MAVIS: An integrated system for live microscopy and vitrification

Ultramicroscopy

Volumn 143, Issue , 2014, Pages 67-76

  Koning, Roman I ^a   Faas, Frank G ^a   Boonekamp, Michael ^a   de Visser, Bram ^a   Janse, Jan ^a   Wiegant, Joop C ^a   de Breij, Anna ^a   Willemse, Joost ^a   Nibbering, Peter H ^a   Tanke, Hans J ^a   Koster, Abraham J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Correlative microscopy; Cryo microscopy; Electron microscopy; Light microscopy

Indexed keywords

BACTERIA; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; INTEGRATED CONTROL; OPTICAL MICROSCOPY; VITRIFICATION;

BIOLOGICAL SAMPLES; CORRELATIVE MICROSCOPIES; CRYO-ELECTRON MICROSCOPY; CRYO-MICROSCOPY; INTEGRATED SYSTEMS; MICROSCOPIC IMAGING; NANOMETER-SCALE RESOLUTION; THREE-DIMENSIONAL RECONSTRUCTION;

ELECTRON MICROSCOPES;

ACINETOBACTER BAUMANNII; ARTICLE; BRIGHT FIELD MICROSCOPY; CRYOELECTRON MICROSCOPY; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; MICROSCOPE; MICROSCOPY; MICROSCOPY AND VITRIFICATION INTEGRATED SYSTEM; NONHUMAN; U2OS CELL LINE; VITRIFICATION; CELL LINE; CRYOPRESERVATION; HUMAN; MACROMOLECULE; METABOLISM; POLARIZATION MICROSCOPY; PROCEDURES;

MACROMOLECULE;

CELL LINE; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; HUMANS; MACROMOLECULAR SUBSTANCES; MICROSCOPY, FLUORESCENCE; MICROSCOPY, POLARIZATION; VITRIFICATION;

EID: 84900853816     PISSN: 03043991     EISSN: 18792723     Source Type: Journal    
DOI: 10.1016/j.ultramic.2013.10.007     Document Type: Article

References (25)

1. Wang Q., Mercogliano C.P., Lowe J. A ferritin-based label for cellular electron cryotomography. Structure 2011, 19:147-154.
2. T. Mueller-Reichert, P. Verkade (Eds.), Correlative Light and Electron Microscopy, 2012, Academic Press.
3. Schwartz C.L., Sarbash V.I., Ataullakhanov F.I., McIntosh J.R., Nicastro D. Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J. Microsc. 2007, 227:98-109.
4. Koning R.I., Zovko S., Barcena M., Oostergetel G.T., Koerten H.K., Galjart N., Koster A.J., Mieke M.A. Cryo electron tomography of vitrified fibroblasts: microtubule plus ends in situ. J. Struct. Biol. 2008, 161:459-468.
5. Patla I., Volberg T., Elad N., Hirschfeld-Warneken V., Grashoff C., Fassler R., Spatz J.P., Geiger B., Medalia O. Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat. Cell Biol. 2010, 12:909-915.
6. Mader A., Elad N., Medalia O. Cryoelectron tomography of eukaryotic cells. Methods Enzymol. 2010, 483:245-265.
7. Pilhofer M., Ladinsky M.S., McDowall A.W., Jensen G.J. Bacterial TEM: new insights from cryo-microscopy. Methods Cell Biol. 2010, 96:21-45.
8. Spiegelhalter C., Tosch V., Hentsch D., Koch M., Kessler P., Schwab Y., Laporte J. From dynamic live cell imaging to 3D ultrastructure: novel integrated methods for high pressure freezing and correlative light-electron microscopy. PLoS One 2010, 5:e9014.
9. Verkade P. Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J. Microsc. 2008, 230:317-328.
10. Jun S., Ke D., Debiec K., Zhao G., Meng X., Ambrose Z., Gibson G.A., Watkins S.C., Zhang P. Direct visualization of HIV-1 with correlative live-cell microscopy and cryo-electron tomography. Structure 2011, 19:1573-1581.
11. Faas F.G., Barcena M., Agronskaia A.V., Gerritsen H.C., Moscicka K.B., Diebolder C.A., van Driel L.F., Limpens R.W., Bos E., Ravelli R.B., Koning R.I., Koster A.J. Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron microscopy. J. Struct. Biol. 2013, 181:283-290.
12. Agronskaia A.V., Valentijn J.A., van Driel L.F., Schneijdenberg C.T., Humbel B.M., van Bergen en Henegouwen P.M., Verkleij A.J., Koster A.J., Gerritsen H.C. Integrated fluorescence and transmission electron microscopy. J. Struct. Biol. 2008, 164:183-189.
13. Tivol W.F., Briegel A., Jensen G.J. An improved cryogen for plunge freezing. Microsc. Microanal. 2008, 14:375-379.
14. Brouwer A.K., Schimmel J., Wiegant J.C., Vertegaal A.C., Tanke H.J., Dirks R.W. Telomeric DNA mediates de novo PML body formation. Mol. Biol. Cell 2009, 20:4804-4815.
15. van Driel L.F., Valentijn J.A., Valentijn K.M., Koning R.I., Koster A.J. Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells. Eur. J. Cell Biol. 2009, 88:669-684.
16. W.S. Rasband, ImageJ, in: http://imagej.nih.gov/ij/, U. S. National Institutes of Health, Bethesda, Maryland, USA, 1997-2012.
17. Polishchuk R.S., Polishchuk E.V., Luini A. Visualizing live dynamics and ultrastructure of intracellular organelles with preembedding correlative light-electron microscopy. Methods Cell Biol. 2012, 111:21-35.
18. van Rijnsoever C., Oorschot V., Klumperman J. Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nat. Methods 2008, 5:973-980.
19. Brown E., Mantell J., Carter D., Tilly G., Verkade P. Studying intracellular transport using high-pressure freezing and correlative light electron microscopy. Semin. Cell Dev. Biol. 2009, 20:910-919.
20. Perrot R., Julien J.P. Real-time imaging reveals defects of fast axonal transport induced by disorganization of intermediate filaments. FASEB J. 2009, 23:3213-3225.
21. Stepanova T., Slemmer J., Hoogenraad C.C., Lansbergen G., Dortland B., De Zeeuw C.I., Grosveld F., van Cappellen G., Akhmanova A., Galjart N. Visualization of microtubule growth in cultured neurons via the use of EB3-GFP (end-binding protein 3-green fluorescent protein). J. Neurosci. 2003, 23:2655-2664.
22. Carlson D.B., Gelb J., Palshin V., Evans J.E. Laboratory-based cryogenic soft x-ray tomography with correlative cryo-light and electron microscopy. Microsc. Microanal. 2013, 19:22-29.
23. McDermott G., Le Gros M.A., Larabell C.A. Visualizing cell architecture and molecular location using soft x-ray tomography and correlated cryo-light microscopy. Annu. Rev. Phys. Chem. 2012, 63:225-239.
24. Liv N., Zonnevylle A.C., Narvaez A.C., Effting A.P., Voorneveld P.W., Lucas M.S., Hardwick J.C., Wepf R.A., Kruit P., Hoogenboom J.P. Simultaneous correlative scanning electron and high-NA fluorescence microscopy. PLoS One 2013, 8:e55707.
25. Zonnevylle A.C., RF V.A.N.T., Liv N., Narvaez A.C., Effting A.P., Kruit P., Hoogenboom J.P. Integration of a high-NA light microscope in a scanning electron microscope. J. Microsc. 2013, 252:58-70.