Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications

Journal of Laboratory Automation

Volumn 19, Issue 3, 2014, Pages 285-296

  Glynn, Macdara ^a   Kirby, Daniel ^a   Chung, Danielle ^a   Kinahan, David J ^a   Kijanka, Gregor ^a   Ducrée, Jens ^a  

^a DUBLIN CITY UNIVERSITY   (Ireland)

Author keywords

cell purification; cell separation; centrifugal microfluidics; HIV AIDS diagnostics; lab on a disc

Indexed keywords

BLOOD; CELLS; DISEASES; MICROFLUIDICS; PURIFICATION; SEPARATION;

CELL PURIFICATION; CELL SEPARATION; CENTRIFUGAL MICROFLUIDICS; HIV/AIDS; LAB-ON-A-DISC;

CYTOLOGY;

ACQUIRED IMMUNE DEFICIENCY SYNDROME; ANTIGEN PURIFICATION; ANTIGEN SPECIFICITY; ARTICLE; AUTOMATION; CD4 LYMPHOCYTE COUNT; CELL CULTURE; CELL ISOLATION; DISC FABRICATION; ELECTRONICS; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS; IMAGING; MAGNETIC FIELD; MICROFLUIDICS; PRIMING; BLOOD BUFFY COAT; CD4+ T LYMPHOCYTE; CENTRIFUGATION; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; DEVICES; ECONOMICS; HEALTH CARE COST; HELA CELL LINE; HL-60 CELL LINE; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOLOGY; IMMUNOMAGNETIC SEPARATION; LAB ON A CHIP; LABORATORY AUTOMATION; METABOLISM; PATHOLOGY; POINT OF CARE SYSTEM; POINT OF CARE TESTING; PROOF OF CONCEPT; SPECTROFLUOROMETRY; VALIDATION STUDY;

FLUORESCENT DYE; MICROSPHERE;

ACQUIRED IMMUNODEFICIENCY SYNDROME; AUTOMATION, LABORATORY; BLOOD BUFFY COAT; CD4-POSITIVE T-LYMPHOCYTES; CENTRIFUGATION; FLUORESCENT DYES; HEALTH CARE COSTS; HELA CELLS; HIV INFECTIONS; HL-60 CELLS; HUMANS; IMMUNOMAGNETIC SEPARATION; LAB-ON-A-CHIP DEVICES; MICROFLUIDICS; MICROSPHERES; POINT-OF-CARE SYSTEMS; POINT-OF-CARE TESTING; PROOF OF CONCEPT STUDY; SPECTROMETRY, FLUORESCENCE;

EID: 84901033091     PISSN: 22110682     EISSN: 22110690     Source Type: Journal    
DOI: 10.1177/2211068213504759     Document Type: Article

References (35)

