australia)

^b BURNET INSTITUTE   (Australia)

^c MONASH UNIVERSITY   (Australia)

^d DEAKIN UNIVERSITY   (Australia)

Author keywords

Gag; HIV 1; Purification; Recombinant protein expression

Indexed keywords

METAL; P55 GAG PRECURSOR PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; PROTEIN PRECURSOR; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; BACTERIUM TRANSFORMATION; CHEMISTRY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROCEDURES; SOLUBILITY; ULTRASTRUCTURE; VIROLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENE EXPRESSION; HIV INFECTIONS; HIV-1; HUMANS; METALS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN PRECURSORS; RECOMBINANT PROTEINS; SOLUBILITY; TRANSFORMATION, BACTERIAL;

EID: 84901035187     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2014.04.013     Document Type: Article

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