A novel extraction of trichosanthin from Trichosanthes kirilowii roots using three-phase partitioning and its in vitro anticancer activity

Pharmaceutical Biology

Volumn 52, Issue 6, 2014, Pages 677-680

  Mondal, Arijit ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

HepG2; MTT assay; SDS PAGE; WRL 68

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AMMONIUM SULFATE; BUTANOL; FLUOROURACIL; TRICHOSANTHIN; ANTINEOPLASTIC AGENT; PLANT EXTRACT;

ANTINEOPLASTIC ACTIVITY; ARTICLE; BIOASSAY; CANCER CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DRUG CYTOTOXICITY; HEPG2 CELL LINE; IC 50; IN VITRO STUDY; LIVER CANCER; PLANT ROOT; PROCESS OPTIMIZATION; SEPARATION TECHNIQUE; THREE PHASE PARTITIONING; TRICHOSANTHES; TRICHOSANTHES KIRILOWII; CELL SURVIVAL; DOSE RESPONSE; DRUG EFFECTS; HELA CELL LINE; HUMAN; ISOLATION AND PURIFICATION; PHYSIOLOGY;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; HELA CELLS; HEP G2 CELLS; HUMANS; PLANT EXTRACTS; PLANT ROOTS; TRICHOSANTHES; TRICHOSANTHIN;

EID: 84900793700     PISSN: 13880209     EISSN: 17445116     Source Type: Journal    
DOI: 10.3109/13880209.2013.864684     Document Type: Article

References (15)

1. El-Serag HB. (2004). Hepatocellular carcinoma: Recent trends in the United States. Gastroenterology 127:S27-34. (Pubitemid 39423367)
2. Harde SM, Singhal RS. (2012). Extraction of forskolin from Coleus forskohlii roots using three phase partitioning. Sep Purif Technol 96: 20-5.
3. Kiss E, Szamos J, Tamas B, Borbas R. (1998). Interfacial behavior of proteins in three phase partitioning using salt containing water/ tert-butanol systems. Colloids Surf 142:295-302. (Pubitemid 28529152)
4. Laemmli UK. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-5.
5. Li J, Xia X, Nie H, et al. (2007). PKC inhibition is involved in trichosanthin-induced apoptosis in human chronic myeloid leukemia cell line K562. Biochim Biophys Acta 1770:63-70. (Pubitemid 44920351)
6. Li YM, Chen JL, Yang AW, Luo R. (2007). Purification of EGF-TCS recombinant fusion protein and its targeting action on human tumor cells in vitro. Acta Acad Med Mil Tert 29:1316-19.
7. Mauceri HJ, Hanna NN, Beckett MA, et al. (1998). Combined effects of angiostatin and ionizing radiation in antitumour therapy. Nature 394: 287-91. (Pubitemid 28335042)
8. Narayan AV, Madhusudhan MC, Raghavarao KSMS. (2008). Extraction and purification of Ipomoea peroxidase employing three-phase partitioning. Appl Biochem Biotechnol 151:263-72.
9. Parkin DM, Bray F, Ferlay J, Pisani P. (2005). Global cancer statistics, 2002, CA. Cancer J Clin 55:74-105.
10. Paraskevi A, Ronald A. (2006). Hepatocellular carcinoma pathogenesis: From genes to environment. Nat Rev Cancer 6:1-14.
11. Schutte K Bornschein J Malfertheiner P. 2009 Hepatocellular carcinoma-epidemiological trends and risk factors Dig Dis 27 80-92.
12. Sharma A, Gupta MN. (2001). Three phase partitioning as a large-scale separation method for puriEcation of a wheat germ bifunctional protease/amylase inhibitor. Process Biochem 37:193-6. (Pubitemid 32880530)
13. Wang YY, Ouyang DY, Huang H, et al. (2005). Enhanced apoptotic action of trichosanthin in HIV-1 infected cell. Biochem Biophys Res Commun 331:1075-80. (Pubitemid 40692469)
14. Yates MS, Kensler TW. (2007). Keep 1 eye on the target: Chemoprevention of liver cancer. Acta Pharmacol Sin 28:1331-42. (Pubitemid 47366685)
15. Zhang C, Gong Y, Ma H, et al. (2001). Reactive oxygen species involved in trichosanthin-induced apoptosis of human choriocarcinoma cells. Biochem J 355:653-61. (Pubitemid 32434082)