1. Rapid Advice: Antiretroviral Therapy for HIV Infection in Adults and Adolescents. Geneva, Switzerland: World Health Organization; 2009:.
2. Cheson B. D.,Bennett J. M.,Grever M., et al.National Cancer Institute-Sponsored Working Group Guidelines for Chronic Lymphocytic Leukemia: Revised Guidelines for Diagnosis and Treatment.Blood. 1996;87:4990-4997.
3. Pavlos M.,Michael K.Diagnostic Value of Circulating Tumor Cell Detection in Bladder and Urothelial Cancer: Systematic Review and Meta-analysis.BMC Cancer. 2011;11:336-350.
4. Anderson D.,Crowe S.,Garcia M.Point-of-Care Testing.Curr. HIV/AIDS Rep. 2011;8:31-37.
5. Chin C. D.,Linder V.,Sia S. K.Commercialization of Microfluidic Point-of-Care Diagnostic Devices.Lab Chip. 2012;12:2118-2134.
6. Murtagh M.UNITAIDS Technical Report: HIV/AIDS Diagnostic Landscape.HIV/AIDS Diagnostic Landscape. 2011;:.
7. Jani I. V.,Sitoe N. E.,Alfai E. R., et al.Effect of Point-of-Care CD4 Cell Count Tests on Retention of Patients and Rates of Antiretroviral Therapy Initiation in Primary Health Clinics: An Observational Cohort Study.Lancet. 2011;378:15721579.
8. Glynn M. T.,Kinahan D.,Ducree J.CD4 Counting Technologies for HIV Therapy Monitoring in Resource-Poor Settings: State-of-the-Art and Emerging Microtechnologies.Lab Chip. 2013;13:2731-2748.
9. Boyle D. S.,Hawkins K. R.,Steele M. S., et al.Emerging Technologies for Point-of-Care CD4 T-Lymphocyte Counting.Trends Biotechnol. 2012;30:45-54.
10. Furdui V. I.,Harrison D. J.Immunomagnetic T Cell Capture from Blood for PCR Analysis Using Microfluidic Systems.Lab Chip. 2004;4:614-618.
11. Gao D.,Li H.,Guo G., et al.Magnetic Bead Based Immunoassay for Enumeration of CD4+ T Lymphocytes on a Microfluidic Device.Talanta. 2010;82:528-533.
12. Ymeti A.,Li X.,Lunter B., et al.A Single Platform Image Cytometer for Resource-Poor Settings to Monitor Disease Progression in HIV Infection.Cytometry Part A 71A. 2007;:132-142.
13. Li X.,Breukers C.,Ymeti A., et al.CD4 and CD8 Enumeration for HIV Monitoring in Resource-Constrained Settings 2009.Cytometry B Clin. Cytom 76B:118-126.
14. Burger R.,Ducrée J.Handling and Analysis of Cells and Bioparticles on Centrifugal Microfluidic Platforms.Expert Rev. Mol. Diagn. 2012;12:407-421.
15. Burger R.,Kirby D.,Glynn M., et al.Centrifugal Microfluidics for Cell Analysis.Curr. Opin. Chem. Biol. 2012;16:409-414.
16. Ducrée J.,Haeberle S.,Lutz S., et al.The Centrifugal Microfluidic Bio-Disk Platform.J. Micromech. Microeng. 2007;17:S103-S115.
17. Kirby D.,Siegrist J.,Kijanka G., et al.Centrifugo-magnetophoretic Particle Separation.Microfluid. Nanofluid. 2012;16:1-10.
18. Boettcher M.,Jaeger M. S.,Riegger L., et al. Paper presented at the conference on Bio-Systems; 443.
19. Pamme N.,Wilhelm C.Continuous Sorting of Magnetic Cells via On-Chip Free-Flow Magnetophoresis.Lab Chip. 2006;6:974-980.
20. Mach A. J.,Kim J. H.,Arshi A., et al.Automated Cellular Sample Preparation Using a Centrifuge-on-a-Chip.Lab Chip. 2011;11:2827-2834.
21. Panaro N. J.,Lou X. J.,Fortina P., et al.Micropillar Array Chip for Integrated White Blood Cell Isolation and PCR.Biomol. Eng. 2005;21:157-162.
22. Steigert J.,Haeberle S.,Brenner T., et al.Rapid Prototyping of Microfluidic Chips in COC.J. Micromech. Microeng. 2007;17:333-341.
23. Hosokawa K.,Sato K.,Ichikawa N., et al.Power-free Poly(dimethylsiloxane) Microfluidic Devices for Gold Nanoparticle-Based DNA Analysis.Lab Chip. 2004;4:181-185.
24. Meeker D.Finite Element Method Magnets.Finite Element Method Magnetics. 4:2.
25. Rasband W.ImageJ. Bethesda, MA: National Institutes of Health; 1997-2012.
26. Watkins N. N.,Sridhar S.,Cheng X., et al.A Microfabricated Electrical Differential Counter for the Selective Enumeration of CD4+ T Lymphocytes.Lab Chip. 2011;11:1437-1447.
27. Burger R.,Reith P.,Kijanka G., et al.Array-Based Capture, Distribution, Counting and Multiplexed Assaying of Beads on a Centrifugal Microfluidic Platform.Lab Chip. 2012;12:1289-1295.
28. Steigert J.,Brenner T.,Grumann M., et al.Integrated Siphon-Based Metering and Sedimentation of Whole Blood on a Hydrophilic Lab-on-a-Disk.Biomed. Microdevices. 2007;9:675-679.
29. Riegger L.,Grumann M.,Steigert J., et al.Single-Step Centrifugal Hematocrit Determination on a 10-$ Processing Device.Biomed. Microdevices. 2007;9:795-799.
30. Haeberle S.,Brenner T.,Zengerle R., et al.Centrifugal Extraction of Plasma from Whole Blood on a Rotating Disk.Lab Chip. 2006;6:776-781.
31. Jensen B. N.,Lisse I. M.,Gerstoft J., et al.Cellular Profiles in Bronchoalveolar Lavage Fluid of HIV-Infected Patients with Pulmonary Symptoms: Relation to Diagnosis and Prognosis.AIDS. 1991;5:527-534.
32. Geneva, Switzerland: World Health Organization; 2010:.
33. Haferlach T.,Kohlmann A.,Schnittger S., et al.Global Approach to the Diagnosis of Leukemia Using Gene Expression Profiling.Blood. 2005;106:1189-1198.
34. Deman J. J.,Bruyneel E. A.,Mareel M. M.A Study on the Mechanism of Intercellular Adhesion.J. Cell Biol. 1974;60:641-652.
35. Grundy C. L.,Medina Lara A.,Winogron D., et al.Point-of-Care CD4 Tests Can Increase Life-Years Saved with Reduced Costs Compared to Flow Cytometric CD4 Counting. Sixth International AIDS Society Conference on HIV Pathogenesis, Treatment and Prevention.