Optimization of high grade glioma cell culture from surgical specimens for use in clinically relevant animal models and 3D immunochemistry

Journal of Visualized Experiments

Volumn , Issue 83, 2014, Pages

  Hasselbach, Laura A ^a   Irtenkauf, Susan M ^a   Lemke, Nancy W ^a   Nelson, Kevin K ^a   Berezovsky, Artem D ^a   Carlton, Enoch T ^a   Transou, Andrea D ^a   Mikkelsen, Tom ^a   deCarvalho, Ana C ^a  

^a HENRY FORD HOSPITAL   (United States)

Author keywords

Animal models; Glioblastoma; Issue 83; Long term self renewal; Medicine; Neoplasms; Nervous system diseases; Neurosphere; Primary cell culture; Surgical specimens

Indexed keywords

PARAFFIN;

ANIMAL; ARTICLE; BRAIN TUMOR; CANCER GRADING; CANCER STEM CELL; CULTURE MEDIUM; CULTURE TECHNIQUE; GLIOBLASTOMA; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY; MOUSE; MULTICELLULAR SPHEROID; NEURAL STEM CELL; PATHOLOGY;

ANIMALS; BRAIN NEOPLASMS; CELL CULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; GLIOBLASTOMA; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; NEOPLASM GRADING; NEOPLASTIC STEM CELLS; NEURAL STEM CELLS; PARAFFIN EMBEDDING; SPHEROIDS, CELLULAR;

EID: 84900450020     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51088     Document Type: Article

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