Modification of seed oil composition in Arabidopsis by artificial microRNA-mediated gene silencing

Frontiers in Plant Science

Volumn 3, Issue JUL, 2012, Pages

  Belide, Srinivas ^a,b   Petrie, James Robertson ^a   Shrestha, Pushkar ^a   Singh, Surinder Pal ^a  

^a CSIRO   (Australia)

^b SREENIDHI INSTITUTE OF SCIENCE AND TECHNOLOGY   (India)

Author keywords

Fatty acid desaturase 2; Fatty acid elongase; Fatty acid profiles; Fatty acyl ACP thioesterase B; miR159b

Indexed keywords

EID: 84900812175     PISSN: None     EISSN: 1664462X     Source Type: Journal    
DOI: 10.3389/fpls.2012.00168     Document Type: Article

References (37)

1. Ai, T., Zhang, L., Gao, Z., Zhu, C. X., and Guo, X. (2011). Highly efficient virus resistance mediated by artificial microRNAs that target the suppressor of PVX and PVY in plants. Plant Biol. 13, 304-316.
2. Alvarez, J. P., Pekker, I., Goldshmidt, A., Blum, E., Amsellem, Z., and Eshed, Y. (2006). Endogenous and synthetic microRNAs stimulate simultaneous, efficient, and localized regulation of multiple targets in diverse species. Plant Cell 18, 1134-1151.
3. Clough, S. J., and Bent, A. F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation ofArabidopsis thaliana. Plant J. 16, 735-743.
4. Coutu, C., Brandle, J., Brown, D., Brown, K., Miki, B., Simmonds, J., and Hegedus, D. (2007). pORE: a modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res. 16, 771-781.
5. Duan, C. G., Wang, C. H., Fang, R. X., and Guo, H. S. (2008). Artificial microRNAs highly accessible to targets confer efficient virus resistance in plants. J. Virol. 82, 11084-11095.
6. Eamens, A. L., Smith, N. A., Curtin, S. J., Wang, M., and Waterhouse, P. M. (2009). The Arabidopsis thalianadouble-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes. RNA 15, 2219-2235.
7. Gaurav, S., Álvaro Pérez-Quintero, L., Mehedi, H., Tatiana, V. T., and López, C. (2011). Artificial microRNAs (amiRNAs) engineering-On how microRNA-based silencing methods have affected current plant silencing research. Biochem. Biophys. Res. Commun. 406, 315-319.
8. Gustavo, B., Joaquin, J. S., Michael, R. P., and John, B. O. (2003). Disruption of the FATB gene in Arabidopsisdemonstrates an essential role of saturated fatty acids in plant growth. Plant Cell 15, 1020-1033.
9. Haney, C. H., and Long, S. R. (2010). Plant flotillins are required for infection by nitrogen fixing bacteria. Proc. Natl. Acad. Sci. U.S.A. 107, 478-483.
10. Herve, V., Vazquez, F., and Crété, P. (2004). The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development. Genes Dev. 18, 1187-1197.
11. Horn, M., and Waterhouse, P. (2010). Rapid match-searching for gene silencing assessment. Bioinformatics 26, 1932-1937.
12. Jadhav, A., Katavic, V., Marillia, E. F., Michael Giblin, E., Barton, D. L., Kumar, A., Sonntag, C., Babic, V., Keller, W. A., and Taylor, D. C. (2005). Increased levels of erucic acid in Brassica carinata by co-suppression and antisense repression of the endogenous FAD2 gene. Metab. Eng. 7, 215-220.
13. Kanrar, S., Venkateswari, J., Dureja, P., Kirti, P. B., and Chopra, V. L. (2006). Modification of erucic acid content in Indian mustard (Brassica juncea) by up-regulation and down-regulation of the Brassica juncea fatty acid elongation1 (BjFAE1) gene. Plant Cell Rep. 25, 148-155.
14. Khraiwesh, B., Ossowski, S., Weigel, D., and Reski, W. (2008). pdf Frank specific gene silencing by artificial microRNAs in Physcomitrella patens: an alternative to targeted gene knockouts. Plant Physiol. 148, 684-693.
15. Liu, Q., Surinder, S., and Green, A. (2002). High-stearic and high-oleic cottonseed oils produced by hpRNA-mediated posttranscriptional gene silencing. Plant Physiol. 129, 1732-1743.
