Purification of protein complexes of defined subunit stoichiometry using a set of orthogonal, tag-cleaving proteases

Journal of Chromatography A

Volumn 1337, Issue , 2014, Pages 106-115

  Frey, Steffen ^a   Görlich, Dirk ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Affinity tag; Controlled subunit stoichiometry; On column cleavage; Protein complex; Tag removing protease

Indexed keywords

AFFINITY TAG; CLEAVAGE SPECIFICITY; COMPLEX ASSEMBLY; ON-COLUMN CLEAVAGE; PROTEIN COMPLEXES; PROTEIN PREPARATIONS; STRUCTURAL STUDIES; TAG-REMOVING PROTEASE;

PURIFICATION; STOICHIOMETRY;

PROTEINS;

PROTEIN BDNEDP1; PROTEIN BDSENP1; PROTEIN SCATG4; PROTEIN SCULP1; PROTEIN SSNEDP1; PROTEIN TEV; PROTEIN XLUSP2; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; IN VITRO STUDY; MOLECULAR RECOGNITION; NONHUMAN; PHASE SEPARATION; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; STOICHIOMETRY;

TOBACCO ETCH VIRUS;

AFFINITY TAG; CONTROLLED SUBUNIT STOICHIOMETRY; ON-COLUMN CLEAVAGE; PROTEIN COMPLEX; TAG-REMOVING PROTEASE;

CHROMATOGRAPHY, AFFINITY; HISTIDINE; MULTIPROTEIN COMPLEXES; PEPTIDE HYDROLASES; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SUMO-1 PROTEIN;

EID: 84900580299     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2014.02.030     Document Type: Article

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