Cryo electron tomography of vitrified fibroblasts: Microtubule plus ends in situ

Journal of Structural Biology

Volumn 161, Issue 3, 2008, Pages 459-468

  Koning, Roman I ^a   Zovko, Sandra ^a   Bárcena, Montserrat ^a   Oostergetel, Gert T ^b   Koerten, Henk K ^a   Galjart, Niels ^c   Koster, Abraham J ^a   Mieke Mommaas, A ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Cryo electron microscopy; Cryo electron tomography; Cytoskeleton; Fibroblasts; Luminal particles; Microtubule plus end; Microtubules; Protofilaments; Vitrification

Indexed keywords

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRYOELECTRON TOMOGRAPHY; ELECTRON BEAM TOMOGRAPHY; EMBRYO; FIBROBLAST; MICROTUBULE; MOUSE; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE;

ANIMALS; CRYOELECTRON MICROSCOPY; CRYOPRESERVATION; FIBROBLASTS; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MICROSCOPY, FLUORESCENCE; MICROTUBULES; TOMOGRAPHY;

LEIS;

EID: 40649096358     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2007.08.011     Document Type: Article

References (35)

1. Akhmanova A., and Hoogenraad C.C. Microtubule plus-end-tracking proteins: mechanisms and functions. Curr. Opin. Cell Biol. 17 (2005) 47-54
2. Akhmanova A., Hoogenraad C.C., Drabek K., Stepanova T., Dortland B., Verkerk T., Vermeulen W., Burgering B.M., De Zeeuw C.I., Grosveld F., and Galjart N. Clasps are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts. Cell 104 (2001) 923-935
3. Al Amoudi A., Norlean L.P.O., and Dubochet J. Cryo-electron microscopy of vitreous sections of native biological cells and tissues. J. Struct. Biol. 149 (2005) 225 (vol. 148, p. 131, 2004)
4. Austin J.R., Segui-Simarro J.M., and Staehelin L.A. Quantitative analysis of changes in spatial distribution and plus-end geometry of microtubules involved in plant-cell cytokinesis. J. Cell Sci. 118 (2005) 3895-3903
5. Bouchet-Marquis C., Zuber B., Glynn A.M., Eltsov M., Grabenbauer M., Goldie K.N., Thomas D., Frangakis A.S., Dubochet J., and Chretien D. Visualization of cell microtubules in their native state. Biol. Cell 99 (2007) 45-53
6. Comolli L.R., Kundmann M., and Downing K.H. Characterization of intact subcellular bodies in whole bacteria by cryo-electron tomography and spectroscopic imaging. J. Microsc. 223 (2006) 40-52
7. Crowther R.A., De Rosier D.J., and Klug A. The reconstruction of a three-dimensional structure from projections and its application to electron microscopy. Proc. Roy. Soc. Lond. 317 (1970) 319-340
8. Dubochet J., Adrian M., Chang J.J., Homo J.C., Lepault J., Mcdowall A.W., and Schultz P. Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21 (1988) 129-228
9. Frangakis A.S., Bohm J., Forster F., Nickell S., Nicastro D., Typke D., Hegerl R., and Baumeister W. Identification of macromolecular complexes in cryoelectron tomograms of phantom cells. Proc. Natl. Acad. Sci. USA 99 (2002) 14153-14158
10. Frangakis A.S., and Hegerl R. Noise reduction in electron tomographic reconstructions using nonlinear anisotropic diffusion. J. Struct. Biol. 135 (2001) 239-250
11. Garvalov B.K., Zuber B., Bouchet-Marquis C., Kudryashev M., Gruska M., Beck M., Leis A., Frischknecht F., Bradke F., Baumeister W., Dubochet J., and Cyrklaff M. Luminal particles within cellular microtubules. J. Cell Biol. 174 (2006) 759-765
12. Grimm R., Singh H., Rachel R., Typke D., Zillig W., and Baumeister W. Electron tomography of ice-embedded prokaryotic cells. Biophys. J. 74 (1998) 1031-1042
13. Grimm R., Typke D., Barmann M., and Baumeister W. Determination of the inelastic mean free path in ice by examination of tilted vesicles and automated most probable loss imaging. Ultramicroscopy 63 (1996) 169-179
14. Hoog J.L., Schwartz C., Noon A.T., O′toole E.T., Mastronarde D.N., McIntosh J.R., and Antony C. Organization of interphase microtubules in fission yeast analyzed by electron tomography. Dev. Cell 12 (2007) 349-361