16. Maria, G.-N., Aukerman, M. J., Hajime, S., Scott, V. T., and Robert, W. W. (2008). Artificial trans-acting siRNAs confer consistent and effective gene silencing. Plant Physiol. 147, 543-551.
17. Miquel, M., and Browse, J. (1992). Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase. J. Biol. Chem. 267, 1502-1509.
18. Molnar, A., Bassett, A., Thuenemann, E., Schwach, F., Karkare, S., Ossowski, S., Weigel, D., and Baulcombe, D. (2009). Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii. Plant J. 58, 165-174.
19. Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.
20. Napier, J. A. (2007). The production of unusual fatty acids in transgenic plants. Annu. Rev. Plant Biol. 58, 95-319.
21. Niu, Q. W., Lin, S. S., Reyes, J. L., Chen, K. C., Wu, H. W., Yeh, S. D., and Nam-Hai, C. (2006). Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat. Biotechnol. 24, 1420-1428.
22. Okuley, J., Lightner, J., Feldmann, K., Yadav, N., Lark, E., and Browse, J. (1994). Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell 6, 147-158.
23. Pidkowich, M. S., Nguyen, H. T., Heilmann, I., Ischebeck, T., and Shanklin, J. (2007). Modulating seed beta-ketoacyl-acyl carrier protein synthase II level converts the composition of a temperate seed oil to that of a palm-like tropical oil. Proc. Natl. Acad. Sci. U.S.A. 104, 4742-4747.
24. Qu, J., Ye, J., and Fang, R. (2007). Artificial microRNA-mediated virus resistance in plants. J. Virol. 81, 6690-6699.
25. Saheli, S., Jyoti Jha, K., Mrinal, K. M., Asitava, B., Ujjal, K. M., and Soumitra, K. S. (2007). Metabolic engineering of fatty acid biosynthesis in Indian mustard(Brassica juncea) improves nutritional quality of seed oil. Plant Biotechnol. Rep. 1, 185-197.
26. Schwab, R., Ossowski, S., Riester, M., Warthmann, N., and Weigel, D. (2006). Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18, 1121-1133.
27. Sellwood, C., Slabas, A. R., and Rawsthorne, S. (2000). Effects of manipulating expression of acetyl-CoA carboxylase I in Brassica napus L. embryos. Biochem. Soc. Trans. 28, 598-600.
28. Somerville, C. R., and Browse, J. A. (1991). Plant lipids: metabolism, mutants, and membranes. Science 252, 80-87.
29. Stoutjesdijk, P. A., Surinder, S. P., Liu, Q., Hurlstone, C. J., Waterhouse, P. A., and Green, A. G. (2002). hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiol. 129, 1723-1731.
30. Surinder, S., Green, A., Stoutjesdijk, P., and Liu, Q. (2000). Inverted-repeat DNA: a new gene-silencing tool for seed lipid modification. Biochem. Soc. Trans. 28, 925-927.
31. Tam, N., and John, S. (2009). Altering Arabidopsis oilseed composition by a combined antisense-hairpin RNAi gene suppression approach. J. Am. Oil Chem. Soc. 86, 41-49.
32. Tang, G., Galili, G., and Zhuang, X. (2007). RNAi, microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering. Metabolomics 3, 357-369.
33. Toppino, L., Kooiker, M., Lindner, M., Dreni, L., Rotino, G. L., and Kater, M. M. (2011). Reversible male sterility in eggplant (Solanum melongena L.) by artificial microRNA mediated silencing of general transcription factor genes. Plant Biotechnol. J. 9, 684-692.
34. Warthmann, N., Chen, H., Ossowski, S., Weigel, D., and Herve, P. (2008). Highly specific gene silencing by artificial miRNAs in rice. PLoS ONE 3, e1829. doi:10.1371/journal.pone.0001829
35. Zar, J. H. (2010). Biostatistical Analysis, 5th Edn. Upper Saddle River, NJ: Prentice-Hall.
36. Zhang, X., Li, H., Zhang, J., Zhang, C., Gong, P., Ziaf, K., Xiao, F., and Ye, Z. (2010). Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner. Transgenic Res. 3, 1-13.
37. Zhou, X., Singh, S., Liu, Q., and Green, A. (2006). Combined transgenic expression of δ12-desaturase and δ12-epoxygenase in high linoleic acid seeds leads to increased accumulation of vernolic acid. Funct. Plant Biol. 33, 585-592.