Increased levels of erucic acid in Brassica carinata by co-suppression and antisense repression of the endogenous FAD2 gene

Metabolic Engineering

Volumn 7, Issue 3, 2005, Pages 215-220

  Jadhav, Ashok ^a   Katavic, Vesna ^a   Marillia, Elizabeth France ^a   Giblin, E Michael ^a   Barton, Dennis L ^a   Kumar, Arvind ^a   Sonntag, Cory ^a   Babic, Vivijan ^a   Keller, Wilfred A ^a   Taylor, David C ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Antisense repression; Brassica carinata; Co suppression; FAD2; High erucic acid; Transformation

Indexed keywords

DERIVATIVES; GENES; OILS AND FATS; SEED;

HIGH ERUCIC ACIDS (HEA); SEED OILS; TRANSGENIC LINES; VERY LONG-CHAIN FATTY ACIDS (VLCFA);

FATTY ACIDS;

1 ACYL 2 OLEOYLGLYCERO 3 PHOSPHOCHOLINE DESATURASE; ERUCIC ACID; OLEIC ACID; POLYUNSATURATED FATTY ACID; UNCLASSIFIED DRUG; VEGETABLE PROTEIN; VERY LONG CHAIN FATTY ACID;

ARTICLE; BIOSYNTHESIS; BRASSICA; CONTROLLED STUDY; GENE EXPRESSION; GENE REPRESSION; GERMPLASM; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT BIOTECHNOLOGY; PRIORITY JOURNAL; STATISTICAL SIGNIFICANCE; TRANSGENIC PLANT;

BRASSICA; ERUCIC ACIDS; FATTY ACID DESATURASES; FATTY ACIDS; GENE SILENCING; GENETIC ENHANCEMENT; PROTEIN ENGINEERING; RNA, ANTISENSE; SUPPRESSION, GENETIC;

FATTY ACIDS; GENES; OIL; SEEDS;

BRASSICA CARINATA; BRASSICACEAE;

EID: 21044433278     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymben.2005.02.003     Document Type: Article

References (20)

1. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Stuhl Current Protocols in Molecular Biology vols. 1-3 1995 Wiley New York
2. V. Babic, R.S. Dalta, G.J. Scoles, and W.A. Keller Development of an efficient Agrobacterium-mediated transformation system for Brassica carinata Plant Cell Rep. 17 1998 183 188
3. R.S.S. Datla, J.K. Hammerlindl, B. Panchuk, L.E. Pelcher, and W.A. Keller Modified binary plant transformation vectors with the wild-type gene encoding NPTII Gene 211 1992 383 384
4. DeBonte, R., Hitz, W.D., 2000. US patent No. 6,063,947.
5. V. Katavic, D.W. Reed, D.C. Taylor, E.M. Giblin, D.L. Barton, J.-T. Zou, S.L. MacKenzie, P.S. Covello, and L. Kunst Alteration of seed fatty acid composition by an ethyl methanesulfonate-induced mutation in Arabidopsis thaliana affecting diacylglycerol acyltransferase activity Plant Physiol. 108 1995 399 409
6. V. Katavic, W. Friesen, L.D. Barton, K.K. Gossen, E.M. Giblin, T. Luciw, J. An, J.-T. Zou, S.L. MacKenzie, W.A. Keller, and C.D. Taylor Improving erucic acid content in rapeseed through biotechnology: what can the Arabidopsis FAE1 and Yeast SLC1-1 genes contribute? Crop Sci. 41 2001 739 747
7. E.P. Kennedy Biosynthesis of complex lipids Fed. Proc. Am. Soc. Exp. Biol. 20 1961 934 940
8. A.J. Kinney, and S. Knowlton Designer oils: the high oleic acid soybean S. Roller S. Harlander Genetic Modification in the Food Industry: A Strategy for Food Quality Improvement 1998 Chapman & Hall London 192 213
9. A.J. Kinney, E.B. Cahoon, and W.D. Hitz Manipulating desaturase activities in transgenic crop plants Biochem. Soc. Trans. 30 2002 1099 1103
10. L-DNA gene 5 controls the tissue-specific expression of chimaeric genes by a novel type of Agrobacterium binary vector Mol. Gen. Genet. 204 1986 383 396
11. C. Leonard Sources and commercial applications of high erucic vegetable oils Lipid Tech. 4 1994 79 83
12. J. Okuley, J. Lightner, K. Feldmann, N. Yadav, E. Lark, and J. Browse Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis Plant Cell 6 1994 147 158
13. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular cloning: A laboratory manual second ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor
14. N.O.V. Sonntag Erucic, behenic: feedstocks of the 21st century INFORM 5 1991 449 463
15. N.O.V. Sonntag Industrial utilization of long-chain fatty acids and their derivatives D.S. Kimber D.I. McGregor Brassica Oilseeds 1995 CAB International Oxon 339 352
16. E.M. Southern Detection of specific sequences among DNA fragments separated by gel electrophoresis J. Mol. Biol. 98 1975 503 517
17. P.A. Stoutjesdijk, C. Hurlestone, S.P. Singh, and A.G. Green High-oleic acid Australian Brassica napus and B. juncea varieties produced by co-suppression of endogenous Δ12-desaturases Biochem. Soc. Trans. 28 2000 938 940
18. D.C. Taylor, V. Katavic, J.-T. Zou, S.L. MacKenzie, W.A. Keller, J. An, W. Friesen, D.L. Barton, K.K. Gossen, E.M. Giblin, Y. Ge, M. Dauk, T. Luciw, and D. Males Field-testing of transgenic rapeseed cv. Hero transformed with a yeast sn-2 acyltransferase results in increased oil content, erucic acid content and seed yield Mol. Breeding 8 2002 317 322
19. Warwick, S.I., Black, L.D., 1993. Guide to the Wild Germplasm of Brassica and Allied Crops, Part III: Interspecific and Intergeneric Hybridization in the Tribe Brassiceae (Criciferae). Technical Bulletin 1993-16E, Center for Land and Biologiocal Resources Research, Agriculture and Agri-Food Canada.
20. J.-T. Zou, V. Katavic, E.M. Giblin, D.L. Barton, S.L. MacKenzie, W.A. Keller, X. Hu, and D.C. Taylor Modification of seed oil content and acyl composition in Brassicaceae by expression of a yeast sn-2 acyltransferase gene Plant Cell 9 1997 909 923