15. Hsieh C.E., Leith A., Mannella C.A., Frank J., and Marko M. Towards high-resolution three-dimensional imaging of native mammalian tissue: electron tomography of frozen-hydrated rat liver sections. J. Struct. Biol. 153 (2006) 1-13
16. Komarova Y.A., Akhmanova A.S., Kojima S., Galjart N., and Borisy G.G. Cytoplasmic linker proteins promote microtubule rescue in vivo. J. Cell Biol. 159 (2002) 589-599
17. Koster A.J., and Barcena M. Electron tomography: methods for three-dimensional visualization of structures in the cell. In: Frank J. (Ed). Electron Tomography: Methods for Three-Dimensional Visualization of Structures in the Cell (2007), Springer, NY 113-163
18. Koster A.J., Grimm R., Typke D., Hegerl R., Stoschek A., Walz J., and Baumeister W. Perspectives of molecular and cellular electron tomography. J. Struct. Biol. 120 (1997) 276-308
19. Kremer J.R., Mastronarde D.N., and McIntosh J.R. Computer visualization of three-dimensional image data using IMOD. J. Struct. Biol. 116 (1996) 71-76
20. Mandelkow E.M., Mandelkow E., and Milligan R.A. Microtubule dynamics and microtubule caps: a time-resolved cryo-electron microscopy study. J. Cell Biol. 114 (1991) 977-991
21. Medalia O., Beck M., Ecke M., Weber I., Neujahr R., Baumeister W., and Gerisch G. Organization of actin networks in intact filopodia. Curr. Biol. 17 (2007) 79-84
22. Medalia O., Weber I., Frangakis A.S., Nicastro D., Gerisch G., and Baumeister W. Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography. Science 298 (2002) 1209-1213
23. Murphy G.E., Leadbetter J.R., and Jensen G.J. In situ structure of the complete Treponema primitia flagellar motor. Nature 442 (2006) 1062-1064
24. Nicastro D., Frangakis A.S., Typke D., and Baumeister W. Cryo-electron tomography of neurospora mitochondria. J. Struct. Biol. 129 (2000) 48-56
25. Nicastro D., Schwartz C., Pierson J., Gaudette R., Porter M.E., and McIntosh J.R. The molecular architecture of axonemes revealed by cryoelectron tomography. Science 313 (2006) 944-948
26. Nickell S., Hegerl R., Baumeister W., and Rachel R. Pyrodictium cannulae enter the periplasmic space but do not enter the cytoplasm, as revealed by cryo-electron tomography. J. Struct. Biol. 141 (2003) 34-42
27. O′toole E.T., McDonald K.L., Mantler J., McIntosh J.R., Hyman A.A., and Muller-Reichert T. Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans. J. Cell Biol. 163 (2003) 451-456
28. O′toole E.T., McDonald K.L., Mantler J., McIntosh J.R., Hyman A.A., and Muller-Reichert T. Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans. J. Cell Biol. 163 (2003) 451-456
29. Ortiz J.O., Forster V., Kurner J., Linaroudis A.A., and Baumeister W. Mapping 70S ribosomes in intact cells by cryoelectron tomography and pattern recognition. J. Struct. Biol. 156 (2006) 334-341
30. Peris L., Thery M., Faure J., Saoudi Y., Lafanechere L., Chilton J.K., Gordon-Weeks P., Galjart N., Bornens M., Wordeman L., Wehland J., Andrieux A., and Job D. Tubulin tyrosination is a major factor affecting the recruitment of CAP-Gly proteins at microtubule plus ends. J. Cell Biol. 174 (2006) 839-849
31. Plitzko J., and Baumeister W. Cryoelectron tomography. In: Hawkes P.W., and Spence J.C.H. (Eds). Science of Microscopy (2006), Springer, NY 529-598
32. Seybert A., Herrmann R., and Frangakis A.S. Structural analysis of Mycoplasma pneumoniae by cryo-electron tomography. J. Struct. Biol. 156 (2006) 342-354
33. Sui H., and Downing K.H. Molecular architecture of axonemal microtubule doublets revealed by cryo-electron tomography. Nature 442 (2006) 475-478
34. Vandenbeldt K.J., Barnard R.M., Hergert P.J., Meng X., Maiato H., and McEwen B.F. Kinetochores use a novel mechanism for coordinating the dynamics of individual microtubules. Curr. Biol. 16 (2006) 1217-1223
35. Vidali L., Chen F., Cicchetti G., Ohta Y., and Kwiatkowski D.J. Rac1-null mouse embryonic fibroblasts are motile and respond to platelet-derived growth factor. Mol. Biol. Cell 17 (2006) 2377-2390