Preparation of recombinant HIV-1 Gag protein and assembly of virus-like particles in vitro

Methods in Molecular Biology

Volumn 485, Issue , 2009, Pages 197-208

  Datta, Siddhartha A K ^a   Rein, Alan ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Electron microscopy; Gag; HIV 1; Recombinant protein purification; Virus assembly; Virus like particle

Indexed keywords

GAG PROTEIN; RECOMBINANT PROTEIN; VIROSOME;

ARTICLE; CELL FREE SYSTEM; GENETICS; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; VIRUS ASSEMBLY;

CELL-FREE SYSTEM; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; HIV-1; HUMANS; RECOMBINANT PROTEINS; VIROSOMES; VIRUS ASSEMBLY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 60349108481     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-170-3_14     Document Type: Article

References (4)

1. Campbell S., Vogt, V. M. (1995) Self-assembly in vitro of purified CA-NC proteins from Rous sarcoma virus and human immunodeficiency virus type 1. J Virol 69, 6487-6497.
2. Gross, I., Hohenberg, H., Wilk, T., et al. (2000) A conformational switch controlling HIV-1 morphogenesis. EMBO J 19, 103-113.
3. Campbell, S., Vogt, V. M. (1997) In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles. J Virol 71, 4425-4435.
4. Peterson, E. A., Sober, H. (1962) Column chromatography of proteins: substituted celluloses. Methods Enzymol 5, 3-27.